Inhibition of akt activity and calcium channel function coordinately drive cell-cell fusion in the BeWo choriocarcinoma placental cell line

Manu Vatish, Lydia Tesfa, Dimitris Grammatopoulos, Eijiro Yamada, Claire C. Bastie, Jeffrey E. Pessin

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

To establish a simple and quantitative live cell fusion assay for placental syncytialization, we generated stable GFP and dsRed expressing fusogenic BeWo cell lines. Fluorescent Activated Cell Sorting was shown to provide a quantitative determination of forskolin (cAMP-mediated) fusion in a time and concentration dependent manner consistent with the increased secretion of beta human chorionic gonadotrophin (β-HCG) and appearance of multi-nucleated cells. Analyses of the fusion process demonstrated that in addition to increased cAMP levels, simultaneous reduction of intracellular calcium and inhibition of Type 1 phosphatidylinositol 3 kinase (PI3K)/Akt signaling also resulted in cell fusion. Although individual blockade of calcium channel function or PI3K/Akt signaling was without effect, the combination with forskolin resulted in a potentiation of cell fusion. These data demonstrate syncytialization is a complex process that depends upon the regulation of distinct signaling inputs that function in concert with each other.

Original languageEnglish (US)
Article numbere29353
JournalPloS one
Volume7
Issue number1
DOIs
StatePublished - Jan 19 2012

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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