Abstract
Human papillomavirus (HPV) is believed to be the major cause of cervical cancer. To investigate whether a cellular immune response, especially a T helper type 1 response, is related to the natural defense against HPV- related cervical lesions, the interleukin 2 response of peripheral blood lymphocytes in vitro to overlapping peptides from HPV-16 E6 and E7 oncoproteins was compared with the degree of cervical cytological abnormality among 140 women in a cross-sectional study. We compared 66 women diagnosed with low-grade squamous intraepithelial lesions (LSIL), 21 with high-grade squamous intraepithelial lesions (HSIL), and 28 with invasive cervical cancer with 25 women who were cytologically normal but previously HPV-16 DNA positive. The fraction showing strong interleukin 2 production against HPV- 16 peptides was greatest among cytologically normal women (35%) and declined with increasing disease severity [LSIL] (20%), HSIL (17%), and cancer patients (7%); χ2 test P for the trend = 0.02], whereas the responses against a recall influenza antigen were not significantly different among groups. Our finding suggests that a T helper lymphocyte type 1 response to HPV antigens is associated with disease status. This result may reflect a targeted effect of the disease on immune function or a protective effect of the immune response against disease progression.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3967-3974 |
| Number of pages | 8 |
| Journal | Cancer Research |
| Volume | 56 |
| Issue number | 17 |
| State | Published - Sep 1 1996 |
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ASJC Scopus subject areas
- Cancer Research
- Oncology
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Interleukin 2 production in vitro by peripheral lymphocytes in response to human papillomavirus-derived peptides : Correlation with cervical pathology. / Tsukui, Taku; Hildesheim, Allan; Schiffman, Mark H.; Lucci, Joseph; Contois, David; Lawler, Patricia; Rush, Brenda B.; Lorincz, Attila T.; Corrigan, Alice; Burk, Robert D.; Qu, Weimin; Marshall, Margaret A.; Mann, Dean; Carrington, Mary; Clerici, Mario; Shearer, Gene M.; Carbone, David P.; Scott, David R.; Houghten, Richard A.; Berzofsky, Jay A.
In: Cancer Research, Vol. 56, No. 17, 01.09.1996, p. 3967-3974.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Interleukin 2 production in vitro by peripheral lymphocytes in response to human papillomavirus-derived peptides
T2 - Correlation with cervical pathology
AU - Tsukui, Taku
AU - Hildesheim, Allan
AU - Schiffman, Mark H.
AU - Lucci, Joseph
AU - Contois, David
AU - Lawler, Patricia
AU - Rush, Brenda B.
AU - Lorincz, Attila T.
AU - Corrigan, Alice
AU - Burk, Robert D.
AU - Qu, Weimin
AU - Marshall, Margaret A.
AU - Mann, Dean
AU - Carrington, Mary
AU - Clerici, Mario
AU - Shearer, Gene M.
AU - Carbone, David P.
AU - Scott, David R.
AU - Houghten, Richard A.
AU - Berzofsky, Jay A.
PY - 1996/9/1
Y1 - 1996/9/1
N2 - Human papillomavirus (HPV) is believed to be the major cause of cervical cancer. To investigate whether a cellular immune response, especially a T helper type 1 response, is related to the natural defense against HPV- related cervical lesions, the interleukin 2 response of peripheral blood lymphocytes in vitro to overlapping peptides from HPV-16 E6 and E7 oncoproteins was compared with the degree of cervical cytological abnormality among 140 women in a cross-sectional study. We compared 66 women diagnosed with low-grade squamous intraepithelial lesions (LSIL), 21 with high-grade squamous intraepithelial lesions (HSIL), and 28 with invasive cervical cancer with 25 women who were cytologically normal but previously HPV-16 DNA positive. The fraction showing strong interleukin 2 production against HPV- 16 peptides was greatest among cytologically normal women (35%) and declined with increasing disease severity [LSIL] (20%), HSIL (17%), and cancer patients (7%); χ2 test P for the trend = 0.02], whereas the responses against a recall influenza antigen were not significantly different among groups. Our finding suggests that a T helper lymphocyte type 1 response to HPV antigens is associated with disease status. This result may reflect a targeted effect of the disease on immune function or a protective effect of the immune response against disease progression.
AB - Human papillomavirus (HPV) is believed to be the major cause of cervical cancer. To investigate whether a cellular immune response, especially a T helper type 1 response, is related to the natural defense against HPV- related cervical lesions, the interleukin 2 response of peripheral blood lymphocytes in vitro to overlapping peptides from HPV-16 E6 and E7 oncoproteins was compared with the degree of cervical cytological abnormality among 140 women in a cross-sectional study. We compared 66 women diagnosed with low-grade squamous intraepithelial lesions (LSIL), 21 with high-grade squamous intraepithelial lesions (HSIL), and 28 with invasive cervical cancer with 25 women who were cytologically normal but previously HPV-16 DNA positive. The fraction showing strong interleukin 2 production against HPV- 16 peptides was greatest among cytologically normal women (35%) and declined with increasing disease severity [LSIL] (20%), HSIL (17%), and cancer patients (7%); χ2 test P for the trend = 0.02], whereas the responses against a recall influenza antigen were not significantly different among groups. Our finding suggests that a T helper lymphocyte type 1 response to HPV antigens is associated with disease status. This result may reflect a targeted effect of the disease on immune function or a protective effect of the immune response against disease progression.
UR - http://www.scopus.com/inward/record.url?scp=9444268103&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=9444268103&partnerID=8YFLogxK
M3 - Article
C2 - 8752165
AN - SCOPUS:9444268103
VL - 56
SP - 3967
EP - 3974
JO - Cancer Research
JF - Cancer Research
SN - 0008-5472
IS - 17
ER -