TY - JOUR
T1 - Induction and disappearance of DNA strand breaks in human peripheral blood lymphocytes and fibroblasts treated with methyl methanesulfonate
AU - Boerrigter, Michael E.T.I.
AU - Mullaart, Erik
AU - Vijg, Jan
N1 - Funding Information:
This work was supported by grants from Senetek p.l.c., the Sandoz Foundation for Gerontological Research, and the Dutch Ministry of Welfare, Health and Cultural Affairs. We thank Drs. F. Berends and G. Douglas for critically reading the manuscript and Mr. M. Boer-mans and Mr. R. van Boven for preparing the figures.
PY - 1991/1
Y1 - 1991/1
N2 - The induction and disappearance of DNA single-strand breaks (SSB) in human peripheral blood lymphocytes (PBL) and fibroblasts exposed to methyl methanesulfonate (MMS) were investigated by using the alkaline filter elution assay. In the two cell types, identical amounts of SSB were induced during a 45-min treatment with a given dose of MMS. In quiescent PBL only 9 ± 4% (mean ± SD) of the induced SSB had disappeared at 1 h after exposure, whereas in phytohemagglutinin-stimulated PBL, 23 ± 12% disappeared within the same repair period. The percentage SSB disappearance in confluent fibroblasts was 25 ± 2% at 1 h after exposure. As in PBL, the percentage SSB disappearance in fibroblasts appeared to be proliferation-dependent; actively dividing fibroblasts removed 50 ± 12% of the MMS-induced SSB during the 1-h repair period. The accumulation of SSB in PBL, but not in fibroblasts, during MMS exposure in the presence of the excision-repair inhibitor 1-β-D-arabinofuranosylcytosine indicated the utilization of different repair pathways in these two cell types. The generally lower rate of disappearance of MMS-induced SSB in PBL as compared to fibroblasts correlated with an increased loss of cell viability, measured by determining the incorporation of [3H]thymidine.
AB - The induction and disappearance of DNA single-strand breaks (SSB) in human peripheral blood lymphocytes (PBL) and fibroblasts exposed to methyl methanesulfonate (MMS) were investigated by using the alkaline filter elution assay. In the two cell types, identical amounts of SSB were induced during a 45-min treatment with a given dose of MMS. In quiescent PBL only 9 ± 4% (mean ± SD) of the induced SSB had disappeared at 1 h after exposure, whereas in phytohemagglutinin-stimulated PBL, 23 ± 12% disappeared within the same repair period. The percentage SSB disappearance in confluent fibroblasts was 25 ± 2% at 1 h after exposure. As in PBL, the percentage SSB disappearance in fibroblasts appeared to be proliferation-dependent; actively dividing fibroblasts removed 50 ± 12% of the MMS-induced SSB during the 1-h repair period. The accumulation of SSB in PBL, but not in fibroblasts, during MMS exposure in the presence of the excision-repair inhibitor 1-β-D-arabinofuranosylcytosine indicated the utilization of different repair pathways in these two cell types. The generally lower rate of disappearance of MMS-induced SSB in PBL as compared to fibroblasts correlated with an increased loss of cell viability, measured by determining the incorporation of [3H]thymidine.
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U2 - 10.1016/0014-4827(91)90157-P
DO - 10.1016/0014-4827(91)90157-P
M3 - Article
C2 - 1984421
AN - SCOPUS:0026024502
SN - 0014-4827
VL - 192
SP - 61
EP - 66
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -