Ionic, structural, and temperature effects on DNA nanoparticles formed by natural and synthetic polyamines

Veena Vijayanathan, Jasleen Lyall, Thresia Thomas, Akira Shirahata, T. J. Thomas

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

We synthesized analogues of spermine and studied the effects of chemical structure, ionic strength, and temperature on λ-DNA nanoparticle formation. Effective concentration of polyamines for DNA condensation (EC50) was lowest for hexamines (0.2 μM) and highest for spermine (tetramine, 4.2 μM). The EC50 value increased with [Na+]. Dynamic light scattering showed nanoparticles with hydrodynamic radii (Rh) of 40-50 nm. Effect of temperature on Rh was measured between 20 and 70 °C. For spermine, Rh remained relatively stable until 50 °C and increased significantly at >60 °C. In contrast, the hexa- and penta-valent analogues exhibited a gradual increase in Rh between 20 and 70 °C. The nanoparticles were mainly toroidal, as revealed by electron microscopy (EM). EM studies showed changes in morphology and size of condensed structures with an increase in temperature. A possible mechanism for the differential effects of temperature on DNA nanoparticles might involve different modes of DNA-polyamine interactions.

Original languageEnglish (US)
Pages (from-to)1097-1103
Number of pages7
JournalBiomacromolecules
Volume6
Issue number2
DOIs
StatePublished - Mar 2005
Externally publishedYes

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Polyamines
Thermal effects
Nanoparticles
Spermine
DNA
tetramethylenedisulfotetramine
Temperature
Electron microscopy
Electron Microscopy
Methenamine
Rubiaceae
Dynamic light scattering
Hydrodynamics
Ionic strength
Osmolar Concentration
Condensation

ASJC Scopus subject areas

  • Organic Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Ionic, structural, and temperature effects on DNA nanoparticles formed by natural and synthetic polyamines. / Vijayanathan, Veena; Lyall, Jasleen; Thomas, Thresia; Shirahata, Akira; Thomas, T. J.

In: Biomacromolecules, Vol. 6, No. 2, 03.2005, p. 1097-1103.

Research output: Contribution to journalArticle

Vijayanathan, V, Lyall, J, Thomas, T, Shirahata, A & Thomas, TJ 2005, 'Ionic, structural, and temperature effects on DNA nanoparticles formed by natural and synthetic polyamines', Biomacromolecules, vol. 6, no. 2, pp. 1097-1103. https://doi.org/10.1021/bm0493234
Vijayanathan, Veena ; Lyall, Jasleen ; Thomas, Thresia ; Shirahata, Akira ; Thomas, T. J. / Ionic, structural, and temperature effects on DNA nanoparticles formed by natural and synthetic polyamines. In: Biomacromolecules. 2005 ; Vol. 6, No. 2. pp. 1097-1103.
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