Influence of fasting status and sample preparation on metabolic biomarker measurements in postmenopausal women

Neil Murphy, Roni T. Falk, Diana B. Messinger, Michael Pollak, Xiaonan (Nan) Xue, Juan Lin, Robin Sgueglia, Howard Strickler, Mia M. Gaudet, Marc J. Gunter

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Epidemiologic data linking metabolic markers-such as insulin, insulin-like growth factors (IGFs)-and adipose tissue-derived factors with cancer are inconsistent. Between-study differences in blood collection protocols, in particular participant's fasting status, may influence measurements. Methods: We investigated the impact of fasting status and blood sample processing time on components of the insulin/IGF axis and in adipokines in a controlled feeding study of 45 healthy postmenopausal-women aged 50-75 years. Fasting blood samples were drawn (T0), after which subjects ate a standardized breakfast; subsequent blood draws were made at 1 hour (T1), 3 hours (T3), and 6 hours (T6) after breakfast. Serum samples were assayed for insulin, C-peptide, total- and free-IGF-I, IGF-binding protein [BP]-1 and -3, total and high molecular weight (HMW)-adiponectin, retinol binding protein-4, plasminogen activator inhibitor (PAI)-1, and resistin. Results: Insulin and C-peptide levels followed similar postprandial trajectories; intra-class correlation coefficients [ICC] for insulin = 0.75, (95%CI:0.64-0.97) and C-peptide (ICC = 0.66, 95% CI:0.54-0.77) were similarly correlated in fasting (Spearman correlation, r = 0.78, 95% CI:0.64-0.88) and postprandial states (T1, r = 0.77 (95%CI: 0.62-0.87); T3,r = 0.78 (95%CI: 0.63-0.87); T6,r = 0.77 (95%CI: 0.61-0.87)). Free-IGF-I and IGFBP-1 levels were also affected by fasting status, whereas total-IGF-I and IGFBP-3 levels remained unchanged. Levels of adipokines were largely insensitive to fasting status and blood sample processing delays. Conclusion: Several components of the insulin/IGF axis were significantly impacted by fasting state and in particular, C-peptide levels were substantially altered postprandially and in a similar manner to insulin.

Original languageEnglish (US)
Article numbere0167832
JournalPLoS One
Volume11
Issue number12
DOIs
StatePublished - Dec 1 2016

Fingerprint

Biomarkers
fasting
Fasting
biomarkers
insulin
Insulin
C-Peptide
Blood
insulin-like growth factor binding proteins
somatomedins
Somatomedins
insulin-like growth factor I
Insulin-Like Growth Factor I
peptides
blood
Insulin-Like Growth Factor Binding Protein 1
adipokines
Insulin-Like Growth Factor Binding Protein 3
Adipokines
Breakfast

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Influence of fasting status and sample preparation on metabolic biomarker measurements in postmenopausal women. / Murphy, Neil; Falk, Roni T.; Messinger, Diana B.; Pollak, Michael; Xue, Xiaonan (Nan); Lin, Juan; Sgueglia, Robin; Strickler, Howard; Gaudet, Mia M.; Gunter, Marc J.

In: PLoS One, Vol. 11, No. 12, e0167832, 01.12.2016.

Research output: Contribution to journalArticle

Murphy, Neil ; Falk, Roni T. ; Messinger, Diana B. ; Pollak, Michael ; Xue, Xiaonan (Nan) ; Lin, Juan ; Sgueglia, Robin ; Strickler, Howard ; Gaudet, Mia M. ; Gunter, Marc J. / Influence of fasting status and sample preparation on metabolic biomarker measurements in postmenopausal women. In: PLoS One. 2016 ; Vol. 11, No. 12.
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abstract = "Background: Epidemiologic data linking metabolic markers-such as insulin, insulin-like growth factors (IGFs)-and adipose tissue-derived factors with cancer are inconsistent. Between-study differences in blood collection protocols, in particular participant's fasting status, may influence measurements. Methods: We investigated the impact of fasting status and blood sample processing time on components of the insulin/IGF axis and in adipokines in a controlled feeding study of 45 healthy postmenopausal-women aged 50-75 years. Fasting blood samples were drawn (T0), after which subjects ate a standardized breakfast; subsequent blood draws were made at 1 hour (T1), 3 hours (T3), and 6 hours (T6) after breakfast. Serum samples were assayed for insulin, C-peptide, total- and free-IGF-I, IGF-binding protein [BP]-1 and -3, total and high molecular weight (HMW)-adiponectin, retinol binding protein-4, plasminogen activator inhibitor (PAI)-1, and resistin. Results: Insulin and C-peptide levels followed similar postprandial trajectories; intra-class correlation coefficients [ICC] for insulin = 0.75, (95{\%}CI:0.64-0.97) and C-peptide (ICC = 0.66, 95{\%} CI:0.54-0.77) were similarly correlated in fasting (Spearman correlation, r = 0.78, 95{\%} CI:0.64-0.88) and postprandial states (T1, r = 0.77 (95{\%}CI: 0.62-0.87); T3,r = 0.78 (95{\%}CI: 0.63-0.87); T6,r = 0.77 (95{\%}CI: 0.61-0.87)). Free-IGF-I and IGFBP-1 levels were also affected by fasting status, whereas total-IGF-I and IGFBP-3 levels remained unchanged. Levels of adipokines were largely insensitive to fasting status and blood sample processing delays. Conclusion: Several components of the insulin/IGF axis were significantly impacted by fasting state and in particular, C-peptide levels were substantially altered postprandially and in a similar manner to insulin.",
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AU - Murphy, Neil

AU - Falk, Roni T.

AU - Messinger, Diana B.

AU - Pollak, Michael

AU - Xue, Xiaonan (Nan)

AU - Lin, Juan

AU - Sgueglia, Robin

AU - Strickler, Howard

AU - Gaudet, Mia M.

AU - Gunter, Marc J.

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N2 - Background: Epidemiologic data linking metabolic markers-such as insulin, insulin-like growth factors (IGFs)-and adipose tissue-derived factors with cancer are inconsistent. Between-study differences in blood collection protocols, in particular participant's fasting status, may influence measurements. Methods: We investigated the impact of fasting status and blood sample processing time on components of the insulin/IGF axis and in adipokines in a controlled feeding study of 45 healthy postmenopausal-women aged 50-75 years. Fasting blood samples were drawn (T0), after which subjects ate a standardized breakfast; subsequent blood draws were made at 1 hour (T1), 3 hours (T3), and 6 hours (T6) after breakfast. Serum samples were assayed for insulin, C-peptide, total- and free-IGF-I, IGF-binding protein [BP]-1 and -3, total and high molecular weight (HMW)-adiponectin, retinol binding protein-4, plasminogen activator inhibitor (PAI)-1, and resistin. Results: Insulin and C-peptide levels followed similar postprandial trajectories; intra-class correlation coefficients [ICC] for insulin = 0.75, (95%CI:0.64-0.97) and C-peptide (ICC = 0.66, 95% CI:0.54-0.77) were similarly correlated in fasting (Spearman correlation, r = 0.78, 95% CI:0.64-0.88) and postprandial states (T1, r = 0.77 (95%CI: 0.62-0.87); T3,r = 0.78 (95%CI: 0.63-0.87); T6,r = 0.77 (95%CI: 0.61-0.87)). Free-IGF-I and IGFBP-1 levels were also affected by fasting status, whereas total-IGF-I and IGFBP-3 levels remained unchanged. Levels of adipokines were largely insensitive to fasting status and blood sample processing delays. Conclusion: Several components of the insulin/IGF axis were significantly impacted by fasting state and in particular, C-peptide levels were substantially altered postprandially and in a similar manner to insulin.

AB - Background: Epidemiologic data linking metabolic markers-such as insulin, insulin-like growth factors (IGFs)-and adipose tissue-derived factors with cancer are inconsistent. Between-study differences in blood collection protocols, in particular participant's fasting status, may influence measurements. Methods: We investigated the impact of fasting status and blood sample processing time on components of the insulin/IGF axis and in adipokines in a controlled feeding study of 45 healthy postmenopausal-women aged 50-75 years. Fasting blood samples were drawn (T0), after which subjects ate a standardized breakfast; subsequent blood draws were made at 1 hour (T1), 3 hours (T3), and 6 hours (T6) after breakfast. Serum samples were assayed for insulin, C-peptide, total- and free-IGF-I, IGF-binding protein [BP]-1 and -3, total and high molecular weight (HMW)-adiponectin, retinol binding protein-4, plasminogen activator inhibitor (PAI)-1, and resistin. Results: Insulin and C-peptide levels followed similar postprandial trajectories; intra-class correlation coefficients [ICC] for insulin = 0.75, (95%CI:0.64-0.97) and C-peptide (ICC = 0.66, 95% CI:0.54-0.77) were similarly correlated in fasting (Spearman correlation, r = 0.78, 95% CI:0.64-0.88) and postprandial states (T1, r = 0.77 (95%CI: 0.62-0.87); T3,r = 0.78 (95%CI: 0.63-0.87); T6,r = 0.77 (95%CI: 0.61-0.87)). Free-IGF-I and IGFBP-1 levels were also affected by fasting status, whereas total-IGF-I and IGFBP-3 levels remained unchanged. Levels of adipokines were largely insensitive to fasting status and blood sample processing delays. Conclusion: Several components of the insulin/IGF axis were significantly impacted by fasting state and in particular, C-peptide levels were substantially altered postprandially and in a similar manner to insulin.

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