Ionic, structural, and temperature effects on DNA nanoparticles formed by natural and synthetic polyamines

Veena Vijayanathan; Jasleen Lyall; Thresia Thomas; Akira Shirahata; T. J. Thomas

doi:10.1021/bm0493234

Ionic, structural, and temperature effects on DNA nanoparticles formed by natural and synthetic polyamines

Veena Vijayanathan, Jasleen Lyall, Thresia Thomas, Akira Shirahata, T. J. Thomas

Pediatrics

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

We synthesized analogues of spermine and studied the effects of chemical structure, ionic strength, and temperature on λ-DNA nanoparticle formation. Effective concentration of polyamines for DNA condensation (EC₅₀) was lowest for hexamines (0.2 μM) and highest for spermine (tetramine, 4.2 μM). The EC₅₀ value increased with [Na⁺]. Dynamic light scattering showed nanoparticles with hydrodynamic radii (R_h) of 40-50 nm. Effect of temperature on R_h was measured between 20 and 70 °C. For spermine, R_h remained relatively stable until 50 °C and increased significantly at >60 °C. In contrast, the hexa- and penta-valent analogues exhibited a gradual increase in R_h between 20 and 70 °C. The nanoparticles were mainly toroidal, as revealed by electron microscopy (EM). EM studies showed changes in morphology and size of condensed structures with an increase in temperature. A possible mechanism for the differential effects of temperature on DNA nanoparticles might involve different modes of DNA-polyamine interactions.

Original language	English (US)
Pages (from-to)	1097-1103
Number of pages	7
Journal	Biomacromolecules
Volume	6
Issue number	2
DOIs	https://doi.org/10.1021/bm0493234
State	Published - Mar 2005

ASJC Scopus subject areas

Bioengineering
Biomaterials
Polymers and Plastics
Materials Chemistry

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title = "Ionic, structural, and temperature effects on DNA nanoparticles formed by natural and synthetic polyamines",

abstract = "We synthesized analogues of spermine and studied the effects of chemical structure, ionic strength, and temperature on λ-DNA nanoparticle formation. Effective concentration of polyamines for DNA condensation (EC50) was lowest for hexamines (0.2 μM) and highest for spermine (tetramine, 4.2 μM). The EC50 value increased with [Na+]. Dynamic light scattering showed nanoparticles with hydrodynamic radii (Rh) of 40-50 nm. Effect of temperature on Rh was measured between 20 and 70 °C. For spermine, Rh remained relatively stable until 50 °C and increased significantly at >60 °C. In contrast, the hexa- and penta-valent analogues exhibited a gradual increase in Rh between 20 and 70 °C. The nanoparticles were mainly toroidal, as revealed by electron microscopy (EM). EM studies showed changes in morphology and size of condensed structures with an increase in temperature. A possible mechanism for the differential effects of temperature on DNA nanoparticles might involve different modes of DNA-polyamine interactions.",

author = "Veena Vijayanathan and Jasleen Lyall and Thresia Thomas and Akira Shirahata and Thomas, {T. J.}",

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T1 - Ionic, structural, and temperature effects on DNA nanoparticles formed by natural and synthetic polyamines

AU - Vijayanathan, Veena

AU - Lyall, Jasleen

AU - Thomas, Thresia

AU - Shirahata, Akira

AU - Thomas, T. J.

PY - 2005/3

Y1 - 2005/3

N2 - We synthesized analogues of spermine and studied the effects of chemical structure, ionic strength, and temperature on λ-DNA nanoparticle formation. Effective concentration of polyamines for DNA condensation (EC50) was lowest for hexamines (0.2 μM) and highest for spermine (tetramine, 4.2 μM). The EC50 value increased with [Na+]. Dynamic light scattering showed nanoparticles with hydrodynamic radii (Rh) of 40-50 nm. Effect of temperature on Rh was measured between 20 and 70 °C. For spermine, Rh remained relatively stable until 50 °C and increased significantly at >60 °C. In contrast, the hexa- and penta-valent analogues exhibited a gradual increase in Rh between 20 and 70 °C. The nanoparticles were mainly toroidal, as revealed by electron microscopy (EM). EM studies showed changes in morphology and size of condensed structures with an increase in temperature. A possible mechanism for the differential effects of temperature on DNA nanoparticles might involve different modes of DNA-polyamine interactions.

AB - We synthesized analogues of spermine and studied the effects of chemical structure, ionic strength, and temperature on λ-DNA nanoparticle formation. Effective concentration of polyamines for DNA condensation (EC50) was lowest for hexamines (0.2 μM) and highest for spermine (tetramine, 4.2 μM). The EC50 value increased with [Na+]. Dynamic light scattering showed nanoparticles with hydrodynamic radii (Rh) of 40-50 nm. Effect of temperature on Rh was measured between 20 and 70 °C. For spermine, Rh remained relatively stable until 50 °C and increased significantly at >60 °C. In contrast, the hexa- and penta-valent analogues exhibited a gradual increase in Rh between 20 and 70 °C. The nanoparticles were mainly toroidal, as revealed by electron microscopy (EM). EM studies showed changes in morphology and size of condensed structures with an increase in temperature. A possible mechanism for the differential effects of temperature on DNA nanoparticles might involve different modes of DNA-polyamine interactions.

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Ionic, structural, and temperature effects on DNA nanoparticles formed by natural and synthetic polyamines

Abstract

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