Ionic coupling and mitotic synchrony of siblings in a Drosophila cell line

David C. Spray, Lucy Cherbas, Peter Cherbas, E. A. Morales, Grant M. Carrow

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Abstract

Following mitosis in many cell lines, siblings remain adjoined in dyads until further cell division. We report here a series of experiments designed to ascertain the nature of this apposition in the embryonic Kc cell line of Drosophila melanogaster. We have found that (1) cell division in siblings is highly synchronized when compared to that in nonsiblings; (2) siblings in dyads are dye coupled with respect to Lucifer Yellow, but intercellular diffusion of larger molecules (FITC-dextran at 6 and 24 kDa) is retarded; (3) siblings are electrically coupled by an ungated, low-resistance intercellular connection which is resistant to treatment with octanol and CO2, both known to close gap junction channels; and (4) members of a dyad are joined by a cytoplasmic bridge. Structures resembling septate junctions are also found between siblings and between cells in aggregates. The evidence accumulated here suggests that cytokinesis in Kc dyads is incomplete, resulting in an intercellular pathway that may provide for the passage of a molecular or electrical signal that regulates subsequent mitosis.

Original languageEnglish (US)
Pages (from-to)509-517
Number of pages9
JournalExperimental Cell Research
Volume184
Issue number2
DOIs
Publication statusPublished - Oct 1989

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ASJC Scopus subject areas

  • Cell Biology

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