Induction of transforming growth factor β-1 in cervical intraepithelial neoplasia in vivo after treatment with β-carotene

John T. Comerci, Carolyn D. Runowicz, Abbie L. Fields, Seymour L. Romney, Prabhudas R. Palan, Anna S. Kadish, Gary L. Goldberg

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Transforming growth factor (TGF) β1 is a potent growth inhibitor of epithelial cells. Loss of responsiveness to TGF-β1 and/or loss of TGF-β1 itself may be important in the progression of cervical intraepithelial neoplasia to invasive cervical cancer. Retinoids have antiproliferative effects on epithelial cells and have been used as chemopreventive and chemotherapeutic agents for several human cancers. There is evidence that retinoids exert their effects by promoting the induction of TGF-β. The aim of this study was to determine whether the expression of TGF-β1 was altered in patients enrolled in a clinical trial designed to test the therapeutic efficacy of β-carotene, a carotenoid metabolized to retinol, in cervical intraepithelial neoplasia. Using an immunohistochemical technique, tissues were stained with two types of antisera that react with the intracellular and extracellular forms of TGF-β1. Matched cervical biopsies taken from 10 patients before and after treatment with β-carotene were immunostained simultaneously to allow direct comparison of relative staining intensity. A significant increase in intracellular TGF-β1 immunoreactivity was noted in cervical epithelial cells in patients with cervical intraepithelial neoplasia after treatment with β-carotene (P = 0.003). These results demonstrate regulation of a TGF-β isoform in vivo in humans in response to β-carotene administered as a chemopreventive agent.

Original languageEnglish (US)
Pages (from-to)157-160
Number of pages4
JournalClinical Cancer Research
Volume3
Issue number2
StatePublished - 1997

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Cervical Intraepithelial Neoplasia
Transforming Growth Factors
Carotenoids
Epithelial Cells
Retinoids
Therapeutics
Growth Inhibitors
Vitamin A
Uterine Cervical Neoplasms
Immune Sera
Protein Isoforms
Clinical Trials
Staining and Labeling
Biopsy

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Comerci, J. T., Runowicz, C. D., Fields, A. L., Romney, S. L., Palan, P. R., Kadish, A. S., & Goldberg, G. L. (1997). Induction of transforming growth factor β-1 in cervical intraepithelial neoplasia in vivo after treatment with β-carotene. Clinical Cancer Research, 3(2), 157-160.

Induction of transforming growth factor β-1 in cervical intraepithelial neoplasia in vivo after treatment with β-carotene. / Comerci, John T.; Runowicz, Carolyn D.; Fields, Abbie L.; Romney, Seymour L.; Palan, Prabhudas R.; Kadish, Anna S.; Goldberg, Gary L.

In: Clinical Cancer Research, Vol. 3, No. 2, 1997, p. 157-160.

Research output: Contribution to journalArticle

Comerci, JT, Runowicz, CD, Fields, AL, Romney, SL, Palan, PR, Kadish, AS & Goldberg, GL 1997, 'Induction of transforming growth factor β-1 in cervical intraepithelial neoplasia in vivo after treatment with β-carotene', Clinical Cancer Research, vol. 3, no. 2, pp. 157-160.
Comerci, John T. ; Runowicz, Carolyn D. ; Fields, Abbie L. ; Romney, Seymour L. ; Palan, Prabhudas R. ; Kadish, Anna S. ; Goldberg, Gary L. / Induction of transforming growth factor β-1 in cervical intraepithelial neoplasia in vivo after treatment with β-carotene. In: Clinical Cancer Research. 1997 ; Vol. 3, No. 2. pp. 157-160.
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