Inhibition of SV40 DNA synthesis by camptothecin and neocarzinostatin

E. A. Sausville, Susan Band Horwitz

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Both camptothecin and neocarzinostatin cause 50% inhibition of simian virus 40 DNA synthesis in BSC-1 cells at a drug concentration of 1-0.1 μM. Uniformly prelabeled Form I simian virus 40 DNA is not converted to Form II by 100 μM camptothecin or 10 μM (10 μg/ml) neocarzinostatin, despite extensive drug-induced breakage of cellular DNA at these concentrations. Kinetic experiments examined the fate of replicative intermediates at the onset of inhibition of DNA synthesis. In the presence of 100 μg/ml neocarzinostatin, label proceeds through replicative intermediate molecules and is found largely (> 75%) as Form I simian virus 40 DNA. At 100 μM camptothecin, up to 50% of newly made simian virus 40 DNA is found as a Form II-like species.

Original languageEnglish (US)
Pages (from-to)1156-1166
Number of pages11
JournalMolecular Pharmacology
Volume14
Issue number6
StatePublished - 1978

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Zinostatin
Camptothecin
Simian virus 40
DNA
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Pharmacology

Cite this

Inhibition of SV40 DNA synthesis by camptothecin and neocarzinostatin. / Sausville, E. A.; Band Horwitz, Susan.

In: Molecular Pharmacology, Vol. 14, No. 6, 1978, p. 1156-1166.

Research output: Contribution to journalArticle

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abstract = "Both camptothecin and neocarzinostatin cause 50{\%} inhibition of simian virus 40 DNA synthesis in BSC-1 cells at a drug concentration of 1-0.1 μM. Uniformly prelabeled Form I simian virus 40 DNA is not converted to Form II by 100 μM camptothecin or 10 μM (10 μg/ml) neocarzinostatin, despite extensive drug-induced breakage of cellular DNA at these concentrations. Kinetic experiments examined the fate of replicative intermediates at the onset of inhibition of DNA synthesis. In the presence of 100 μg/ml neocarzinostatin, label proceeds through replicative intermediate molecules and is found largely (> 75{\%}) as Form I simian virus 40 DNA. At 100 μM camptothecin, up to 50{\%} of newly made simian virus 40 DNA is found as a Form II-like species.",
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