Interrupted replication of hepatitis B virus in liver tissue of HBsAg carriers with hepatocellular carcinoma

Giovanni Raimondo, Robert D. Burk, Harvey M. Lieberman, Joseph Muschel, Stephanos J. Hadziyannis, Hans Will, Michael C. Kew, Geoffrey M. Dusheiko, David A. Shafritz

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

To search for events underlying reduction of peripheral viremia and integration of hepatitis B virus (HBV) DNA into the liver cell genome in long-term virus carriers with hepatocellular carcinoma, paired samples of liver and tumor tissue were analyzed by molecular hybridization and immunological methods. Most tumor tissues contained integrated viral DNA; in none was extrachromosomal HBV DNA detected. Integrated HBV DNA was also found in peritumor liver tissue in the majority of patients. However, liver of patients either with or without peripheral viremia also contained free HBV DNA and replicative intermediates. In three nonviremic patients with replicative HBV DNA in liver, viral core antigen expression was markedly reduced or absent, whereas viral envelope protein (surface antigen) expression was normal. In one case, replicative intermediates in liver were sensitive to DNase I digestion, indicating that viral DNA was not encapsidated in normal viral core particles. These results suggest that decreased or defective core antigen production can lead to reduced viremia associated with blocked virus assembly/secretion and accumulation of unencapsidated HBV DNA replicative intermediates in the liver cell. Accumulation of such HBV DNA molecular forms in the liver may lead to an increased propensity for HBV DNA to integrate into the host genome, which has been found with high frequency in hepatic neoplasms from patients infected with hepatitis B virus.

Original languageEnglish (US)
Pages (from-to)103-112
Number of pages10
JournalVirology
Volume166
Issue number1
DOIs
StatePublished - 1988

Fingerprint

Hepatitis B Surface Antigens
Hepatitis B virus
Hepatocellular Carcinoma
Liver
DNA
Viremia
Viral DNA
Genome
Viral Envelope Proteins
Virus Assembly
Viral Antigens
Deoxyribonuclease I
Surface Antigens
Liver Neoplasms
Virion
Digestion
Neoplasms
Viruses
Antigens

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Raimondo, G., Burk, R. D., Lieberman, H. M., Muschel, J., Hadziyannis, S. J., Will, H., ... Shafritz, D. A. (1988). Interrupted replication of hepatitis B virus in liver tissue of HBsAg carriers with hepatocellular carcinoma. Virology, 166(1), 103-112. https://doi.org/10.1016/0042-6822(88)90151-1

Interrupted replication of hepatitis B virus in liver tissue of HBsAg carriers with hepatocellular carcinoma. / Raimondo, Giovanni; Burk, Robert D.; Lieberman, Harvey M.; Muschel, Joseph; Hadziyannis, Stephanos J.; Will, Hans; Kew, Michael C.; Dusheiko, Geoffrey M.; Shafritz, David A.

In: Virology, Vol. 166, No. 1, 1988, p. 103-112.

Research output: Contribution to journalArticle

Raimondo, G, Burk, RD, Lieberman, HM, Muschel, J, Hadziyannis, SJ, Will, H, Kew, MC, Dusheiko, GM & Shafritz, DA 1988, 'Interrupted replication of hepatitis B virus in liver tissue of HBsAg carriers with hepatocellular carcinoma', Virology, vol. 166, no. 1, pp. 103-112. https://doi.org/10.1016/0042-6822(88)90151-1
Raimondo, Giovanni ; Burk, Robert D. ; Lieberman, Harvey M. ; Muschel, Joseph ; Hadziyannis, Stephanos J. ; Will, Hans ; Kew, Michael C. ; Dusheiko, Geoffrey M. ; Shafritz, David A. / Interrupted replication of hepatitis B virus in liver tissue of HBsAg carriers with hepatocellular carcinoma. In: Virology. 1988 ; Vol. 166, No. 1. pp. 103-112.
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