Inhibition of a mitotic motor compromises the formation of dendrite- like processes from neuroblastoma cells

Wenqian Yu, David J. Sharp, Ryoko Kuriyama, Prabhat Mallik, Peter W. Baas

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Microtubules in the axon are uniformly oriented, while microtubules in the dendrite are nonuniformly oriented. We have proposed that these distinct microtubule polarity patterns may arise from a redistribution of molecular motor proteins previously used for mitosis of the developing neuroblast. To address this issue, we performed studies on neuroblastoma cells that undergo mitosis but also generate short processes during interphase. Some of these processes are similar to axons with regard to their morphology and microtubule polarity pattern, while others are similar to dendrites. Treatment with cAMP or retinoic acid inhibits cell division, with the former promoting the development of the axon-like processes and the latter promoting the development of the dendrite-like processes. During mitosis, the kinesin- related motor termed CHO1/MKLP1 is localized within the spindle midzone where it is thought to transport microtubules of opposite orientation relative to one another. During process formation. CHO1/MKLP1 becomes concentrated within the dendrite-like processes but is excluded from the axon-like processes. The levels of CHO1/MKLP1 increase in the presence of retinoic acid but decrease in the presence of cAMP, consistent with a role for the protein in dendritic differentiation. Moreover, treatment of the cultures with antisense oligonucleotides to CHO1/MKLP1 compromises the formation of the dendrite- like processes. We speculate that a redistribution of CHO1/MKLP1 is required for the formation of dendrite-like processes, presumably by establishing their characteristic nonuniform microtubule polarity pattern.

Original languageEnglish (US)
Pages (from-to)659-668
Number of pages10
JournalJournal of Cell Biology
Volume136
Issue number3
DOIs
StatePublished - 1997
Externally publishedYes

Fingerprint

Dendrites
Neuroblastoma
Microtubules
Axons
Mitosis
Tretinoin
Molecular Motor Proteins
Kinesin
Antisense Oligonucleotides
Interphase
Cell Division
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Inhibition of a mitotic motor compromises the formation of dendrite- like processes from neuroblastoma cells. / Yu, Wenqian; Sharp, David J.; Kuriyama, Ryoko; Mallik, Prabhat; Baas, Peter W.

In: Journal of Cell Biology, Vol. 136, No. 3, 1997, p. 659-668.

Research output: Contribution to journalArticle

Yu, Wenqian ; Sharp, David J. ; Kuriyama, Ryoko ; Mallik, Prabhat ; Baas, Peter W. / Inhibition of a mitotic motor compromises the formation of dendrite- like processes from neuroblastoma cells. In: Journal of Cell Biology. 1997 ; Vol. 136, No. 3. pp. 659-668.
@article{7bcbec23665d49e79d7bff51009fa8b3,
title = "Inhibition of a mitotic motor compromises the formation of dendrite- like processes from neuroblastoma cells",
abstract = "Microtubules in the axon are uniformly oriented, while microtubules in the dendrite are nonuniformly oriented. We have proposed that these distinct microtubule polarity patterns may arise from a redistribution of molecular motor proteins previously used for mitosis of the developing neuroblast. To address this issue, we performed studies on neuroblastoma cells that undergo mitosis but also generate short processes during interphase. Some of these processes are similar to axons with regard to their morphology and microtubule polarity pattern, while others are similar to dendrites. Treatment with cAMP or retinoic acid inhibits cell division, with the former promoting the development of the axon-like processes and the latter promoting the development of the dendrite-like processes. During mitosis, the kinesin- related motor termed CHO1/MKLP1 is localized within the spindle midzone where it is thought to transport microtubules of opposite orientation relative to one another. During process formation. CHO1/MKLP1 becomes concentrated within the dendrite-like processes but is excluded from the axon-like processes. The levels of CHO1/MKLP1 increase in the presence of retinoic acid but decrease in the presence of cAMP, consistent with a role for the protein in dendritic differentiation. Moreover, treatment of the cultures with antisense oligonucleotides to CHO1/MKLP1 compromises the formation of the dendrite- like processes. We speculate that a redistribution of CHO1/MKLP1 is required for the formation of dendrite-like processes, presumably by establishing their characteristic nonuniform microtubule polarity pattern.",
author = "Wenqian Yu and Sharp, {David J.} and Ryoko Kuriyama and Prabhat Mallik and Baas, {Peter W.}",
year = "1997",
doi = "10.1083/jcb.136.3.659",
language = "English (US)",
volume = "136",
pages = "659--668",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "3",

}

TY - JOUR

T1 - Inhibition of a mitotic motor compromises the formation of dendrite- like processes from neuroblastoma cells

AU - Yu, Wenqian

AU - Sharp, David J.

AU - Kuriyama, Ryoko

AU - Mallik, Prabhat

AU - Baas, Peter W.

PY - 1997

Y1 - 1997

N2 - Microtubules in the axon are uniformly oriented, while microtubules in the dendrite are nonuniformly oriented. We have proposed that these distinct microtubule polarity patterns may arise from a redistribution of molecular motor proteins previously used for mitosis of the developing neuroblast. To address this issue, we performed studies on neuroblastoma cells that undergo mitosis but also generate short processes during interphase. Some of these processes are similar to axons with regard to their morphology and microtubule polarity pattern, while others are similar to dendrites. Treatment with cAMP or retinoic acid inhibits cell division, with the former promoting the development of the axon-like processes and the latter promoting the development of the dendrite-like processes. During mitosis, the kinesin- related motor termed CHO1/MKLP1 is localized within the spindle midzone where it is thought to transport microtubules of opposite orientation relative to one another. During process formation. CHO1/MKLP1 becomes concentrated within the dendrite-like processes but is excluded from the axon-like processes. The levels of CHO1/MKLP1 increase in the presence of retinoic acid but decrease in the presence of cAMP, consistent with a role for the protein in dendritic differentiation. Moreover, treatment of the cultures with antisense oligonucleotides to CHO1/MKLP1 compromises the formation of the dendrite- like processes. We speculate that a redistribution of CHO1/MKLP1 is required for the formation of dendrite-like processes, presumably by establishing their characteristic nonuniform microtubule polarity pattern.

AB - Microtubules in the axon are uniformly oriented, while microtubules in the dendrite are nonuniformly oriented. We have proposed that these distinct microtubule polarity patterns may arise from a redistribution of molecular motor proteins previously used for mitosis of the developing neuroblast. To address this issue, we performed studies on neuroblastoma cells that undergo mitosis but also generate short processes during interphase. Some of these processes are similar to axons with regard to their morphology and microtubule polarity pattern, while others are similar to dendrites. Treatment with cAMP or retinoic acid inhibits cell division, with the former promoting the development of the axon-like processes and the latter promoting the development of the dendrite-like processes. During mitosis, the kinesin- related motor termed CHO1/MKLP1 is localized within the spindle midzone where it is thought to transport microtubules of opposite orientation relative to one another. During process formation. CHO1/MKLP1 becomes concentrated within the dendrite-like processes but is excluded from the axon-like processes. The levels of CHO1/MKLP1 increase in the presence of retinoic acid but decrease in the presence of cAMP, consistent with a role for the protein in dendritic differentiation. Moreover, treatment of the cultures with antisense oligonucleotides to CHO1/MKLP1 compromises the formation of the dendrite- like processes. We speculate that a redistribution of CHO1/MKLP1 is required for the formation of dendrite-like processes, presumably by establishing their characteristic nonuniform microtubule polarity pattern.

UR - http://www.scopus.com/inward/record.url?scp=0031050564&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031050564&partnerID=8YFLogxK

U2 - 10.1083/jcb.136.3.659

DO - 10.1083/jcb.136.3.659

M3 - Article

C2 - 9024695

AN - SCOPUS:0031050564

VL - 136

SP - 659

EP - 668

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 3

ER -