Inhibition of HIV-1 virion production by a transdominant mutant of integrase interactor 1

Eric Yung, Masha Sorin, Achintya Pal, Errol Craig, Alexei Morozov, Olivier Delattre, John Kappes, David Ott, Ganjam V. Kalpana

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

Integase interactor 1 (INI1), also known as hSNF5, is a protein that interacts with HIV-1 integrase. We report here that a cytoplasmically localized fragment of INI1 (S6; aa183-294) containing the minimal integrase-interaction domain potently inhibits HIV-1 particle production and replication. Mutations in S6 or integrase that disrupt integrase-INI1 interaction abrogated the inhibitory effect. An integrase-deficient HIV-1 transcomplemented with integrase fused to Vpr was not affected by S6. INI1 was specifically incorporated into virions and was required for efficient HIV-1 particle production. These results indicate that INI1 is required for late events in the viral life cycle, and that ectopic expression of S6 inhibits HIV-1 replication in a transdominant manner via its specific interaction with integrase within the context of Gag-Pol, providing a novel strategy to control HIV-1 replication.

Original languageEnglish (US)
Pages (from-to)920-926
Number of pages7
JournalNature Medicine
Volume7
Issue number8
DOIs
StatePublished - 2001

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Integrases
S 6
Virion
HIV-1
Life Cycle Stages
Life cycle
Mutation
Proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Inhibition of HIV-1 virion production by a transdominant mutant of integrase interactor 1. / Yung, Eric; Sorin, Masha; Pal, Achintya; Craig, Errol; Morozov, Alexei; Delattre, Olivier; Kappes, John; Ott, David; Kalpana, Ganjam V.

In: Nature Medicine, Vol. 7, No. 8, 2001, p. 920-926.

Research output: Contribution to journalArticle

Yung, E, Sorin, M, Pal, A, Craig, E, Morozov, A, Delattre, O, Kappes, J, Ott, D & Kalpana, GV 2001, 'Inhibition of HIV-1 virion production by a transdominant mutant of integrase interactor 1', Nature Medicine, vol. 7, no. 8, pp. 920-926. https://doi.org/10.1038/90959
Yung, Eric ; Sorin, Masha ; Pal, Achintya ; Craig, Errol ; Morozov, Alexei ; Delattre, Olivier ; Kappes, John ; Ott, David ; Kalpana, Ganjam V. / Inhibition of HIV-1 virion production by a transdominant mutant of integrase interactor 1. In: Nature Medicine. 2001 ; Vol. 7, No. 8. pp. 920-926.
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AU - Kappes, John

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