Interphase and metaphase resolution of different distances within the human dystrophin gene

Jeanne Bentley Lawrence, Robert H. Singer, John A. McNeil

Research output: Contribution to journalArticle

222 Scopus citations

Abstract

Fluorescence in situ hybridization makes possible direct visualization of single sequences not only on chromosomes, but within decondensed interphase nuclei, providing a potentially powerful approach for high-resolution (1 Mb and below) gene mapping and the analysis of nuclear organization. Interphase mapping was able to extend the ability to resolve and order sequences up to two orders of magnitude beyond localization on banded or unbanded chromosomes. Sequences within the human dystrophin gene separated by <100 kb to 1 Mb were visually resolved at interphase by means of standard microscopy. In contrast, distances in the 1-Mb range could not be ordered on the metaphase chromosome length. Analysis ofsequences 100 kb to 1 Mb apart indicates a strong correlation between interphase distance and linear DNA distance, which could facilitate a variety of gene-mapping efforts. Results estimate chromatin condensation up to 1 Mb and indicate a comparable condensation for different cell types prepared by different techniques.

Original languageEnglish (US)
Pages (from-to)928-932
Number of pages5
JournalScience
Volume249
Issue number4971
DOIs
StatePublished - 1990

ASJC Scopus subject areas

  • General

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