Induced Pluripotent Stem Cells as a Source of Hepatocytes

Vanessa Sauer, Namita Roy-Chowdhury, Chandan Guha, Jayanta Roy-Chowdhury

Research output: Contribution to journalReview article

17 Citations (Scopus)

Abstract

During the past decade, a series of discoveries has established the potential of the so-called terminally differentiated cells to transition to more primitive progenitor cells. The dramatic demonstration of the ability to reprogram differentiated somatic cells to induced pluripotent stem cells (iPSC) that can then give rise to cells of all three germ layers has opened the possibility of generating virtually any cell type in culture, from any given individual. Taking advantage of these concepts, researchers have generated iPSC by reprogramming a wide variety of somatic cells. In addition to their practical implications, these studies have provided crucial insights into the mechanism of cell plasticity that underlies the transition from one cell type to another. Using concepts derived from research on embryological development, investigators have differentiated iPSC to cells resembling hepatocytes in many ways. Such hepatocyte-like cells could be of enormous value in disease modeling, drug discovery and regenerative medicine. However, the currently available methods do not yield cells that fully reproduce the characteristics of adult primary hepatocytes. Thus, generating hepatocytes from iPSC is very much a work in progress. In addition to chronicling these exciting developments, this review will discuss the emergent new approaches to generating iPSC, improving their differentiation to hepatocyte-like cells, and maintaining the hepatocyte-like cells in culture for longer survival and better function.

Original languageEnglish (US)
Pages (from-to)11-20
Number of pages10
JournalCurrent Pathobiology Reports
Volume2
Issue number1
DOIs
StatePublished - Mar 1 2014

Fingerprint

Induced Pluripotent Stem Cells
Hepatocytes
Cell Culture Techniques
Research Personnel
Germ Layers
Regenerative Medicine
Drug Discovery
Stem Cells

Keywords

  • Directed differentiation
  • Hepatocyte-like cells
  • iPS
  • Reprogramming

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Pathology and Forensic Medicine
  • Cancer Research

Cite this

Induced Pluripotent Stem Cells as a Source of Hepatocytes. / Sauer, Vanessa; Roy-Chowdhury, Namita; Guha, Chandan; Roy-Chowdhury, Jayanta.

In: Current Pathobiology Reports, Vol. 2, No. 1, 01.03.2014, p. 11-20.

Research output: Contribution to journalReview article

@article{eee72f3b57ee44968cb0f0486c11a4c1,
title = "Induced Pluripotent Stem Cells as a Source of Hepatocytes",
abstract = "During the past decade, a series of discoveries has established the potential of the so-called terminally differentiated cells to transition to more primitive progenitor cells. The dramatic demonstration of the ability to reprogram differentiated somatic cells to induced pluripotent stem cells (iPSC) that can then give rise to cells of all three germ layers has opened the possibility of generating virtually any cell type in culture, from any given individual. Taking advantage of these concepts, researchers have generated iPSC by reprogramming a wide variety of somatic cells. In addition to their practical implications, these studies have provided crucial insights into the mechanism of cell plasticity that underlies the transition from one cell type to another. Using concepts derived from research on embryological development, investigators have differentiated iPSC to cells resembling hepatocytes in many ways. Such hepatocyte-like cells could be of enormous value in disease modeling, drug discovery and regenerative medicine. However, the currently available methods do not yield cells that fully reproduce the characteristics of adult primary hepatocytes. Thus, generating hepatocytes from iPSC is very much a work in progress. In addition to chronicling these exciting developments, this review will discuss the emergent new approaches to generating iPSC, improving their differentiation to hepatocyte-like cells, and maintaining the hepatocyte-like cells in culture for longer survival and better function.",
keywords = "Directed differentiation, Hepatocyte-like cells, iPS, Reprogramming",
author = "Vanessa Sauer and Namita Roy-Chowdhury and Chandan Guha and Jayanta Roy-Chowdhury",
year = "2014",
month = "3",
day = "1",
doi = "10.1007/s40139-013-0039-2",
language = "English (US)",
volume = "2",
pages = "11--20",
journal = "Current Pathobiology Reports",
issn = "2167-485X",
publisher = "Springer US",
number = "1",

}

TY - JOUR

T1 - Induced Pluripotent Stem Cells as a Source of Hepatocytes

AU - Sauer, Vanessa

AU - Roy-Chowdhury, Namita

AU - Guha, Chandan

AU - Roy-Chowdhury, Jayanta

PY - 2014/3/1

Y1 - 2014/3/1

N2 - During the past decade, a series of discoveries has established the potential of the so-called terminally differentiated cells to transition to more primitive progenitor cells. The dramatic demonstration of the ability to reprogram differentiated somatic cells to induced pluripotent stem cells (iPSC) that can then give rise to cells of all three germ layers has opened the possibility of generating virtually any cell type in culture, from any given individual. Taking advantage of these concepts, researchers have generated iPSC by reprogramming a wide variety of somatic cells. In addition to their practical implications, these studies have provided crucial insights into the mechanism of cell plasticity that underlies the transition from one cell type to another. Using concepts derived from research on embryological development, investigators have differentiated iPSC to cells resembling hepatocytes in many ways. Such hepatocyte-like cells could be of enormous value in disease modeling, drug discovery and regenerative medicine. However, the currently available methods do not yield cells that fully reproduce the characteristics of adult primary hepatocytes. Thus, generating hepatocytes from iPSC is very much a work in progress. In addition to chronicling these exciting developments, this review will discuss the emergent new approaches to generating iPSC, improving their differentiation to hepatocyte-like cells, and maintaining the hepatocyte-like cells in culture for longer survival and better function.

AB - During the past decade, a series of discoveries has established the potential of the so-called terminally differentiated cells to transition to more primitive progenitor cells. The dramatic demonstration of the ability to reprogram differentiated somatic cells to induced pluripotent stem cells (iPSC) that can then give rise to cells of all three germ layers has opened the possibility of generating virtually any cell type in culture, from any given individual. Taking advantage of these concepts, researchers have generated iPSC by reprogramming a wide variety of somatic cells. In addition to their practical implications, these studies have provided crucial insights into the mechanism of cell plasticity that underlies the transition from one cell type to another. Using concepts derived from research on embryological development, investigators have differentiated iPSC to cells resembling hepatocytes in many ways. Such hepatocyte-like cells could be of enormous value in disease modeling, drug discovery and regenerative medicine. However, the currently available methods do not yield cells that fully reproduce the characteristics of adult primary hepatocytes. Thus, generating hepatocytes from iPSC is very much a work in progress. In addition to chronicling these exciting developments, this review will discuss the emergent new approaches to generating iPSC, improving their differentiation to hepatocyte-like cells, and maintaining the hepatocyte-like cells in culture for longer survival and better function.

KW - Directed differentiation

KW - Hepatocyte-like cells

KW - iPS

KW - Reprogramming

UR - http://www.scopus.com/inward/record.url?scp=84941875708&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84941875708&partnerID=8YFLogxK

U2 - 10.1007/s40139-013-0039-2

DO - 10.1007/s40139-013-0039-2

M3 - Review article

AN - SCOPUS:84941875708

VL - 2

SP - 11

EP - 20

JO - Current Pathobiology Reports

JF - Current Pathobiology Reports

SN - 2167-485X

IS - 1

ER -