Inhibition of Zinc-Dependent Histone Deacetylases with a Chemically Triggered Electrophile

Zarko V. Boskovic, Melissa M. Kemp, Allyson M. Freedy, Vasanthi S. Viswanathan, Marius S. Pop, Jason H. Fuller, Nicole M. Martinez, Samuel O. Figueroa Lazú, Jiyoung A. Hong, Timothy A. Lewis, Daniel Calarese, James D. Love, Amedeo Vetere, Steven C. Almo, Stuart L. Schreiber, Angela N. Koehler

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Unbiased binding assays involving small-molecule microarrays were used to identify compounds that display unique patterns of selectivity among members of the zinc-dependent histone deacetylase family of enzymes. A novel, hydroxyquinoline-containing compound, BRD4354, was shown to preferentially inhibit activity of HDAC5 and HDAC9 in vitro. Inhibition of deacetylase activity appears to be time-dependent and reversible. Mechanistic studies suggest that the compound undergoes zinc-catalyzed decomposition to an ortho-quinone methide, which covalently modifies nucleophilic cysteines within the proteins. The covalent nature of the compound-enzyme interaction has been demonstrated in experiments with biotinylated probe compound and with electrospray ionization-mass spectrometry.

Original languageEnglish (US)
Pages (from-to)1844-1851
Number of pages8
JournalACS Chemical Biology
Volume11
Issue number7
DOIs
StatePublished - Jul 15 2016

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine

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    Boskovic, Z. V., Kemp, M. M., Freedy, A. M., Viswanathan, V. S., Pop, M. S., Fuller, J. H., Martinez, N. M., Figueroa Lazú, S. O., Hong, J. A., Lewis, T. A., Calarese, D., Love, J. D., Vetere, A., Almo, S. C., Schreiber, S. L., & Koehler, A. N. (2016). Inhibition of Zinc-Dependent Histone Deacetylases with a Chemically Triggered Electrophile. ACS Chemical Biology, 11(7), 1844-1851. https://doi.org/10.1021/acschembio.6b00012