Increased urine interleukin-1 levels in aging

Zenghua Liao, John H. Tu, Catherine B. Small, Steven M. Schnipper, David L. Rosenstreich

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Our laboratory previously noted an increase in thymocyte mitogenic activity in the urine of many elderly patients. The present study was performed to verify this finding and to determine if this activity was actually due to an increase in interleukin-1 (IL-1). IL-1 levels were measured in the urine of 33 healthy, ambulatory, elderly subjects (ages 83-95 years), using both a murine thymocyte bioassay, measuring activation by the incorporation of tritiated thymidine and an MTT dye reduction assay. There was a significant increase in urine IL-1 in 85% of elderly individuals. In the MTT dye reduction assay, mean elderly urine IL-1 levels were 0.88 U/ml, in comparison with a young control group (ages 23-37 years) in which urine IL-1 levels were very low (mean IL-1 ≤0.05 U/ml). Urine levels of IL-1 β were also measured by using a sensitive immunoassay (ELISA) and were found to be significantly increased in the elderly (mean = 57.4 pg/ml), compared to the young (mean = 2.5 pg/ml). In contrast, IL-2 levels in urine were very low, with no difference between the young and the elderly. Mean urine protein and creatinine levels did not differ significantly between young and old, and did not account for the increase in urine IL-1 levels. Although its immunologic significance is not yet understood, this striking increase in IL-1 is an unusual and interesting finding that merits further investigation.

Original languageEnglish (US)
Pages (from-to)19-27
Number of pages9
JournalGerontology
Volume39
Issue number1
DOIs
StatePublished - 1993

Fingerprint

Interleukin-1
Urine
Thymocytes
Coloring Agents
Immunoassay
Biological Assay
Thymidine
Interleukin-2
Creatinine
Enzyme-Linked Immunosorbent Assay
Control Groups

Keywords

  • Aging
  • Immunosenescence
  • Interleukin-1

ASJC Scopus subject areas

  • Aging
  • Geriatrics and Gerontology

Cite this

Liao, Z., Tu, J. H., Small, C. B., Schnipper, S. M., & Rosenstreich, D. L. (1993). Increased urine interleukin-1 levels in aging. Gerontology, 39(1), 19-27. https://doi.org/10.1159/000213510

Increased urine interleukin-1 levels in aging. / Liao, Zenghua; Tu, John H.; Small, Catherine B.; Schnipper, Steven M.; Rosenstreich, David L.

In: Gerontology, Vol. 39, No. 1, 1993, p. 19-27.

Research output: Contribution to journalArticle

Liao, Z, Tu, JH, Small, CB, Schnipper, SM & Rosenstreich, DL 1993, 'Increased urine interleukin-1 levels in aging', Gerontology, vol. 39, no. 1, pp. 19-27. https://doi.org/10.1159/000213510
Liao, Zenghua ; Tu, John H. ; Small, Catherine B. ; Schnipper, Steven M. ; Rosenstreich, David L. / Increased urine interleukin-1 levels in aging. In: Gerontology. 1993 ; Vol. 39, No. 1. pp. 19-27.
@article{562e735ee7334541926e0703eb9710d8,
title = "Increased urine interleukin-1 levels in aging",
abstract = "Our laboratory previously noted an increase in thymocyte mitogenic activity in the urine of many elderly patients. The present study was performed to verify this finding and to determine if this activity was actually due to an increase in interleukin-1 (IL-1). IL-1 levels were measured in the urine of 33 healthy, ambulatory, elderly subjects (ages 83-95 years), using both a murine thymocyte bioassay, measuring activation by the incorporation of tritiated thymidine and an MTT dye reduction assay. There was a significant increase in urine IL-1 in 85{\%} of elderly individuals. In the MTT dye reduction assay, mean elderly urine IL-1 levels were 0.88 U/ml, in comparison with a young control group (ages 23-37 years) in which urine IL-1 levels were very low (mean IL-1 ≤0.05 U/ml). Urine levels of IL-1 β were also measured by using a sensitive immunoassay (ELISA) and were found to be significantly increased in the elderly (mean = 57.4 pg/ml), compared to the young (mean = 2.5 pg/ml). In contrast, IL-2 levels in urine were very low, with no difference between the young and the elderly. Mean urine protein and creatinine levels did not differ significantly between young and old, and did not account for the increase in urine IL-1 levels. Although its immunologic significance is not yet understood, this striking increase in IL-1 is an unusual and interesting finding that merits further investigation.",
keywords = "Aging, Immunosenescence, Interleukin-1",
author = "Zenghua Liao and Tu, {John H.} and Small, {Catherine B.} and Schnipper, {Steven M.} and Rosenstreich, {David L.}",
year = "1993",
doi = "10.1159/000213510",
language = "English (US)",
volume = "39",
pages = "19--27",
journal = "Gerontology",
issn = "0304-324X",
publisher = "S. Karger AG",
number = "1",

}

TY - JOUR

T1 - Increased urine interleukin-1 levels in aging

AU - Liao, Zenghua

AU - Tu, John H.

AU - Small, Catherine B.

AU - Schnipper, Steven M.

AU - Rosenstreich, David L.

PY - 1993

Y1 - 1993

N2 - Our laboratory previously noted an increase in thymocyte mitogenic activity in the urine of many elderly patients. The present study was performed to verify this finding and to determine if this activity was actually due to an increase in interleukin-1 (IL-1). IL-1 levels were measured in the urine of 33 healthy, ambulatory, elderly subjects (ages 83-95 years), using both a murine thymocyte bioassay, measuring activation by the incorporation of tritiated thymidine and an MTT dye reduction assay. There was a significant increase in urine IL-1 in 85% of elderly individuals. In the MTT dye reduction assay, mean elderly urine IL-1 levels were 0.88 U/ml, in comparison with a young control group (ages 23-37 years) in which urine IL-1 levels were very low (mean IL-1 ≤0.05 U/ml). Urine levels of IL-1 β were also measured by using a sensitive immunoassay (ELISA) and were found to be significantly increased in the elderly (mean = 57.4 pg/ml), compared to the young (mean = 2.5 pg/ml). In contrast, IL-2 levels in urine were very low, with no difference between the young and the elderly. Mean urine protein and creatinine levels did not differ significantly between young and old, and did not account for the increase in urine IL-1 levels. Although its immunologic significance is not yet understood, this striking increase in IL-1 is an unusual and interesting finding that merits further investigation.

AB - Our laboratory previously noted an increase in thymocyte mitogenic activity in the urine of many elderly patients. The present study was performed to verify this finding and to determine if this activity was actually due to an increase in interleukin-1 (IL-1). IL-1 levels were measured in the urine of 33 healthy, ambulatory, elderly subjects (ages 83-95 years), using both a murine thymocyte bioassay, measuring activation by the incorporation of tritiated thymidine and an MTT dye reduction assay. There was a significant increase in urine IL-1 in 85% of elderly individuals. In the MTT dye reduction assay, mean elderly urine IL-1 levels were 0.88 U/ml, in comparison with a young control group (ages 23-37 years) in which urine IL-1 levels were very low (mean IL-1 ≤0.05 U/ml). Urine levels of IL-1 β were also measured by using a sensitive immunoassay (ELISA) and were found to be significantly increased in the elderly (mean = 57.4 pg/ml), compared to the young (mean = 2.5 pg/ml). In contrast, IL-2 levels in urine were very low, with no difference between the young and the elderly. Mean urine protein and creatinine levels did not differ significantly between young and old, and did not account for the increase in urine IL-1 levels. Although its immunologic significance is not yet understood, this striking increase in IL-1 is an unusual and interesting finding that merits further investigation.

KW - Aging

KW - Immunosenescence

KW - Interleukin-1

UR - http://www.scopus.com/inward/record.url?scp=0027409431&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027409431&partnerID=8YFLogxK

U2 - 10.1159/000213510

DO - 10.1159/000213510

M3 - Article

C2 - 8440487

AN - SCOPUS:0027409431

VL - 39

SP - 19

EP - 27

JO - Gerontology

JF - Gerontology

SN - 0304-324X

IS - 1

ER -