Inhibition of cell proliferation and protease activity by cartilage factors and heparin

Victor Bernard Hatcher, G. Tsien, M. S. Oberman, P. G. Burk

Research output: Chapter in Book/Report/Conference proceedingChapter

13 Citations (Scopus)

Abstract

Proliferating rat smooth muscle cells and fibroblasts have membrane-associated protease activity. High concentrations of heparin inhibited membrane-associated protease activity and cell proliferation, while low concentration of heparin promoted smooth muscle cell proliferation. The inhibition of protease activity and proliferation was abolished when heparin was treated with protamine sulfate or when acid treated fetal calf serum was used. Heparin required the presence of an acid labile factor(s) in serum for the inhibition of protease activity and proliferation. Heparin and antithrombin III in the presence of acid-treated fetal calf serum did not inhibit cell proliferation or protease activity. Cartilage factors isolated from bovine nasal cartilage containing trypsin inhibitory activity, but not papain inhibitory activity, inhibited rat smooth muscle and fibroblast proliferation and surface associated protease activity. The cartilage factors did not require acid-labile components in the fetal calf serum for the inhibitory activity. The inhibitory activity due to heparin and cartilage factors was not permanent under our experimental condition. Protein synthesis was not inhibited by heparin or the cartilage factors. In rat smooth muscle cells and fibroblasts, the expression of surface-associated protease activity was related to the proliferative state of the cells. Surface protease activity was only present on proliferating cells. When surface protease activity was inhibited by high concentrations of heparin in the presence of an acid-labile serum component(s) or cartilage factors cell proliferation was also inhibited.

Original languageEnglish (US)
Title of host publicationJournal of Supramolecular and Cellular Biochemistry
Pages33-46
Number of pages14
Volume14
Edition1
StatePublished - 1980

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Cell proliferation
Cartilage
Heparin
Peptide Hydrolases
Cell Proliferation
Muscle
Fibroblasts
Acids
Smooth Muscle Myocytes
Rats
Serum
Cells
Nasal Cartilages
Membranes
Protamines
Papain
Antithrombin III
Trypsin
Smooth Muscle

ASJC Scopus subject areas

  • Biochemistry

Cite this

Hatcher, V. B., Tsien, G., Oberman, M. S., & Burk, P. G. (1980). Inhibition of cell proliferation and protease activity by cartilage factors and heparin. In Journal of Supramolecular and Cellular Biochemistry (1 ed., Vol. 14, pp. 33-46)

Inhibition of cell proliferation and protease activity by cartilage factors and heparin. / Hatcher, Victor Bernard; Tsien, G.; Oberman, M. S.; Burk, P. G.

Journal of Supramolecular and Cellular Biochemistry. Vol. 14 1. ed. 1980. p. 33-46.

Research output: Chapter in Book/Report/Conference proceedingChapter

Hatcher, VB, Tsien, G, Oberman, MS & Burk, PG 1980, Inhibition of cell proliferation and protease activity by cartilage factors and heparin. in Journal of Supramolecular and Cellular Biochemistry. 1 edn, vol. 14, pp. 33-46.
Hatcher VB, Tsien G, Oberman MS, Burk PG. Inhibition of cell proliferation and protease activity by cartilage factors and heparin. In Journal of Supramolecular and Cellular Biochemistry. 1 ed. Vol. 14. 1980. p. 33-46
Hatcher, Victor Bernard ; Tsien, G. ; Oberman, M. S. ; Burk, P. G. / Inhibition of cell proliferation and protease activity by cartilage factors and heparin. Journal of Supramolecular and Cellular Biochemistry. Vol. 14 1. ed. 1980. pp. 33-46
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