Induction by Klebsiella aerogenes of a melanin-like pigment in Cryptococcus neoformans

Susana Frases, Stuart Chaskes, Ekaterina Dadachova, Arturo Casadevall

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

While studying the interaction of Cryptococcus neoformans with Dictyostelium discoideum, we noticed that yeast colonies in agar with a feeder lawn of Klebsiella aerogenes were brown. This finding was intriguing because C. neoformans colonies are not pigmented unless they are provided with precursors for melanization. Strains of all C. neoformans serotypes produced brown pigment in response to K. aerogenes at 22, 30, and 37°C. Pigment production required fungal laccase and was suppressed by high concentrations of glucose. Treatment of brown cells with guanidinium isothiocyanate and hot concentrated HCl yielded participate material that had the physical and chemical characteristics of melanins. No pigment formation was observed when C. neoformans was exposed to live Escherichia coli or heat-killed K. aerogenes. Analysis of K. aerogenes supernatants revealed the presence of dopamine, which can be a substrate for melanin synthesis by C. neoformans. Our findings illustrate a remarkable interaction between a pathogenic fungus and a gram-negative bacterium, in which the bacterium produces a substrate that promotes fungal melanization. This observation provides a precedent that could explain the source of a substrate for C. neoformans melanization in the environment.

Original languageEnglish (US)
Pages (from-to)1542-1550
Number of pages9
JournalApplied and Environmental Microbiology
Volume72
Issue number2
DOIs
StatePublished - Feb 2006

Fingerprint

Klebsiella aerogenes
Enterobacter aerogenes
melanin
Cryptococcus neoformans
Melanins
pigment
pigments
substrate
melanization
bacterium
agar
yeast
glucose
fungus
Dictyostelium discoideum
Laccase
Dictyostelium
laccase
Guanidine
isothiocyanates

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Induction by Klebsiella aerogenes of a melanin-like pigment in Cryptococcus neoformans. / Frases, Susana; Chaskes, Stuart; Dadachova, Ekaterina; Casadevall, Arturo.

In: Applied and Environmental Microbiology, Vol. 72, No. 2, 02.2006, p. 1542-1550.

Research output: Contribution to journalArticle

Frases, Susana ; Chaskes, Stuart ; Dadachova, Ekaterina ; Casadevall, Arturo. / Induction by Klebsiella aerogenes of a melanin-like pigment in Cryptococcus neoformans. In: Applied and Environmental Microbiology. 2006 ; Vol. 72, No. 2. pp. 1542-1550.
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