@article{f87f2428d20342ea8ba00d0174bfa17a,
title = "Increased glucose transporter-1 expression on intermediate monocytes from HIV-infected women with subclinical cardiovascular disease",
abstract = "Objective: People living with HIV (PLWH) have chronic immune activation and increased cardiovascular disease (CVD) risk. Activation of monocytes and T lymphocytes causes upregulation of glucose transporter-1 (GLUT1) for efficient function. PLWH have an increased percentage of GLUT1-expressing monocytes and T lymphocytes, but it is unclear if these cells are associated with CVD. We evaluated the expression of GLUT1 and CD38 on monocyte and T lymphocyte populations from HIVinfected women with subclinical CVD. Methods: Participants with more than 75th percentile (n-15) and less than 25th percentile (n=15) age-Adjusted intima-media thickness (IMT) at the right common carotid artery and bifurcation were identified from the Women's Interagency HIV Study. Groups were matched by age, race/ethnicity, smoking status, and CD4 cell count. All women were receiving suppressive antiretroviral therapy except for one high and one low IMT participant. Monocyte and T lymphocyte populations were evaluated for GLUT1 and CD38 expression using flow cytometry. Results: Intermediate monocytes from high IMT women had significantly increased expression of GLUT1 (310 MFI vs. 210 MFI, P=0.024) (66.4% vs. 48.5%, P=0.031) and CD38 (339MFI vs. 211MFI, P=0.002) (10.5%vs. 3.8%, P=0.0002) compared withwomen with lowIMT. High and lowIMT participants showed no differences inGLUT1 or CD38 expression on classical monocytes, nonclassical monocytes, CD4 and CD8 T lymphocytes. Conclusion: GLUT1-expressing intermediate monocytes are elevated in HIV-infected women with subclinical CVD. These cells may contribute to development of CVD in PLWH and could be a novel target to limit inflammation.",
keywords = "Cardiovascular disease, Glut1, HIV, Monocyte, T cell",
author = "Butterfield, {Tiffany R.} and Hanna, {David B.} and Kaplan, {Robert C.} and Kizer, {Jorge R.} and Durkin, {Helen G.} and Young, {Mary A.} and Nowicki, {Marek J.} and Tien, {Phyllis C.} and Golub, {Elizabeth T.} and Michelle, {A. Floris Moore} and Kehmia Titanji and Fischl, {Margaret A.} and Heath, {Sonya L.} and Jefferey Martinson and Crowe, {Suzanne M.} and Palmer, {Clovis S.} and Landay, {Alan L.} and Anzinger, {Joshua J.}",
note = "Funding Information: The study was supported by the Office of the Principal of the University of the West Indies, Mona and NIH grants U01 AI68636 to the AIDS Clinical Trial Group (A.L.L.) and 1R01HL126543, 1R01HL095140, R21-HL-120394 (R.C.K.). Data in this manuscript were collected by the Women's Interagency HIV Study (WIHS). WIHS (Principal investigators): UAB-MS WIHS (Michael Saag, Mirjam-Colette Kempf, and Deborah Konkle-Parker), U01-AI-103401; Atlanta WIHS (Ighovwerha Ofotokun and Gina Wingood), U01-AI-103408; Bronx WIHS (Kathryn Anastos), U01-AI-035004; Brooklyn WIHS (Howard Minkoff and Deborah Gustafson), U01-AI- 031834; Chicago WIHS (Mardge Cohen and Audrey French), U01-AI-034993; Metropolitan Washington WIHS (Mary Young and Seble Kassaye), U01-AI- 034994; Miami WIHS (M.A.F. and Lisa Metsch), U01- AI-103397; UNC WIHS (Adaora Adimora), U01-AI- 103390; Connie Wofsy Women's HIV Study, Northern California (Ruth Greenblatt, Bradley Aouizerat, and P.C.T.), U01-AI-034989; WIHS Data Management and Analysis Center (Stephen Gange and Elizabeth Golub), U01-AI-042590; Southern California WIHS (Joel Milam), U01-HD-032632 (WIHS I - WIHS IV). The WIHS is funded primarily by the National Institute of Allergy and Infectious Diseases (NIAID), with additional co-funding from the Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), the National Cancer Institute (NCI), the National Institute on Drug Abuse (NIDA), and the National Institute on Mental Health (NIMH). Targeted supplemental funding for specific projects is also provided by the National Institute of Dental and Craniofacial Research (NIDCR), the National Institute on Alcohol Abuse and Alcoholism (NIAAA), the National Institute on Deafness and other Communication Disorders (NIDCD), and the NIH Office of Research on Women's Health. WIHS data collection is also supported by UL1-TR000004 (UCSF CTSA) and UL1-TR000454 (Atlanta CTSA). Publisher Copyright: Copyright {\textcopyright} 2017 Wolters Kluwer Health, Inc. All rights reserved.",
year = "2017",
month = jan,
day = "14",
doi = "10.1097/QAD.0000000000001320",
language = "English (US)",
volume = "31",
pages = "199--205",
journal = "AIDS",
issn = "0269-9370",
publisher = "Lippincott Williams and Wilkins",
number = "2",
}