Interphase and metaphase resolution of different distances within the human dystrophin gene

Jeanne Bentley Lawrence, Robert H. Singer, John A. McNeil

Research output: Contribution to journalArticle

221 Citations (Scopus)

Abstract

Fluorescence in situ hybridization makes possible direct visualization of single sequences not only on chromosomes, but within decondensed interphase nuclei, providing a potentially powerful approach for high-resolution (1 Mb and below) gene mapping and the analysis of nuclear organization. Interphase mapping was able to extend the ability to resolve and order sequences up to two orders of magnitude beyond localization on banded or unbanded chromosomes. Sequences within the human dystrophin gene separated by <100 kb to 1 Mb were visually resolved at interphase by means of standard microscopy. In contrast, distances in the 1-Mb range could not be ordered on the metaphase chromosome length. Analysis of sequences 100 kb to 1 Mb apart indicates a strong correlation between interphase distance and linear DNA distance, which could facilitate a variety of gene-mapping efforts. Results estimate chromatin condensation up to 1 Mb and indicate a comparable condensation for different cell types prepared by different techniques.

Original languageEnglish (US)
Pages (from-to)928-932
Number of pages5
JournalScience
Volume249
Issue number4971
StatePublished - Aug 24 1990
Externally publishedYes

Fingerprint

Dystrophin
Interphase
Metaphase
Chromosomes
Chromosome Mapping
Genes
Fluorescence In Situ Hybridization
Chromatin
Sequence Analysis
Microscopy
DNA

ASJC Scopus subject areas

  • General

Cite this

Interphase and metaphase resolution of different distances within the human dystrophin gene. / Lawrence, Jeanne Bentley; Singer, Robert H.; McNeil, John A.

In: Science, Vol. 249, No. 4971, 24.08.1990, p. 928-932.

Research output: Contribution to journalArticle

Lawrence, Jeanne Bentley ; Singer, Robert H. ; McNeil, John A. / Interphase and metaphase resolution of different distances within the human dystrophin gene. In: Science. 1990 ; Vol. 249, No. 4971. pp. 928-932.
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