Involvement of striatal lipid peroxidation and inhibition of calcium influx into brain slices in neurobehavioral alterations in a rat model of short-term oral exposure to manganese

Daiana Silva Ávila, Priscila Gubert, Roselei Fachinetto, Caroline Wagner, Michael Aschner, João Batista Teixeira Rocha, Félix Alexandre Antunes Soares

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Manganese is an essential element for biological systems, nevertheless occupational exposure to high levels of Mn can lead to neurodegenerative disorder, characterized by excessive Mn accumulation, especially in astrocytes of basal ganglia and symptoms closely resembling idiopathic Parkinson's disease (PD). The purpose of this study was to evaluate behavioral and biochemical alterations in adult rats exposed for 30 days to 10 and 25 mg/mL of MnCl2 in their drinking water. MnCl2 intoxicated rats showed impaired locomotor activity in comparison to control animals. Furthermore, lipid peroxidation were increased, δ-aminolevulinate dehydratase (δ-ALA-D, an enzyme sensitive to pro-oxidant situations) activity was inhibited and 45Ca2+ influx into striatal slices was decreased in rats exposed to 25 mg/mL of Mn, indicating that this brain region was markedly affected by short-term Mn exposure. In contrast, Mn exposure was not associated with characteristic extrapyramidal effects and did not modify protein oxidation, suggesting that the striatal damage represents early stages of Mn-induced damage. In addition, treatment with Mn was associated with reduced body weight gain, but there were no discernible alterations in liver and kidney function. In conclusion, Mn caused increased oxidative stress and decreased 45Ca2+ influx into the striatum, which are likely linked to impaired locomotor activity, but not with the occurrence of orofacial dyskinesia.

Original languageEnglish (US)
Pages (from-to)1062-1068
Number of pages7
JournalNeuroToxicology
Volume29
Issue number6
DOIs
StatePublished - 2008
Externally publishedYes

Fingerprint

Corpus Striatum
Manganese
Lipid Peroxidation
Rats
Brain
Locomotion
Calcium
4 alpha-glucanotransferase
Lipids
Hydro-Lyases
Oxidative stress
Dyskinesias
Biological systems
Occupational Exposure
Basal Ganglia
Astrocytes
Drinking Water
Neurodegenerative Diseases
Liver
Weight Gain

Keywords

  • Behavior alterations
  • Calcium influx
  • Manganese
  • Oral short-term exposure
  • Oxidative stress
  • Striatum

ASJC Scopus subject areas

  • Neuroscience(all)
  • Toxicology

Cite this

Involvement of striatal lipid peroxidation and inhibition of calcium influx into brain slices in neurobehavioral alterations in a rat model of short-term oral exposure to manganese. / Ávila, Daiana Silva; Gubert, Priscila; Fachinetto, Roselei; Wagner, Caroline; Aschner, Michael; Rocha, João Batista Teixeira; Soares, Félix Alexandre Antunes.

In: NeuroToxicology, Vol. 29, No. 6, 2008, p. 1062-1068.

Research output: Contribution to journalArticle

Ávila, Daiana Silva ; Gubert, Priscila ; Fachinetto, Roselei ; Wagner, Caroline ; Aschner, Michael ; Rocha, João Batista Teixeira ; Soares, Félix Alexandre Antunes. / Involvement of striatal lipid peroxidation and inhibition of calcium influx into brain slices in neurobehavioral alterations in a rat model of short-term oral exposure to manganese. In: NeuroToxicology. 2008 ; Vol. 29, No. 6. pp. 1062-1068.
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