TY - JOUR
T1 - Increased plasma levels of NGAL, a marker of neutrophil activation, in patients with abdominal aortic aneurysm
AU - Ramos-Mozo, Priscila
AU - Madrigal-Matute, Julio
AU - Vega de Ceniga, Melina
AU - Blanco-Colio, Luis Miguel
AU - Meilhac, Olivier
AU - Feldman, Laurent
AU - Michel, Jean Baptiste
AU - Clancy, Paula
AU - Golledge, Jonathan
AU - Norman, Paul E.
AU - Egido, Jesus
AU - Martin-Ventura, José Luis
N1 - Funding Information:
The paper have been supported by grants from the Basque Government (2008111019), cvREMOD 091100, the European Community , FAD project (FP-7, HEALTH F2-2008-200647), the Spanish Ministerio de Ciencia y Tecnología ( SAF 2010/21852 ), HF2007-0120 , Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III, Redes RECAVA ( RD06/0014/0035 ) and EUS2008-03565 . Research conducted by JG is supported by the NHMRC and Office of Health and Medical Research, Australia .
PY - 2012/2
Y1 - 2012/2
N2 - Objective: Neutrophil gelatinase-associated lipocalin (NGAL) plasma concentrations have been associated with cardiovascular diseases. We aimed to assess the association of NGAL with abdominal aortic aneurysm (AAA). Methods: NGAL concentrations were analyzed by Western blotting in conditioned medium of polymorphonuclear neutrophils (PMNs) from AAA patients (n=22) and controls (n=11), and also in aortic biopsies from AAA patients and healthy controls (n=10). Plasma NGAL concentrations were measured by ELISA in three groups of subjects from France (n=60), Spain (n=75) and Australia (n=100) and associated with AAA presence and growth. Results: PMNs isolated from AAA patients secreted significantly greater amounts of NGAL than PMNs from controls. Luminal thrombus released large amounts of NGAL compared to abluminal AAA thrombus, AAA wall and healthy aortic media. Plasma NGAL concentrations were significantly higher in patients with AAA than controls from France [115 (78-200) vs. 94 (72-114). ng/ml, p<0.001]. NGAL plasma concentrations in AAA patients from Spain correlated with other markers of thrombus activity (plasmin-antiplasmin complexes and D-dimer). Furthermore, a positive correlation between plasma NGAL and retrospective AAA growth (rho = 0.4, p=0.01) was observed, which remained significant after adjusting for other risk factors. Plasma NGAL was only weakly associated with prospective growth in both Spanish and Australian patients. Conclusions: NGAL is released by PMNs and by the luminal part of AAA thrombus. NGAL plasma levels were increased in AAA patients compared with healthy subjects and correlated with retrospective AAA growth. Further studies in larger subjects groups are needed to confirm the association between NGAL and AAA presence and growth.
AB - Objective: Neutrophil gelatinase-associated lipocalin (NGAL) plasma concentrations have been associated with cardiovascular diseases. We aimed to assess the association of NGAL with abdominal aortic aneurysm (AAA). Methods: NGAL concentrations were analyzed by Western blotting in conditioned medium of polymorphonuclear neutrophils (PMNs) from AAA patients (n=22) and controls (n=11), and also in aortic biopsies from AAA patients and healthy controls (n=10). Plasma NGAL concentrations were measured by ELISA in three groups of subjects from France (n=60), Spain (n=75) and Australia (n=100) and associated with AAA presence and growth. Results: PMNs isolated from AAA patients secreted significantly greater amounts of NGAL than PMNs from controls. Luminal thrombus released large amounts of NGAL compared to abluminal AAA thrombus, AAA wall and healthy aortic media. Plasma NGAL concentrations were significantly higher in patients with AAA than controls from France [115 (78-200) vs. 94 (72-114). ng/ml, p<0.001]. NGAL plasma concentrations in AAA patients from Spain correlated with other markers of thrombus activity (plasmin-antiplasmin complexes and D-dimer). Furthermore, a positive correlation between plasma NGAL and retrospective AAA growth (rho = 0.4, p=0.01) was observed, which remained significant after adjusting for other risk factors. Plasma NGAL was only weakly associated with prospective growth in both Spanish and Australian patients. Conclusions: NGAL is released by PMNs and by the luminal part of AAA thrombus. NGAL plasma levels were increased in AAA patients compared with healthy subjects and correlated with retrospective AAA growth. Further studies in larger subjects groups are needed to confirm the association between NGAL and AAA presence and growth.
KW - Abdominal aortic aneurysm
KW - Biomarkers
KW - Intraluminal thrombus
KW - Neutrophil gelatinase-associated lipocalin
KW - Polymorphonuclear neutrophils
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U2 - 10.1016/j.atherosclerosis.2011.11.023
DO - 10.1016/j.atherosclerosis.2011.11.023
M3 - Article
C2 - 22169111
AN - SCOPUS:84855917316
SN - 0021-9150
VL - 220
SP - 552
EP - 556
JO - Atherosclerosis
JF - Atherosclerosis
IS - 2
ER -