TY - JOUR
T1 - Induction of kidney epithelial morphogenesis by cells expressing wnt-1
AU - Herzlinger, Doris
AU - Qiao, Jizeng
AU - Cohen, David
AU - Ramakrishna, Naren
AU - Brown, Anthony M.C.
PY - 1994/12
Y1 - 1994/12
N2 - During kidney development, unknown signals derived from the ureteric bud induce metanephric mesenchymal cells to differentiate into nephron epithelia. In addition to the ureteric bud, a number of other tissues can act as heterologous inducers of this process in vitro, including embryonic spinal cord. In this report we demonstrate that Wnt-1, a gene that encodes a secreted glycoprotein expressed in embryonic spinal cord, is capable of conferring nephron-inducing activity to fibroblast cell lines. When cocultured with cells expressing exogenous Wnt-1, metanephric mesenchyme differentiated into glomerular and renal tubular epithelia. No such effect was observed using control cells. These data imply that the ability of embryonic spinal cord to act as an inducer of nephrogenesis may result from its production of Wnt-1protein and suggest that a member of the Wnt genefamily may be a mediator of renal epithelial morphogenesis in vivo.
AB - During kidney development, unknown signals derived from the ureteric bud induce metanephric mesenchymal cells to differentiate into nephron epithelia. In addition to the ureteric bud, a number of other tissues can act as heterologous inducers of this process in vitro, including embryonic spinal cord. In this report we demonstrate that Wnt-1, a gene that encodes a secreted glycoprotein expressed in embryonic spinal cord, is capable of conferring nephron-inducing activity to fibroblast cell lines. When cocultured with cells expressing exogenous Wnt-1, metanephric mesenchyme differentiated into glomerular and renal tubular epithelia. No such effect was observed using control cells. These data imply that the ability of embryonic spinal cord to act as an inducer of nephrogenesis may result from its production of Wnt-1protein and suggest that a member of the Wnt genefamily may be a mediator of renal epithelial morphogenesis in vivo.
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U2 - 10.1006/dbio.1994.1360
DO - 10.1006/dbio.1994.1360
M3 - Article
C2 - 7813799
AN - SCOPUS:0028589430
SN - 0012-1606
VL - 166
SP - 815
EP - 818
JO - Developmental Biology
JF - Developmental Biology
IS - 2
ER -