Invasion of human breast cancer cells in vivo requires both paracrine and autocrine loops involving the colony-stimulating factor-1 receptor

Antonia Patsialou, Jeffrey Wyckoff, Yarong Wang, Sumanta Goswami, E. Richard Stanley, John S. Condeelis

Research output: Contribution to journalArticle

146 Citations (Scopus)

Abstract

Colony-stimulating factor-1 (CSF-1) and its receptor (CSF-1R) have been implicated in the pathogenesis and progression of various types of cancer, including breast cancer. This is based on high levels of circulating CSF-1 in patient sera with aggressive disease and increased CSF-1R staining in the tumor tissues. However, there have been no direct in vivo studies to determine whether a CSF-1 autocrine signaling loop functions in human breast cancer cells in vivo and whether it contributes to invasion. Recently, in mouse and rat models, it has been shown that invasion and metastasis are driven by an epidermal growth factor (EGF)/CSF-1 paracrine loop between tumor cells and host macrophages. In this macrophagedependent invasion, tumor cells secrete CSF-1 and sense EGF, whereas the macrophages secrete EGF and sense CSF-1. Here, we test the hypothesis that in human breast tumors, the expression of both the CSF-1 ligand and its receptor in tumor cells leads to a CSF-1/CSF-1R autocrine loop which contributes to the aggressive phenotype of human breast tumors. Using MDA-MB-231 cell-derived mammary tumors in severe combined immunodeficiency mice, we show here for the first time in vivo that invasion in a human mammary tumor model is dependent on both paracrine signaling with host macrophages as well as autocrine signaling involving the tumor cells themselves. In particular, we show that the autocrine contribution to invasion is specifically amplified in vivo through a tumor microenvironment-induced upregulation of CSF-1R expression via the transforming growth factor-β1.

Original languageEnglish (US)
Pages (from-to)9498-9506
Number of pages9
JournalCancer Research
Volume69
Issue number24
DOIs
StatePublished - Dec 15 2009

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Colony-Stimulating Factor Receptors
Macrophage Colony-Stimulating Factor
Breast Neoplasms
Macrophage Colony-Stimulating Factor Receptors
Autocrine Communication
Epidermal Growth Factor
Macrophages
Neoplasms
Paracrine Communication
Severe Combined Immunodeficiency
Tumor Microenvironment
Transforming Growth Factors
Up-Regulation
Staining and Labeling
Neoplasm Metastasis
Ligands
Phenotype

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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Invasion of human breast cancer cells in vivo requires both paracrine and autocrine loops involving the colony-stimulating factor-1 receptor. / Patsialou, Antonia; Wyckoff, Jeffrey; Wang, Yarong; Goswami, Sumanta; Stanley, E. Richard; Condeelis, John S.

In: Cancer Research, Vol. 69, No. 24, 15.12.2009, p. 9498-9506.

Research output: Contribution to journalArticle

Patsialou, Antonia ; Wyckoff, Jeffrey ; Wang, Yarong ; Goswami, Sumanta ; Stanley, E. Richard ; Condeelis, John S. / Invasion of human breast cancer cells in vivo requires both paracrine and autocrine loops involving the colony-stimulating factor-1 receptor. In: Cancer Research. 2009 ; Vol. 69, No. 24. pp. 9498-9506.
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