Interleukin 2 production in vitro by peripheral lymphocytes in response to human papillomavirus-derived peptides: Correlation with cervical pathology

Taku Tsukui, Allan Hildesheim, Mark H. Schiffman, Joseph Lucci, David Contois, Patricia Lawler, Brenda B. Rush, Attila T. Lorincz, Alice Corrigan, Robert D. Burk, Weimin Qu, Margaret A. Marshall, Dean Mann, Mary Carrington, Mario Clerici, Gene M. Shearer, David P. Carbone, David R. Scott, Richard A. Houghten, Jay A. Berzofsky

Research output: Contribution to journalArticle

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Abstract

Human papillomavirus (HPV) is believed to be the major cause of cervical cancer. To investigate whether a cellular immune response, especially a T helper type 1 response, is related to the natural defense against HPV- related cervical lesions, the interleukin 2 response of peripheral blood lymphocytes in vitro to overlapping peptides from HPV-16 E6 and E7 oncoproteins was compared with the degree of cervical cytological abnormality among 140 women in a cross-sectional study. We compared 66 women diagnosed with low-grade squamous intraepithelial lesions (LSIL), 21 with high-grade squamous intraepithelial lesions (HSIL), and 28 with invasive cervical cancer with 25 women who were cytologically normal but previously HPV-16 DNA positive. The fraction showing strong interleukin 2 production against HPV- 16 peptides was greatest among cytologically normal women (35%) and declined with increasing disease severity [LSIL] (20%), HSIL (17%), and cancer patients (7%); χ2 test P for the trend = 0.02], whereas the responses against a recall influenza antigen were not significantly different among groups. Our finding suggests that a T helper lymphocyte type 1 response to HPV antigens is associated with disease status. This result may reflect a targeted effect of the disease on immune function or a protective effect of the immune response against disease progression.

Original languageEnglish (US)
Pages (from-to)3967-3974
Number of pages8
JournalCancer Research
Volume56
Issue number17
StatePublished - Sep 1 1996

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Interleukin-2
Human papillomavirus 16
Lymphocytes
Pathology
Peptides
Uterine Cervical Neoplasms
Antigens
Oncogene Proteins
Immune System Diseases
Helper-Inducer T-Lymphocytes
Cellular Immunity
Human Influenza
Disease Progression
Cross-Sectional Studies
In Vitro Techniques
Squamous Intraepithelial Lesions of the Cervix
DNA
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Tsukui, T., Hildesheim, A., Schiffman, M. H., Lucci, J., Contois, D., Lawler, P., ... Berzofsky, J. A. (1996). Interleukin 2 production in vitro by peripheral lymphocytes in response to human papillomavirus-derived peptides: Correlation with cervical pathology. Cancer Research, 56(17), 3967-3974.

Interleukin 2 production in vitro by peripheral lymphocytes in response to human papillomavirus-derived peptides : Correlation with cervical pathology. / Tsukui, Taku; Hildesheim, Allan; Schiffman, Mark H.; Lucci, Joseph; Contois, David; Lawler, Patricia; Rush, Brenda B.; Lorincz, Attila T.; Corrigan, Alice; Burk, Robert D.; Qu, Weimin; Marshall, Margaret A.; Mann, Dean; Carrington, Mary; Clerici, Mario; Shearer, Gene M.; Carbone, David P.; Scott, David R.; Houghten, Richard A.; Berzofsky, Jay A.

In: Cancer Research, Vol. 56, No. 17, 01.09.1996, p. 3967-3974.

Research output: Contribution to journalArticle

Tsukui, T, Hildesheim, A, Schiffman, MH, Lucci, J, Contois, D, Lawler, P, Rush, BB, Lorincz, AT, Corrigan, A, Burk, RD, Qu, W, Marshall, MA, Mann, D, Carrington, M, Clerici, M, Shearer, GM, Carbone, DP, Scott, DR, Houghten, RA & Berzofsky, JA 1996, 'Interleukin 2 production in vitro by peripheral lymphocytes in response to human papillomavirus-derived peptides: Correlation with cervical pathology', Cancer Research, vol. 56, no. 17, pp. 3967-3974.
Tsukui, Taku ; Hildesheim, Allan ; Schiffman, Mark H. ; Lucci, Joseph ; Contois, David ; Lawler, Patricia ; Rush, Brenda B. ; Lorincz, Attila T. ; Corrigan, Alice ; Burk, Robert D. ; Qu, Weimin ; Marshall, Margaret A. ; Mann, Dean ; Carrington, Mary ; Clerici, Mario ; Shearer, Gene M. ; Carbone, David P. ; Scott, David R. ; Houghten, Richard A. ; Berzofsky, Jay A. / Interleukin 2 production in vitro by peripheral lymphocytes in response to human papillomavirus-derived peptides : Correlation with cervical pathology. In: Cancer Research. 1996 ; Vol. 56, No. 17. pp. 3967-3974.
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