Interleukin 2 production in vitro by peripheral lymphocytes in response to human papillomavirus-derived peptides: Correlation with cervical pathology

Taku Tsukui; Allan Hildesheim; Mark H. Schiffman; Joseph Lucci; David Contois; Patricia Lawler; Brenda B. Rush; Attila T. Lorincz; Alice Corrigan; Robert D. Burk; Weimin Qu; Margaret A. Marshall; Dean Mann; Mary Carrington; Mario Clerici; Gene M. Shearer; David P. Carbone; David R. Scott; Richard A. Houghten; Jay A. Berzofsky

Interleukin 2 production in vitro by peripheral lymphocytes in response to human papillomavirus-derived peptides: Correlation with cervical pathology

Taku Tsukui, Allan Hildesheim, Mark H. Schiffman, Joseph Lucci, David Contois, Patricia Lawler, Brenda B. Rush, Attila T. Lorincz, Alice Corrigan, Robert D. Burk, Weimin Qu, Margaret A. Marshall, Dean Mann, Mary Carrington, Mario Clerici, Gene M. Shearer, David P. Carbone, David R. Scott, Richard A. Houghten, Jay A. Berzofsky

Pediatrics

Research output: Contribution to journal › Article › peer-review

123 Scopus citations

Abstract

Human papillomavirus (HPV) is believed to be the major cause of cervical cancer. To investigate whether a cellular immune response, especially a T helper type 1 response, is related to the natural defense against HPV- related cervical lesions, the interleukin 2 response of peripheral blood lymphocytes in vitro to overlapping peptides from HPV-16 E6 and E7 oncoproteins was compared with the degree of cervical cytological abnormality among 140 women in a cross-sectional study. We compared 66 women diagnosed with low-grade squamous intraepithelial lesions (LSIL), 21 with high-grade squamous intraepithelial lesions (HSIL), and 28 with invasive cervical cancer with 25 women who were cytologically normal but previously HPV-16 DNA positive. The fraction showing strong interleukin 2 production against HPV- 16 peptides was greatest among cytologically normal women (35%) and declined with increasing disease severity [LSIL] (20%), HSIL (17%), and cancer patients (7%); χ² test P for the trend = 0.02], whereas the responses against a recall influenza antigen were not significantly different among groups. Our finding suggests that a T helper lymphocyte type 1 response to HPV antigens is associated with disease status. This result may reflect a targeted effect of the disease on immune function or a protective effect of the immune response against disease progression.

Original language	English (US)
Pages (from-to)	3967-3974
Number of pages	8
Journal	Cancer research
Volume	56
Issue number	17
State	Published - Sep 1 1996

ASJC Scopus subject areas

Oncology
Cancer Research

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Tsukui, T., Hildesheim, A., Schiffman, M. H., Lucci, J., Contois, D., Lawler, P., Rush, B. B., Lorincz, A. T., Corrigan, A., Burk, R. D., Qu, W., Marshall, M. A., Mann, D., Carrington, M., Clerici, M., Shearer, G. M., Carbone, D. P., Scott, D. R., Houghten, R. A., & Berzofsky, J. A. (1996). Interleukin 2 production in vitro by peripheral lymphocytes in response to human papillomavirus-derived peptides: Correlation with cervical pathology. Cancer research, 56(17), 3967-3974.

Tsukui, T, Hildesheim, A, Schiffman, MH, Lucci, J, Contois, D, Lawler, P, Rush, BB, Lorincz, AT, Corrigan, A, Burk, RD, Qu, W, Marshall, MA, Mann, D, Carrington, M, Clerici, M, Shearer, GM, Carbone, DP, Scott, DR, Houghten, RA & Berzofsky, JA 1996, 'Interleukin 2 production in vitro by peripheral lymphocytes in response to human papillomavirus-derived peptides: Correlation with cervical pathology', Cancer research, vol. 56, no. 17, pp. 3967-3974.

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title = "Interleukin 2 production in vitro by peripheral lymphocytes in response to human papillomavirus-derived peptides: Correlation with cervical pathology",

abstract = "Human papillomavirus (HPV) is believed to be the major cause of cervical cancer. To investigate whether a cellular immune response, especially a T helper type 1 response, is related to the natural defense against HPV- related cervical lesions, the interleukin 2 response of peripheral blood lymphocytes in vitro to overlapping peptides from HPV-16 E6 and E7 oncoproteins was compared with the degree of cervical cytological abnormality among 140 women in a cross-sectional study. We compared 66 women diagnosed with low-grade squamous intraepithelial lesions (LSIL), 21 with high-grade squamous intraepithelial lesions (HSIL), and 28 with invasive cervical cancer with 25 women who were cytologically normal but previously HPV-16 DNA positive. The fraction showing strong interleukin 2 production against HPV- 16 peptides was greatest among cytologically normal women (35%) and declined with increasing disease severity [LSIL] (20%), HSIL (17%), and cancer patients (7%); χ2 test P for the trend = 0.02], whereas the responses against a recall influenza antigen were not significantly different among groups. Our finding suggests that a T helper lymphocyte type 1 response to HPV antigens is associated with disease status. This result may reflect a targeted effect of the disease on immune function or a protective effect of the immune response against disease progression.",

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AU - Lucci, Joseph

AU - Contois, David

AU - Lawler, Patricia

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AU - Lorincz, Attila T.

AU - Corrigan, Alice

AU - Burk, Robert D.

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AU - Marshall, Margaret A.

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AU - Carrington, Mary

AU - Clerici, Mario

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N2 - Human papillomavirus (HPV) is believed to be the major cause of cervical cancer. To investigate whether a cellular immune response, especially a T helper type 1 response, is related to the natural defense against HPV- related cervical lesions, the interleukin 2 response of peripheral blood lymphocytes in vitro to overlapping peptides from HPV-16 E6 and E7 oncoproteins was compared with the degree of cervical cytological abnormality among 140 women in a cross-sectional study. We compared 66 women diagnosed with low-grade squamous intraepithelial lesions (LSIL), 21 with high-grade squamous intraepithelial lesions (HSIL), and 28 with invasive cervical cancer with 25 women who were cytologically normal but previously HPV-16 DNA positive. The fraction showing strong interleukin 2 production against HPV- 16 peptides was greatest among cytologically normal women (35%) and declined with increasing disease severity [LSIL] (20%), HSIL (17%), and cancer patients (7%); χ2 test P for the trend = 0.02], whereas the responses against a recall influenza antigen were not significantly different among groups. Our finding suggests that a T helper lymphocyte type 1 response to HPV antigens is associated with disease status. This result may reflect a targeted effect of the disease on immune function or a protective effect of the immune response against disease progression.

AB - Human papillomavirus (HPV) is believed to be the major cause of cervical cancer. To investigate whether a cellular immune response, especially a T helper type 1 response, is related to the natural defense against HPV- related cervical lesions, the interleukin 2 response of peripheral blood lymphocytes in vitro to overlapping peptides from HPV-16 E6 and E7 oncoproteins was compared with the degree of cervical cytological abnormality among 140 women in a cross-sectional study. We compared 66 women diagnosed with low-grade squamous intraepithelial lesions (LSIL), 21 with high-grade squamous intraepithelial lesions (HSIL), and 28 with invasive cervical cancer with 25 women who were cytologically normal but previously HPV-16 DNA positive. The fraction showing strong interleukin 2 production against HPV- 16 peptides was greatest among cytologically normal women (35%) and declined with increasing disease severity [LSIL] (20%), HSIL (17%), and cancer patients (7%); χ2 test P for the trend = 0.02], whereas the responses against a recall influenza antigen were not significantly different among groups. Our finding suggests that a T helper lymphocyte type 1 response to HPV antigens is associated with disease status. This result may reflect a targeted effect of the disease on immune function or a protective effect of the immune response against disease progression.

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Interleukin 2 production in vitro by peripheral lymphocytes in response to human papillomavirus-derived peptides: Correlation with cervical pathology

Abstract

ASJC Scopus subject areas

UN SDGs

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