Induction of cell-mediated immune responses to peptide antigens of P. vivax in microparticles using intranasal immunization

Ajaz A. Bhat, Ratnesh K. Seth, Sudhir Kumar, Riyasat Ali, Teena Mohan, Sukla Biswas, D. N. Rao

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

T-cells play a critical role in resistance to malaria, not only because they function as helper cells for an antibody response, but also because they serve as effector cells. Such cellular immunity is directly implicated in protection from sporozoites as well as from blood stage parasites. The aim of this study was to induce cell mediated immune responses to peptide antigens of Plasmodium vivax co-encapsulated with CpG oligodeoxynucleotide (ODN) in microparticles. In the present study, we have investigated the immunomodulatory effects of two CpG adjuvants, CpG 1826 and CpG 2006 to the five peptide antigens of Plasmodium vivax derived from circumsporozoite protein, merozoite surface protein-1, apical membrane antigen-1 and gametocyte surface antigen (Pvs24) in microparticle delivery. The T-cell proliferation response study of the cells collected from spleen, lamina propria and peyer's patches showed significantly high (p<0.001) stimulation index when primed with peptide antigens in microparticles co-encapsulating CpG ODN adjuvant as compared to peptide alone primed mice. The cytokine measurement profile of IFN-γ, TNF-α, IL-2, IL-4 and IL-10 in culture supernatants of cells primed with peptide antigens in microparticles co-encapsulating CpG ODN showed higher levels of IFN- γ followed by TNF-α and IL-2, with relatively low levels of IL-4 and IL-10.

Original languageEnglish (US)
Pages (from-to)483-499
Number of pages17
JournalImmunological Investigations
Volume39
Issue number4-5
DOIs
StatePublished - May 18 2010
Externally publishedYes

Fingerprint

Immunization
Oligodeoxyribonucleotides
Antigens
Peptides
Plasmodium vivax
Interleukin-4
Interleukin-10
Interleukin-2
Merozoite Surface Protein 1
T-Lymphocytes
Sporozoites
Peyer's Patches
Surface Antigens
Helper-Inducer T-Lymphocytes
Cellular Immunity
Malaria
Antibody Formation
Mucous Membrane
Parasites
Spleen

Keywords

  • CD4 cells
  • CpG
  • Cytokines
  • Intranasal immunization
  • Microparticles
  • Peptide

ASJC Scopus subject areas

  • Immunology

Cite this

Induction of cell-mediated immune responses to peptide antigens of P. vivax in microparticles using intranasal immunization. / Bhat, Ajaz A.; Seth, Ratnesh K.; Kumar, Sudhir; Ali, Riyasat; Mohan, Teena; Biswas, Sukla; Rao, D. N.

In: Immunological Investigations, Vol. 39, No. 4-5, 18.05.2010, p. 483-499.

Research output: Contribution to journalArticle

Bhat, Ajaz A. ; Seth, Ratnesh K. ; Kumar, Sudhir ; Ali, Riyasat ; Mohan, Teena ; Biswas, Sukla ; Rao, D. N. / Induction of cell-mediated immune responses to peptide antigens of P. vivax in microparticles using intranasal immunization. In: Immunological Investigations. 2010 ; Vol. 39, No. 4-5. pp. 483-499.
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