Ionic coupling and mitotic synchrony of siblings in a Drosophila cell line

David C. Spray, Lucy Cherbas, Peter Cherbas, E. A. Morales, Grant M. Carrow

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Following mitosis in many cell lines, siblings remain adjoined in dyads until further cell division. We report here a series of experiments designed to ascertain the nature of this apposition in the embryonic Kc cell line of Drosophila melanogaster. We have found that (1) cell division in siblings is highly synchronized when compared to that in nonsiblings; (2) siblings in dyads are dye coupled with respect to Lucifer Yellow, but intercellular diffusion of larger molecules (FITC-dextran at 6 and 24 kDa) is retarded; (3) siblings are electrically coupled by an ungated, low-resistance intercellular connection which is resistant to treatment with octanol and CO2, both known to close gap junction channels; and (4) members of a dyad are joined by a cytoplasmic bridge. Structures resembling septate junctions are also found between siblings and between cells in aggregates. The evidence accumulated here suggests that cytokinesis in Kc dyads is incomplete, resulting in an intercellular pathway that may provide for the passage of a molecular or electrical signal that regulates subsequent mitosis.

Original languageEnglish (US)
Pages (from-to)509-517
Number of pages9
JournalExperimental Cell Research
Volume184
Issue number2
DOIs
StatePublished - 1989

Fingerprint

Mitosis
Cell Division
Drosophila
Octanols
Cell Line
Cytokinesis
Gap Junctions
Drosophila melanogaster
Coloring Agents
fluorescein isothiocyanate dextran
lucifer yellow

ASJC Scopus subject areas

  • Cell Biology

Cite this

Ionic coupling and mitotic synchrony of siblings in a Drosophila cell line. / Spray, David C.; Cherbas, Lucy; Cherbas, Peter; Morales, E. A.; Carrow, Grant M.

In: Experimental Cell Research, Vol. 184, No. 2, 1989, p. 509-517.

Research output: Contribution to journalArticle

Spray, David C. ; Cherbas, Lucy ; Cherbas, Peter ; Morales, E. A. ; Carrow, Grant M. / Ionic coupling and mitotic synchrony of siblings in a Drosophila cell line. In: Experimental Cell Research. 1989 ; Vol. 184, No. 2. pp. 509-517.
@article{f067180317bf40c3b2eb686cefc2de6d,
title = "Ionic coupling and mitotic synchrony of siblings in a Drosophila cell line",
abstract = "Following mitosis in many cell lines, siblings remain adjoined in dyads until further cell division. We report here a series of experiments designed to ascertain the nature of this apposition in the embryonic Kc cell line of Drosophila melanogaster. We have found that (1) cell division in siblings is highly synchronized when compared to that in nonsiblings; (2) siblings in dyads are dye coupled with respect to Lucifer Yellow, but intercellular diffusion of larger molecules (FITC-dextran at 6 and 24 kDa) is retarded; (3) siblings are electrically coupled by an ungated, low-resistance intercellular connection which is resistant to treatment with octanol and CO2, both known to close gap junction channels; and (4) members of a dyad are joined by a cytoplasmic bridge. Structures resembling septate junctions are also found between siblings and between cells in aggregates. The evidence accumulated here suggests that cytokinesis in Kc dyads is incomplete, resulting in an intercellular pathway that may provide for the passage of a molecular or electrical signal that regulates subsequent mitosis.",
author = "Spray, {David C.} and Lucy Cherbas and Peter Cherbas and Morales, {E. A.} and Carrow, {Grant M.}",
year = "1989",
doi = "10.1016/0014-4827(89)90348-0",
language = "English (US)",
volume = "184",
pages = "509--517",
journal = "Experimental Cell Research",
issn = "0014-4827",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Ionic coupling and mitotic synchrony of siblings in a Drosophila cell line

AU - Spray, David C.

AU - Cherbas, Lucy

AU - Cherbas, Peter

AU - Morales, E. A.

AU - Carrow, Grant M.

PY - 1989

Y1 - 1989

N2 - Following mitosis in many cell lines, siblings remain adjoined in dyads until further cell division. We report here a series of experiments designed to ascertain the nature of this apposition in the embryonic Kc cell line of Drosophila melanogaster. We have found that (1) cell division in siblings is highly synchronized when compared to that in nonsiblings; (2) siblings in dyads are dye coupled with respect to Lucifer Yellow, but intercellular diffusion of larger molecules (FITC-dextran at 6 and 24 kDa) is retarded; (3) siblings are electrically coupled by an ungated, low-resistance intercellular connection which is resistant to treatment with octanol and CO2, both known to close gap junction channels; and (4) members of a dyad are joined by a cytoplasmic bridge. Structures resembling septate junctions are also found between siblings and between cells in aggregates. The evidence accumulated here suggests that cytokinesis in Kc dyads is incomplete, resulting in an intercellular pathway that may provide for the passage of a molecular or electrical signal that regulates subsequent mitosis.

AB - Following mitosis in many cell lines, siblings remain adjoined in dyads until further cell division. We report here a series of experiments designed to ascertain the nature of this apposition in the embryonic Kc cell line of Drosophila melanogaster. We have found that (1) cell division in siblings is highly synchronized when compared to that in nonsiblings; (2) siblings in dyads are dye coupled with respect to Lucifer Yellow, but intercellular diffusion of larger molecules (FITC-dextran at 6 and 24 kDa) is retarded; (3) siblings are electrically coupled by an ungated, low-resistance intercellular connection which is resistant to treatment with octanol and CO2, both known to close gap junction channels; and (4) members of a dyad are joined by a cytoplasmic bridge. Structures resembling septate junctions are also found between siblings and between cells in aggregates. The evidence accumulated here suggests that cytokinesis in Kc dyads is incomplete, resulting in an intercellular pathway that may provide for the passage of a molecular or electrical signal that regulates subsequent mitosis.

UR - http://www.scopus.com/inward/record.url?scp=0024457812&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024457812&partnerID=8YFLogxK

U2 - 10.1016/0014-4827(89)90348-0

DO - 10.1016/0014-4827(89)90348-0

M3 - Article

VL - 184

SP - 509

EP - 517

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

IS - 2

ER -