Inhibition of oxidative stress and cytokine activity by curcumin in amelioration of endotoxin-induced experimental hepatoxicity in rodents

G. Kaur, N. Tirkey, S. Bharrhan, V. Chanana, P. Rishi, K. Chopra

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

The present study is aimed at investigating the effect of curcumin (CMN) in salvaging endotoxin-induced hepatic dysfunction and oxidative stress in the liver of rodents. Hepatotoxicity was induced by administering lipopolysaccharide (LPS) in a single dose of 1 mg/kg intraperitoneally to the animals, which were being treated with CMN daily for 7 days. Liver enzymes serum alanine aminotransferase (ALT), serum aspartate aminotransferase (AST) and alkaline phosphatase (ALP), total bilirubin and total protein were estimated in serum. Oxidative stress in liver tissue homogenates was estimated by measuring thiobarbituric acid reactive substances (TBARS), glutathione (GSH) content and superoxide dismutase (SOD) activity. Serum and tissue nitrite was estimated using Greiss reagent and served as an indicator of NO production. A separate set of experiments was performed to estimate the effect of CMN on cytokine levels in mouse serum after LPS challenge. LPS induced a marked hepatic dysfunction evident by rise in serum levels of ALT, AST, ALP and total bilirubin (P < 0.05). TBARS levels were significantly increased, whereas GSH and SOD levels decreased in the liver homogenates of LPS-challenged rats. CMN administration attenuated these effects of LPS successfully. Further CMN treatment also regressed various structural changes induced by LPS in the livers of rats and decreased the levels of tumour necrosis factor-α and interleukin-6 in mouse plasma. In conclusion, these findings suggest that CMN attenuates LPS-induced hepatotoxicity possibly by preventing cytotoxic effects of NO, oxygen free radicals and cytokines.

Original languageEnglish (US)
Pages (from-to)313-321
Number of pages9
JournalClinical and Experimental Immunology
Volume145
Issue number2
DOIs
StatePublished - Aug 2006
Externally publishedYes

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Curcumin
Endotoxins
Lipopolysaccharides
Rodentia
Oxidative Stress
Cytokines
Liver
Serum
Thiobarbituric Acid Reactive Substances
Aspartate Aminotransferases
Alanine Transaminase
Bilirubin
Superoxide Dismutase
Alkaline Phosphatase
Nitrites
Free Radicals
Glutathione
Reactive Oxygen Species
Tumor Necrosis Factor-alpha
Enzymes

Keywords

  • Curcumin
  • Free radicals
  • Hepatotoxicity
  • Lipopolysaccharide
  • Nitrosative stress
  • Oxidative stress

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Inhibition of oxidative stress and cytokine activity by curcumin in amelioration of endotoxin-induced experimental hepatoxicity in rodents. / Kaur, G.; Tirkey, N.; Bharrhan, S.; Chanana, V.; Rishi, P.; Chopra, K.

In: Clinical and Experimental Immunology, Vol. 145, No. 2, 08.2006, p. 313-321.

Research output: Contribution to journalArticle

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