Abstract
Objective: People living with HIV (PLWH) have chronic immune activation and increased cardiovascular disease (CVD) risk. Activation of monocytes and T lymphocytes causes upregulation of glucose transporter-1 (GLUT1) for efficient function. PLWH have an increased percentage of GLUT1-expressing monocytes and T lymphocytes, but it is unclear if these cells are associated with CVD. We evaluated the expression of GLUT1 and CD38 on monocyte and T lymphocyte populations from HIVinfected women with subclinical CVD. Methods: Participants with more than 75th percentile (n-15) and less than 25th percentile (n=15) age-Adjusted intima-media thickness (IMT) at the right common carotid artery and bifurcation were identified from the Women's Interagency HIV Study. Groups were matched by age, race/ethnicity, smoking status, and CD4 cell count. All women were receiving suppressive antiretroviral therapy except for one high and one low IMT participant. Monocyte and T lymphocyte populations were evaluated for GLUT1 and CD38 expression using flow cytometry. Results: Intermediate monocytes from high IMT women had significantly increased expression of GLUT1 (310 MFI vs. 210 MFI, P=0.024) (66.4% vs. 48.5%, P=0.031) and CD38 (339MFI vs. 211MFI, P=0.002) (10.5%vs. 3.8%, P=0.0002) compared withwomen with lowIMT. High and lowIMT participants showed no differences inGLUT1 or CD38 expression on classical monocytes, nonclassical monocytes, CD4 and CD8 T lymphocytes. Conclusion: GLUT1-expressing intermediate monocytes are elevated in HIV-infected women with subclinical CVD. These cells may contribute to development of CVD in PLWH and could be a novel target to limit inflammation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 199-205 |
| Number of pages | 7 |
| Journal | AIDS |
| Volume | 31 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 14 2017 |
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Keywords
- Cardiovascular disease
- Glut1
- HIV
- Monocyte
- T cell
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Infectious Diseases
Cite this
Increased glucose transporter-1 expression on intermediate monocytes from HIV-infected women with subclinical cardiovascular disease. / Butterfield, Tiffany R.; Hanna, David B.; Kaplan, Robert C.; Kizer, Jorge; Durkin, Helen G.; Young, Mary A.; Nowicki, Marek J.; Tien, Phyllis C.; Golub, Elizabeth T.; Michelle, A. Floris Moore; Titanji, Kehmia; Fischl, Margaret A.; Heath, Sonya L.; Martinson, Jefferey; Crowe, Suzanne M.; Palmer, Clovis S.; Landay, Alan L.; Anzinger, Joshua J.
In: AIDS, Vol. 31, No. 2, 14.01.2017, p. 199-205.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Increased glucose transporter-1 expression on intermediate monocytes from HIV-infected women with subclinical cardiovascular disease
AU - Butterfield, Tiffany R.
AU - Hanna, David B.
AU - Kaplan, Robert C.
AU - Kizer, Jorge
AU - Durkin, Helen G.
AU - Young, Mary A.
AU - Nowicki, Marek J.
AU - Tien, Phyllis C.
AU - Golub, Elizabeth T.
AU - Michelle, A. Floris Moore
AU - Titanji, Kehmia
AU - Fischl, Margaret A.
AU - Heath, Sonya L.
AU - Martinson, Jefferey
AU - Crowe, Suzanne M.
AU - Palmer, Clovis S.
AU - Landay, Alan L.
AU - Anzinger, Joshua J.
PY - 2017/1/14
Y1 - 2017/1/14
N2 - Objective: People living with HIV (PLWH) have chronic immune activation and increased cardiovascular disease (CVD) risk. Activation of monocytes and T lymphocytes causes upregulation of glucose transporter-1 (GLUT1) for efficient function. PLWH have an increased percentage of GLUT1-expressing monocytes and T lymphocytes, but it is unclear if these cells are associated with CVD. We evaluated the expression of GLUT1 and CD38 on monocyte and T lymphocyte populations from HIVinfected women with subclinical CVD. Methods: Participants with more than 75th percentile (n-15) and less than 25th percentile (n=15) age-Adjusted intima-media thickness (IMT) at the right common carotid artery and bifurcation were identified from the Women's Interagency HIV Study. Groups were matched by age, race/ethnicity, smoking status, and CD4 cell count. All women were receiving suppressive antiretroviral therapy except for one high and one low IMT participant. Monocyte and T lymphocyte populations were evaluated for GLUT1 and CD38 expression using flow cytometry. Results: Intermediate monocytes from high IMT women had significantly increased expression of GLUT1 (310 MFI vs. 210 MFI, P=0.024) (66.4% vs. 48.5%, P=0.031) and CD38 (339MFI vs. 211MFI, P=0.002) (10.5%vs. 3.8%, P=0.0002) compared withwomen with lowIMT. High and lowIMT participants showed no differences inGLUT1 or CD38 expression on classical monocytes, nonclassical monocytes, CD4 and CD8 T lymphocytes. Conclusion: GLUT1-expressing intermediate monocytes are elevated in HIV-infected women with subclinical CVD. These cells may contribute to development of CVD in PLWH and could be a novel target to limit inflammation.
AB - Objective: People living with HIV (PLWH) have chronic immune activation and increased cardiovascular disease (CVD) risk. Activation of monocytes and T lymphocytes causes upregulation of glucose transporter-1 (GLUT1) for efficient function. PLWH have an increased percentage of GLUT1-expressing monocytes and T lymphocytes, but it is unclear if these cells are associated with CVD. We evaluated the expression of GLUT1 and CD38 on monocyte and T lymphocyte populations from HIVinfected women with subclinical CVD. Methods: Participants with more than 75th percentile (n-15) and less than 25th percentile (n=15) age-Adjusted intima-media thickness (IMT) at the right common carotid artery and bifurcation were identified from the Women's Interagency HIV Study. Groups were matched by age, race/ethnicity, smoking status, and CD4 cell count. All women were receiving suppressive antiretroviral therapy except for one high and one low IMT participant. Monocyte and T lymphocyte populations were evaluated for GLUT1 and CD38 expression using flow cytometry. Results: Intermediate monocytes from high IMT women had significantly increased expression of GLUT1 (310 MFI vs. 210 MFI, P=0.024) (66.4% vs. 48.5%, P=0.031) and CD38 (339MFI vs. 211MFI, P=0.002) (10.5%vs. 3.8%, P=0.0002) compared withwomen with lowIMT. High and lowIMT participants showed no differences inGLUT1 or CD38 expression on classical monocytes, nonclassical monocytes, CD4 and CD8 T lymphocytes. Conclusion: GLUT1-expressing intermediate monocytes are elevated in HIV-infected women with subclinical CVD. These cells may contribute to development of CVD in PLWH and could be a novel target to limit inflammation.
KW - Cardiovascular disease
KW - Glut1
KW - HIV
KW - Monocyte
KW - T cell
UR - http://www.scopus.com/inward/record.url?scp=84994759921&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84994759921&partnerID=8YFLogxK
U2 - 10.1097/QAD.0000000000001320
DO - 10.1097/QAD.0000000000001320
M3 - Article
C2 - 27835618
AN - SCOPUS:84994759921
VL - 31
SP - 199
EP - 205
JO - AIDS
JF - AIDS
SN - 0269-9370
IS - 2
ER -