Inhibition of DNA synthesis in living cells by microinjection of G(i2) antibodies

V. J. LaMorte, P. K. Goldsmith, A. M. Spiegel, J. L. Meinkoth, J. R. Feramisco

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

Heterotrimeric guanine nucleotide binding proteins function in the coupling of a diverse span of cell surface receptors to a variety of intracellular signaling pathways, some of which stimulate cellular proliferation. With the recent discovery that mutated forms of G proteins are present in specific tumors, there has been an increased interest in the determination of the role of specific subtypes of G proteins in the regulation of cellular growth. We have attempted to determine which subtypes of G proteins are directly involved in serum-stimulated DNA synthesis through microinjection of inhibitory antibodies into living cells. Inhibitory rabbit polyclonal antibodies directed against specific G(i) α subunits were introduced into living Balb/c 3T3 fibroblasts by microinjection, and the effect upon serum-stimulated DNA synthesis was examined. Results of these experiments indicate that G(i2) plays a direct role in serum-stimulated DNA synthesis in living cells and suggest that G proteins may function in a variety of mitogenic signaling pathways initiated by serum growth factors.

Original languageEnglish (US)
Pages (from-to)691-694
Number of pages4
JournalJournal of Biological Chemistry
Volume267
Issue number2
StatePublished - Jan 1 1992
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    LaMorte, V. J., Goldsmith, P. K., Spiegel, A. M., Meinkoth, J. L., & Feramisco, J. R. (1992). Inhibition of DNA synthesis in living cells by microinjection of G(i2) antibodies. Journal of Biological Chemistry, 267(2), 691-694.