Interleukin 1 plus interleukin 3 plus colony-stimulating factor 1 are essential for clonal proliferation of primitive myeloid bone marrow cells

S. H. Bartelmez, T. R. Bradley, I. Bertoncello, D. Y. Mochizuki, R. J. Tushinski, E. R. Stanley, A. J. Hapel, I. G. Young, A. B. Kriegler, G. S. Hodgson

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

The clonal growth in nutrient agar at low cell densities of high-proliferative potential colony-forming cells (HPP-CFC) of bone marrow obtained from mice treated 2 days earlier with 5-fluorouracil (FU) (FU(2d)BM) has been shown to require a combination of three growth factors, interleukin 1 (IL-1), interleukin 3 (IL-3), and macrophage colony-stimulating factor (CSF-1). These HPP-CFC have been enriched 140-fold from FU(2d)BM by fluorescence-activated cell sorting of 7/4-, B220-, and L3T4-negative cells. The mean of the plating efficiencies of these enriched populations was 4.4% and no growth was observed when the factors were used singly. Similarly, enrichments of 16-fold were obtained from FU(2d)BM using immunomagnetic Dynabeads with anti-7/4 plus anti-B220 (meaning plating efficiency 0.5%). The further additions of human granulocyte CSF or mouse granulocyte-macrophage CSF or both to IL-1 plus IL-3 plus CSF-1 did not increase HPP-CFC colony formation, but both augmented the small colony formation with IL-1 plus IL-3, IL-3 plus CSF-1, or IL-1 plus CSF-1.

Original languageEnglish (US)
Pages (from-to)240-245
Number of pages6
JournalExperimental Hematology
Volume17
Issue number3
StatePublished - 1989
Externally publishedYes

Fingerprint

Macrophage Colony-Stimulating Factor
Interleukin-3
Myeloid Cells
Interleukin-1
Bone Marrow Cells
Fluorouracil
Granulocytes
Growth
Agar
Intercellular Signaling Peptides and Proteins
Flow Cytometry
Cell Count
Macrophages
Food
Population

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Bartelmez, S. H., Bradley, T. R., Bertoncello, I., Mochizuki, D. Y., Tushinski, R. J., Stanley, E. R., ... Hodgson, G. S. (1989). Interleukin 1 plus interleukin 3 plus colony-stimulating factor 1 are essential for clonal proliferation of primitive myeloid bone marrow cells. Experimental Hematology, 17(3), 240-245.

Interleukin 1 plus interleukin 3 plus colony-stimulating factor 1 are essential for clonal proliferation of primitive myeloid bone marrow cells. / Bartelmez, S. H.; Bradley, T. R.; Bertoncello, I.; Mochizuki, D. Y.; Tushinski, R. J.; Stanley, E. R.; Hapel, A. J.; Young, I. G.; Kriegler, A. B.; Hodgson, G. S.

In: Experimental Hematology, Vol. 17, No. 3, 1989, p. 240-245.

Research output: Contribution to journalArticle

Bartelmez, SH, Bradley, TR, Bertoncello, I, Mochizuki, DY, Tushinski, RJ, Stanley, ER, Hapel, AJ, Young, IG, Kriegler, AB & Hodgson, GS 1989, 'Interleukin 1 plus interleukin 3 plus colony-stimulating factor 1 are essential for clonal proliferation of primitive myeloid bone marrow cells', Experimental Hematology, vol. 17, no. 3, pp. 240-245.
Bartelmez, S. H. ; Bradley, T. R. ; Bertoncello, I. ; Mochizuki, D. Y. ; Tushinski, R. J. ; Stanley, E. R. ; Hapel, A. J. ; Young, I. G. ; Kriegler, A. B. ; Hodgson, G. S. / Interleukin 1 plus interleukin 3 plus colony-stimulating factor 1 are essential for clonal proliferation of primitive myeloid bone marrow cells. In: Experimental Hematology. 1989 ; Vol. 17, No. 3. pp. 240-245.
@article{18cc68af0528465688d154f898346acc,
title = "Interleukin 1 plus interleukin 3 plus colony-stimulating factor 1 are essential for clonal proliferation of primitive myeloid bone marrow cells",
abstract = "The clonal growth in nutrient agar at low cell densities of high-proliferative potential colony-forming cells (HPP-CFC) of bone marrow obtained from mice treated 2 days earlier with 5-fluorouracil (FU) (FU(2d)BM) has been shown to require a combination of three growth factors, interleukin 1 (IL-1), interleukin 3 (IL-3), and macrophage colony-stimulating factor (CSF-1). These HPP-CFC have been enriched 140-fold from FU(2d)BM by fluorescence-activated cell sorting of 7/4-, B220-, and L3T4-negative cells. The mean of the plating efficiencies of these enriched populations was 4.4{\%} and no growth was observed when the factors were used singly. Similarly, enrichments of 16-fold were obtained from FU(2d)BM using immunomagnetic Dynabeads with anti-7/4 plus anti-B220 (meaning plating efficiency 0.5{\%}). The further additions of human granulocyte CSF or mouse granulocyte-macrophage CSF or both to IL-1 plus IL-3 plus CSF-1 did not increase HPP-CFC colony formation, but both augmented the small colony formation with IL-1 plus IL-3, IL-3 plus CSF-1, or IL-1 plus CSF-1.",
author = "Bartelmez, {S. H.} and Bradley, {T. R.} and I. Bertoncello and Mochizuki, {D. Y.} and Tushinski, {R. J.} and Stanley, {E. R.} and Hapel, {A. J.} and Young, {I. G.} and Kriegler, {A. B.} and Hodgson, {G. S.}",
year = "1989",
language = "English (US)",
volume = "17",
pages = "240--245",
journal = "Experimental Hematology",
issn = "0301-472X",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - Interleukin 1 plus interleukin 3 plus colony-stimulating factor 1 are essential for clonal proliferation of primitive myeloid bone marrow cells

AU - Bartelmez, S. H.

AU - Bradley, T. R.

AU - Bertoncello, I.

AU - Mochizuki, D. Y.

AU - Tushinski, R. J.

AU - Stanley, E. R.

AU - Hapel, A. J.

AU - Young, I. G.

AU - Kriegler, A. B.

AU - Hodgson, G. S.

PY - 1989

Y1 - 1989

N2 - The clonal growth in nutrient agar at low cell densities of high-proliferative potential colony-forming cells (HPP-CFC) of bone marrow obtained from mice treated 2 days earlier with 5-fluorouracil (FU) (FU(2d)BM) has been shown to require a combination of three growth factors, interleukin 1 (IL-1), interleukin 3 (IL-3), and macrophage colony-stimulating factor (CSF-1). These HPP-CFC have been enriched 140-fold from FU(2d)BM by fluorescence-activated cell sorting of 7/4-, B220-, and L3T4-negative cells. The mean of the plating efficiencies of these enriched populations was 4.4% and no growth was observed when the factors were used singly. Similarly, enrichments of 16-fold were obtained from FU(2d)BM using immunomagnetic Dynabeads with anti-7/4 plus anti-B220 (meaning plating efficiency 0.5%). The further additions of human granulocyte CSF or mouse granulocyte-macrophage CSF or both to IL-1 plus IL-3 plus CSF-1 did not increase HPP-CFC colony formation, but both augmented the small colony formation with IL-1 plus IL-3, IL-3 plus CSF-1, or IL-1 plus CSF-1.

AB - The clonal growth in nutrient agar at low cell densities of high-proliferative potential colony-forming cells (HPP-CFC) of bone marrow obtained from mice treated 2 days earlier with 5-fluorouracil (FU) (FU(2d)BM) has been shown to require a combination of three growth factors, interleukin 1 (IL-1), interleukin 3 (IL-3), and macrophage colony-stimulating factor (CSF-1). These HPP-CFC have been enriched 140-fold from FU(2d)BM by fluorescence-activated cell sorting of 7/4-, B220-, and L3T4-negative cells. The mean of the plating efficiencies of these enriched populations was 4.4% and no growth was observed when the factors were used singly. Similarly, enrichments of 16-fold were obtained from FU(2d)BM using immunomagnetic Dynabeads with anti-7/4 plus anti-B220 (meaning plating efficiency 0.5%). The further additions of human granulocyte CSF or mouse granulocyte-macrophage CSF or both to IL-1 plus IL-3 plus CSF-1 did not increase HPP-CFC colony formation, but both augmented the small colony formation with IL-1 plus IL-3, IL-3 plus CSF-1, or IL-1 plus CSF-1.

UR - http://www.scopus.com/inward/record.url?scp=0024599042&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024599042&partnerID=8YFLogxK

M3 - Article

VL - 17

SP - 240

EP - 245

JO - Experimental Hematology

JF - Experimental Hematology

SN - 0301-472X

IS - 3

ER -