Isolation and characterization of glial filaments from human brain

J. E. Goldman, H. H. Schaumburg, W. T. Norton

Research output: Contribution to journalArticle

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Abstract

Intermediate (8-9 nm) filaments of human central nervous system astrocytes were isolated from the gliosed white matter of cases of adrenoleukodystrophy (ALD). This hereditary lipidosis is characterized pathologically by demyelination, loss of axons, and replacement of the white matter of the caudal cerebrum by a glial scar. Glial filaments were composed largely of a single protein component with a mol wt of about 49,000 daltons. Smaller components (44,000-39,000 daltons) were detected in some samples, and appear to represent degradation products of the filament protein. Human neurofilaments were isolated from the normal frontal white matter of ALD cases by the standard myelin-free axon technique. Isolated glial and neurofilament proteins comigrated during acrylamide gel electrophoresis in SDS. Polypeptides, resulting from cyanogen bromide cleavage of the two filament proteins were the same. Both proteins reacted with rabbit antisera raised against isolated bovine neurofilament protein and human glial fibrillary acidic protein.

Original languageEnglish (US)
Pages (from-to)426-440
Number of pages15
JournalJournal of Cell Biology
Volume78
Issue number2
StatePublished - 1978

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Neuroglia
Adrenoleukodystrophy
Neurofilament Proteins
Brain
Axons
Proteins
Lipidoses
Cyanogen Bromide
Intermediate Filaments
Acrylamide
Glial Fibrillary Acidic Protein
Cerebrum
Demyelinating Diseases
Myelin Sheath
Astrocytes
Cicatrix
Electrophoresis
Immune Sera
Central Nervous System
Gels

ASJC Scopus subject areas

  • Cell Biology

Cite this

Goldman, J. E., Schaumburg, H. H., & Norton, W. T. (1978). Isolation and characterization of glial filaments from human brain. Journal of Cell Biology, 78(2), 426-440.

Isolation and characterization of glial filaments from human brain. / Goldman, J. E.; Schaumburg, H. H.; Norton, W. T.

In: Journal of Cell Biology, Vol. 78, No. 2, 1978, p. 426-440.

Research output: Contribution to journalArticle

Goldman, JE, Schaumburg, HH & Norton, WT 1978, 'Isolation and characterization of glial filaments from human brain', Journal of Cell Biology, vol. 78, no. 2, pp. 426-440.
Goldman JE, Schaumburg HH, Norton WT. Isolation and characterization of glial filaments from human brain. Journal of Cell Biology. 1978;78(2):426-440.
Goldman, J. E. ; Schaumburg, H. H. ; Norton, W. T. / Isolation and characterization of glial filaments from human brain. In: Journal of Cell Biology. 1978 ; Vol. 78, No. 2. pp. 426-440.
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