Independent synthesis of light and heavy chains. Quantitation of light chain production by mouse myeloma variants

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Abstract

The rates of immunoglobulin synthesis were examined in two MPC 11 cell lines which were independently adapted to tissue culture and in light chain producing variants derived from each of them. One cell line synthesized 2.5 pg immunoglobulin/cell/h, while the other synthesized 3.6 pg immunoglobulin/cell/h. The ratio of heavy and light chains in the two cell lines was approximately the same, and the size of the intracellular pool of immunoglobulin was proportioned to the rate of synthesis. Variants which had spontaneously lost the ability to produce heavy chains continued to synthesize light chains at approximately the same rate as their parent cell line.

Original languageEnglish (US)
Pages (from-to)1112-1116
Number of pages5
JournalJournal of Experimental Medicine
Volume140
Issue number4
StatePublished - 1974

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Immunoglobulins
Light
Cell Line

ASJC Scopus subject areas

  • Immunology

Cite this

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title = "Independent synthesis of light and heavy chains. Quantitation of light chain production by mouse myeloma variants",
abstract = "The rates of immunoglobulin synthesis were examined in two MPC 11 cell lines which were independently adapted to tissue culture and in light chain producing variants derived from each of them. One cell line synthesized 2.5 pg immunoglobulin/cell/h, while the other synthesized 3.6 pg immunoglobulin/cell/h. The ratio of heavy and light chains in the two cell lines was approximately the same, and the size of the intracellular pool of immunoglobulin was proportioned to the rate of synthesis. Variants which had spontaneously lost the ability to produce heavy chains continued to synthesize light chains at approximately the same rate as their parent cell line.",
author = "R. Laskov and Scharff, {Matthew D.}",
year = "1974",
language = "English (US)",
volume = "140",
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journal = "Journal of Experimental Medicine",
issn = "0022-1007",
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T1 - Independent synthesis of light and heavy chains. Quantitation of light chain production by mouse myeloma variants

AU - Laskov, R.

AU - Scharff, Matthew D.

PY - 1974

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N2 - The rates of immunoglobulin synthesis were examined in two MPC 11 cell lines which were independently adapted to tissue culture and in light chain producing variants derived from each of them. One cell line synthesized 2.5 pg immunoglobulin/cell/h, while the other synthesized 3.6 pg immunoglobulin/cell/h. The ratio of heavy and light chains in the two cell lines was approximately the same, and the size of the intracellular pool of immunoglobulin was proportioned to the rate of synthesis. Variants which had spontaneously lost the ability to produce heavy chains continued to synthesize light chains at approximately the same rate as their parent cell line.

AB - The rates of immunoglobulin synthesis were examined in two MPC 11 cell lines which were independently adapted to tissue culture and in light chain producing variants derived from each of them. One cell line synthesized 2.5 pg immunoglobulin/cell/h, while the other synthesized 3.6 pg immunoglobulin/cell/h. The ratio of heavy and light chains in the two cell lines was approximately the same, and the size of the intracellular pool of immunoglobulin was proportioned to the rate of synthesis. Variants which had spontaneously lost the ability to produce heavy chains continued to synthesize light chains at approximately the same rate as their parent cell line.

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