Increased glucose transporter-1 expression on intermediate monocytes from HIV-infected women with subclinical cardiovascular disease

Tiffany R. Butterfield, David B. Hanna, Robert C. Kaplan, Jorge Kizer, Helen G. Durkin, Mary A. Young, Marek J. Nowicki, Phyllis C. Tien, Elizabeth T. Golub, A. Floris Moore Michelle, Kehmia Titanji, Margaret A. Fischl, Sonya L. Heath, Jefferey Martinson, Suzanne M. Crowe, Clovis S. Palmer, Alan L. Landay, Joshua J. Anzinger

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objective: People living with HIV (PLWH) have chronic immune activation and increased cardiovascular disease (CVD) risk. Activation of monocytes and T lymphocytes causes upregulation of glucose transporter-1 (GLUT1) for efficient function. PLWH have an increased percentage of GLUT1-expressing monocytes and T lymphocytes, but it is unclear if these cells are associated with CVD. We evaluated the expression of GLUT1 and CD38 on monocyte and T lymphocyte populations from HIVinfected women with subclinical CVD. Methods: Participants with more than 75th percentile (n-15) and less than 25th percentile (n=15) age-Adjusted intima-media thickness (IMT) at the right common carotid artery and bifurcation were identified from the Women's Interagency HIV Study. Groups were matched by age, race/ethnicity, smoking status, and CD4 cell count. All women were receiving suppressive antiretroviral therapy except for one high and one low IMT participant. Monocyte and T lymphocyte populations were evaluated for GLUT1 and CD38 expression using flow cytometry. Results: Intermediate monocytes from high IMT women had significantly increased expression of GLUT1 (310 MFI vs. 210 MFI, P=0.024) (66.4% vs. 48.5%, P=0.031) and CD38 (339MFI vs. 211MFI, P=0.002) (10.5%vs. 3.8%, P=0.0002) compared withwomen with lowIMT. High and lowIMT participants showed no differences inGLUT1 or CD38 expression on classical monocytes, nonclassical monocytes, CD4 and CD8 T lymphocytes. Conclusion: GLUT1-expressing intermediate monocytes are elevated in HIV-infected women with subclinical CVD. These cells may contribute to development of CVD in PLWH and could be a novel target to limit inflammation.

Original languageEnglish (US)
Pages (from-to)199-205
Number of pages7
JournalAIDS
Volume31
Issue number2
DOIs
StatePublished - Jan 14 2017

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Facilitative Glucose Transport Proteins
Monocytes
Cardiovascular Diseases
HIV
T-Lymphocytes
Common Carotid Artery
CD4 Lymphocyte Count
Population
Flow Cytometry
Research Design
Up-Regulation
Smoking
Inflammation

Keywords

  • Cardiovascular disease
  • Glut1
  • HIV
  • Monocyte
  • T cell

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

Cite this

Increased glucose transporter-1 expression on intermediate monocytes from HIV-infected women with subclinical cardiovascular disease. / Butterfield, Tiffany R.; Hanna, David B.; Kaplan, Robert C.; Kizer, Jorge; Durkin, Helen G.; Young, Mary A.; Nowicki, Marek J.; Tien, Phyllis C.; Golub, Elizabeth T.; Michelle, A. Floris Moore; Titanji, Kehmia; Fischl, Margaret A.; Heath, Sonya L.; Martinson, Jefferey; Crowe, Suzanne M.; Palmer, Clovis S.; Landay, Alan L.; Anzinger, Joshua J.

In: AIDS, Vol. 31, No. 2, 14.01.2017, p. 199-205.

Research output: Contribution to journalArticle

Butterfield, TR, Hanna, DB, Kaplan, RC, Kizer, J, Durkin, HG, Young, MA, Nowicki, MJ, Tien, PC, Golub, ET, Michelle, AFM, Titanji, K, Fischl, MA, Heath, SL, Martinson, J, Crowe, SM, Palmer, CS, Landay, AL & Anzinger, JJ 2017, 'Increased glucose transporter-1 expression on intermediate monocytes from HIV-infected women with subclinical cardiovascular disease', AIDS, vol. 31, no. 2, pp. 199-205. https://doi.org/10.1097/QAD.0000000000001320
Butterfield, Tiffany R. ; Hanna, David B. ; Kaplan, Robert C. ; Kizer, Jorge ; Durkin, Helen G. ; Young, Mary A. ; Nowicki, Marek J. ; Tien, Phyllis C. ; Golub, Elizabeth T. ; Michelle, A. Floris Moore ; Titanji, Kehmia ; Fischl, Margaret A. ; Heath, Sonya L. ; Martinson, Jefferey ; Crowe, Suzanne M. ; Palmer, Clovis S. ; Landay, Alan L. ; Anzinger, Joshua J. / Increased glucose transporter-1 expression on intermediate monocytes from HIV-infected women with subclinical cardiovascular disease. In: AIDS. 2017 ; Vol. 31, No. 2. pp. 199-205.
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abstract = "Objective: People living with HIV (PLWH) have chronic immune activation and increased cardiovascular disease (CVD) risk. Activation of monocytes and T lymphocytes causes upregulation of glucose transporter-1 (GLUT1) for efficient function. PLWH have an increased percentage of GLUT1-expressing monocytes and T lymphocytes, but it is unclear if these cells are associated with CVD. We evaluated the expression of GLUT1 and CD38 on monocyte and T lymphocyte populations from HIVinfected women with subclinical CVD. Methods: Participants with more than 75th percentile (n-15) and less than 25th percentile (n=15) age-Adjusted intima-media thickness (IMT) at the right common carotid artery and bifurcation were identified from the Women's Interagency HIV Study. Groups were matched by age, race/ethnicity, smoking status, and CD4 cell count. All women were receiving suppressive antiretroviral therapy except for one high and one low IMT participant. Monocyte and T lymphocyte populations were evaluated for GLUT1 and CD38 expression using flow cytometry. Results: Intermediate monocytes from high IMT women had significantly increased expression of GLUT1 (310 MFI vs. 210 MFI, P=0.024) (66.4{\%} vs. 48.5{\%}, P=0.031) and CD38 (339MFI vs. 211MFI, P=0.002) (10.5{\%}vs. 3.8{\%}, P=0.0002) compared withwomen with lowIMT. High and lowIMT participants showed no differences inGLUT1 or CD38 expression on classical monocytes, nonclassical monocytes, CD4 and CD8 T lymphocytes. Conclusion: GLUT1-expressing intermediate monocytes are elevated in HIV-infected women with subclinical CVD. These cells may contribute to development of CVD in PLWH and could be a novel target to limit inflammation.",
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T1 - Increased glucose transporter-1 expression on intermediate monocytes from HIV-infected women with subclinical cardiovascular disease

AU - Butterfield, Tiffany R.

AU - Hanna, David B.

AU - Kaplan, Robert C.

AU - Kizer, Jorge

AU - Durkin, Helen G.

AU - Young, Mary A.

AU - Nowicki, Marek J.

AU - Tien, Phyllis C.

AU - Golub, Elizabeth T.

AU - Michelle, A. Floris Moore

AU - Titanji, Kehmia

AU - Fischl, Margaret A.

AU - Heath, Sonya L.

AU - Martinson, Jefferey

AU - Crowe, Suzanne M.

AU - Palmer, Clovis S.

AU - Landay, Alan L.

AU - Anzinger, Joshua J.

PY - 2017/1/14

Y1 - 2017/1/14

N2 - Objective: People living with HIV (PLWH) have chronic immune activation and increased cardiovascular disease (CVD) risk. Activation of monocytes and T lymphocytes causes upregulation of glucose transporter-1 (GLUT1) for efficient function. PLWH have an increased percentage of GLUT1-expressing monocytes and T lymphocytes, but it is unclear if these cells are associated with CVD. We evaluated the expression of GLUT1 and CD38 on monocyte and T lymphocyte populations from HIVinfected women with subclinical CVD. Methods: Participants with more than 75th percentile (n-15) and less than 25th percentile (n=15) age-Adjusted intima-media thickness (IMT) at the right common carotid artery and bifurcation were identified from the Women's Interagency HIV Study. Groups were matched by age, race/ethnicity, smoking status, and CD4 cell count. All women were receiving suppressive antiretroviral therapy except for one high and one low IMT participant. Monocyte and T lymphocyte populations were evaluated for GLUT1 and CD38 expression using flow cytometry. Results: Intermediate monocytes from high IMT women had significantly increased expression of GLUT1 (310 MFI vs. 210 MFI, P=0.024) (66.4% vs. 48.5%, P=0.031) and CD38 (339MFI vs. 211MFI, P=0.002) (10.5%vs. 3.8%, P=0.0002) compared withwomen with lowIMT. High and lowIMT participants showed no differences inGLUT1 or CD38 expression on classical monocytes, nonclassical monocytes, CD4 and CD8 T lymphocytes. Conclusion: GLUT1-expressing intermediate monocytes are elevated in HIV-infected women with subclinical CVD. These cells may contribute to development of CVD in PLWH and could be a novel target to limit inflammation.

AB - Objective: People living with HIV (PLWH) have chronic immune activation and increased cardiovascular disease (CVD) risk. Activation of monocytes and T lymphocytes causes upregulation of glucose transporter-1 (GLUT1) for efficient function. PLWH have an increased percentage of GLUT1-expressing monocytes and T lymphocytes, but it is unclear if these cells are associated with CVD. We evaluated the expression of GLUT1 and CD38 on monocyte and T lymphocyte populations from HIVinfected women with subclinical CVD. Methods: Participants with more than 75th percentile (n-15) and less than 25th percentile (n=15) age-Adjusted intima-media thickness (IMT) at the right common carotid artery and bifurcation were identified from the Women's Interagency HIV Study. Groups were matched by age, race/ethnicity, smoking status, and CD4 cell count. All women were receiving suppressive antiretroviral therapy except for one high and one low IMT participant. Monocyte and T lymphocyte populations were evaluated for GLUT1 and CD38 expression using flow cytometry. Results: Intermediate monocytes from high IMT women had significantly increased expression of GLUT1 (310 MFI vs. 210 MFI, P=0.024) (66.4% vs. 48.5%, P=0.031) and CD38 (339MFI vs. 211MFI, P=0.002) (10.5%vs. 3.8%, P=0.0002) compared withwomen with lowIMT. High and lowIMT participants showed no differences inGLUT1 or CD38 expression on classical monocytes, nonclassical monocytes, CD4 and CD8 T lymphocytes. Conclusion: GLUT1-expressing intermediate monocytes are elevated in HIV-infected women with subclinical CVD. These cells may contribute to development of CVD in PLWH and could be a novel target to limit inflammation.

KW - Cardiovascular disease

KW - Glut1

KW - HIV

KW - Monocyte

KW - T cell

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