Involvement of Histamine in Endothelium-Dependent Relaxation of Mesenteric Lymphatic Vessels

Irina Tsoy Nizamutdinova, Daisuke Maejima, Takashi Nagai, Eric Bridenbaugh, Sangeetha Thangaswamy, Victor Chatterjee, Cynthia J. Meininger, Anatoliy A. Gashev

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Objectives: The knowledge of the basic principles of lymphatic function, still remains, to a large degree, rudimentary and will require significant research efforts. Recent studies of the physiology of the MLVs suggested the presence of an EDRF other than NO. In this study, we tested the hypothesis that lymphatic endothelium-derived histamine relaxes MLVs. Methods: We measured and analyzed parameters of lymphatic contractility in isolated and pressurized rat MLVs under control conditions and after pharmacological blockade of NO by l-NAME (100 μM) or/and histamine production by α-MHD (10 μM). Effectiveness of α-MHD was confirmed immunohistochemically. We also used immunohistochemical labeling and Western blot analysis of the histamine-producing enzyme, HDC. In addition, we blocked HDC protein expression in MLVs by transient transfection with vivo-morpholino oligos. Results: We found that only combined pharmacological blockade of NO and histamine production completely eliminates flow-dependent relaxation of lymphatic vessels, thus confirming a role for histamine as an EDRF in MLVs. We also confirmed the presence of HDC and histamine inside lymphatic endothelial cells. Conclusions: This study supports a role for histamine as an EDRF in MLVs.

Original languageEnglish (US)
Pages (from-to)640-648
Number of pages9
JournalMicrocirculation
Volume21
Issue number7
DOIs
StatePublished - Oct 1 2014
Externally publishedYes

Fingerprint

Lymphatic Vessels
Histamine
Endothelium
Lymphatic Endothelium
Pharmacology
Morpholinos
Transfection
Endothelial Cells
Western Blotting
Enzymes
Research

Keywords

  • EDRF
  • Histamine
  • Lymphatic endothelium
  • Mesenteric lymphatic vessels

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Nizamutdinova, I. T., Maejima, D., Nagai, T., Bridenbaugh, E., Thangaswamy, S., Chatterjee, V., ... Gashev, A. A. (2014). Involvement of Histamine in Endothelium-Dependent Relaxation of Mesenteric Lymphatic Vessels. Microcirculation, 21(7), 640-648. https://doi.org/10.1111/micc.12143

Involvement of Histamine in Endothelium-Dependent Relaxation of Mesenteric Lymphatic Vessels. / Nizamutdinova, Irina Tsoy; Maejima, Daisuke; Nagai, Takashi; Bridenbaugh, Eric; Thangaswamy, Sangeetha; Chatterjee, Victor; Meininger, Cynthia J.; Gashev, Anatoliy A.

In: Microcirculation, Vol. 21, No. 7, 01.10.2014, p. 640-648.

Research output: Contribution to journalArticle

Nizamutdinova, IT, Maejima, D, Nagai, T, Bridenbaugh, E, Thangaswamy, S, Chatterjee, V, Meininger, CJ & Gashev, AA 2014, 'Involvement of Histamine in Endothelium-Dependent Relaxation of Mesenteric Lymphatic Vessels', Microcirculation, vol. 21, no. 7, pp. 640-648. https://doi.org/10.1111/micc.12143
Nizamutdinova IT, Maejima D, Nagai T, Bridenbaugh E, Thangaswamy S, Chatterjee V et al. Involvement of Histamine in Endothelium-Dependent Relaxation of Mesenteric Lymphatic Vessels. Microcirculation. 2014 Oct 1;21(7):640-648. https://doi.org/10.1111/micc.12143
Nizamutdinova, Irina Tsoy ; Maejima, Daisuke ; Nagai, Takashi ; Bridenbaugh, Eric ; Thangaswamy, Sangeetha ; Chatterjee, Victor ; Meininger, Cynthia J. ; Gashev, Anatoliy A. / Involvement of Histamine in Endothelium-Dependent Relaxation of Mesenteric Lymphatic Vessels. In: Microcirculation. 2014 ; Vol. 21, No. 7. pp. 640-648.
@article{b7241cfb52b642a7a13dded0a1fd697d,
title = "Involvement of Histamine in Endothelium-Dependent Relaxation of Mesenteric Lymphatic Vessels",
abstract = "Objectives: The knowledge of the basic principles of lymphatic function, still remains, to a large degree, rudimentary and will require significant research efforts. Recent studies of the physiology of the MLVs suggested the presence of an EDRF other than NO. In this study, we tested the hypothesis that lymphatic endothelium-derived histamine relaxes MLVs. Methods: We measured and analyzed parameters of lymphatic contractility in isolated and pressurized rat MLVs under control conditions and after pharmacological blockade of NO by l-NAME (100 μM) or/and histamine production by α-MHD (10 μM). Effectiveness of α-MHD was confirmed immunohistochemically. We also used immunohistochemical labeling and Western blot analysis of the histamine-producing enzyme, HDC. In addition, we blocked HDC protein expression in MLVs by transient transfection with vivo-morpholino oligos. Results: We found that only combined pharmacological blockade of NO and histamine production completely eliminates flow-dependent relaxation of lymphatic vessels, thus confirming a role for histamine as an EDRF in MLVs. We also confirmed the presence of HDC and histamine inside lymphatic endothelial cells. Conclusions: This study supports a role for histamine as an EDRF in MLVs.",
keywords = "EDRF, Histamine, Lymphatic endothelium, Mesenteric lymphatic vessels",
author = "Nizamutdinova, {Irina Tsoy} and Daisuke Maejima and Takashi Nagai and Eric Bridenbaugh and Sangeetha Thangaswamy and Victor Chatterjee and Meininger, {Cynthia J.} and Gashev, {Anatoliy A.}",
year = "2014",
month = "10",
day = "1",
doi = "10.1111/micc.12143",
language = "English (US)",
volume = "21",
pages = "640--648",
journal = "Microcirculation",
issn = "1073-9688",
publisher = "Wiley-Blackwell",
number = "7",

}

TY - JOUR

T1 - Involvement of Histamine in Endothelium-Dependent Relaxation of Mesenteric Lymphatic Vessels

AU - Nizamutdinova, Irina Tsoy

AU - Maejima, Daisuke

AU - Nagai, Takashi

AU - Bridenbaugh, Eric

AU - Thangaswamy, Sangeetha

AU - Chatterjee, Victor

AU - Meininger, Cynthia J.

AU - Gashev, Anatoliy A.

PY - 2014/10/1

Y1 - 2014/10/1

N2 - Objectives: The knowledge of the basic principles of lymphatic function, still remains, to a large degree, rudimentary and will require significant research efforts. Recent studies of the physiology of the MLVs suggested the presence of an EDRF other than NO. In this study, we tested the hypothesis that lymphatic endothelium-derived histamine relaxes MLVs. Methods: We measured and analyzed parameters of lymphatic contractility in isolated and pressurized rat MLVs under control conditions and after pharmacological blockade of NO by l-NAME (100 μM) or/and histamine production by α-MHD (10 μM). Effectiveness of α-MHD was confirmed immunohistochemically. We also used immunohistochemical labeling and Western blot analysis of the histamine-producing enzyme, HDC. In addition, we blocked HDC protein expression in MLVs by transient transfection with vivo-morpholino oligos. Results: We found that only combined pharmacological blockade of NO and histamine production completely eliminates flow-dependent relaxation of lymphatic vessels, thus confirming a role for histamine as an EDRF in MLVs. We also confirmed the presence of HDC and histamine inside lymphatic endothelial cells. Conclusions: This study supports a role for histamine as an EDRF in MLVs.

AB - Objectives: The knowledge of the basic principles of lymphatic function, still remains, to a large degree, rudimentary and will require significant research efforts. Recent studies of the physiology of the MLVs suggested the presence of an EDRF other than NO. In this study, we tested the hypothesis that lymphatic endothelium-derived histamine relaxes MLVs. Methods: We measured and analyzed parameters of lymphatic contractility in isolated and pressurized rat MLVs under control conditions and after pharmacological blockade of NO by l-NAME (100 μM) or/and histamine production by α-MHD (10 μM). Effectiveness of α-MHD was confirmed immunohistochemically. We also used immunohistochemical labeling and Western blot analysis of the histamine-producing enzyme, HDC. In addition, we blocked HDC protein expression in MLVs by transient transfection with vivo-morpholino oligos. Results: We found that only combined pharmacological blockade of NO and histamine production completely eliminates flow-dependent relaxation of lymphatic vessels, thus confirming a role for histamine as an EDRF in MLVs. We also confirmed the presence of HDC and histamine inside lymphatic endothelial cells. Conclusions: This study supports a role for histamine as an EDRF in MLVs.

KW - EDRF

KW - Histamine

KW - Lymphatic endothelium

KW - Mesenteric lymphatic vessels

UR - http://www.scopus.com/inward/record.url?scp=84907203122&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84907203122&partnerID=8YFLogxK

U2 - 10.1111/micc.12143

DO - 10.1111/micc.12143

M3 - Article

C2 - 24750494

AN - SCOPUS:84907203122

VL - 21

SP - 640

EP - 648

JO - Microcirculation

JF - Microcirculation

SN - 1073-9688

IS - 7

ER -