Interleukin-2 driven nuclear translocation of prolactin in cloned t-lymphocytes

Charles V. Clevenger, Amy L. Sillman, Michael B. Prystowsky

Research output: Contribution to journalArticle

72 Scopus citations

Abstract

The nuclear translocation of PRL is demonstrated at the immunofluorescence and electron microscopic (EM) levels in interleukin-2 (IL2)-stimulated cloned T-cells and concanavalin A-stimulated splenocytes. This translocation occurs 2-10 h after IL2 stimulation, and is reversed by the addition of anti-PRL antiserum into the extracellular culture medium. The nuclear localization of PRL in IL2 stimulated T-cells was confirmed by postembedding immunogold EM. The nuclear uptake of PRL after IL2 stimulation was further documented by EM studies using PRL-colloidal gold conjugates. These studies suggest that the intranuclear PRL is translocated from the extracellular medium via an endosomal/lysosomal pathway over a period of several hours. Finally, the requirement for PRL no later than 6 h after IL2 stimulation is demonstrated through the reversible inhibition of T-cell growth with anti-PRL antiserum. (Endocrinology 127: 3151-3159, 1990).

Original languageEnglish (US)
Pages (from-to)3151-3159
Number of pages9
JournalEndocrinology
Volume127
Issue number6
DOIs
StatePublished - Dec 1990
Externally publishedYes

ASJC Scopus subject areas

  • Endocrinology

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