In vivo magnetic resonance imaging of fetal cat neural tissue transplants in the adult cat spinal cord

E. D. Wirth, D. P. Theele, T. H. Mareci, D. K. Anderson, S. A. Brown, P. J. Reier

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Magnetic resonance (MR) imaging was evaluated for its possible diagnostic application in determining the survival of fetal central nervous system tissue grafts in the injured spinal cord. Hemisection cavities were made at the T11-L1 level of eight adult female cats. Immediately thereafter, several pieces of tissue, either obtained from the fetal cat brain stem on embryonic Day 37 (E-37), from the fetal neocortex on E-37, or from the fetal spinal cord on E-23, were implanted into the cavities made in seven cats. The eight cat served as a control for the effect of the lesion only. In another group of four animals, a static-load compression injury was made at the L-2 level. Seven weeks later, the lesion was resected in three cases and fragments of either fetal brainstem or spinal cord tissue were introduced. A small cyst was observed in a fourth cat in the compression injury group and a suspension of dissociated E-23 brain-stem cells was injected into this region of cavitation without disturbing the surrounding leptomeninges. Five months to 2 years posttransplantation, MR imaging was performed with a 2.0-tesla VIS imaging spectrometer by acquiring multislice spin-echo images (TR 1000 msec, TE 30 msec) in both the transverse and sagittal planes. Collectively, these intermediate-weighted images revealed homogeneous, slightly hyperintense signals at the graft site relative to the neighboring host tissue in seven of the 11 graft recipients. Two of the remaining four cats exhibited signals from the graft site that were approximately isointense with the adjacent host spinal cord, and the final two cats and the lesion-only control presented with very hypointense transplant/resection regions. The hyperintense and isointense images were tentatively interpreted as representing viable graft tissue, whereas the hypointense transplant/resection sites were considered to be indicative of a lack of transplant survival or the absence of tissue in the lesion-only control animal. Postmortem gross inspection of fixed specimens and light microscopy verified the MR findings in the control animal in 10 of the 11 graft recipients by showing either transplants and/or cysts corresponding to the MR images obtained. In one cat in the hemisection group, histological analysis revealed a very small piece of graft tissue that was not detected on the MR images. Therefore, it is suggested that within certain spatial- and contrast-resolution limits, MR imaging can reliably detect the presence of transplanted neural tissue in both the hemisected and compression-injured spinal cord of living animals. Thus, MR imaging can serve as an important adjunct to histological, electrophysiological, and long-term behavioral analyses of graft-mediated anatomical and functional repair of the injured spinal cord. It is further suggested that this noninvasive diagnostic approach offers many advantages in terms of the judicious and optimum use of valuable animal models, and that these findings address an important prerequisite (in situ verification of transplant survival) for any future clinical trials involving these or equivalent neural tissue grafting approaches, when such are warranted.

Original languageEnglish (US)
Pages (from-to)261-274
Number of pages14
JournalJournal of Neurosurgery
Volume76
Issue number2
StatePublished - 1992
Externally publishedYes

Fingerprint

Spinal Cord
Cats
Magnetic Resonance Imaging
Transplants
Brain Stem
Magnetic Resonance Spectroscopy
Cysts
Spinal Cord Regeneration
Tissue Transplantation
Nerve Tissue
Spinal Cord Compression
Neocortex
Wounds and Injuries
Microscopy
Suspensions
Stem Cells
Central Nervous System
Animal Models
Clinical Trials
Light

Keywords

  • cat
  • magnetic resonance imaging
  • spinal cord injury
  • tissue transplantation

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Wirth, E. D., Theele, D. P., Mareci, T. H., Anderson, D. K., Brown, S. A., & Reier, P. J. (1992). In vivo magnetic resonance imaging of fetal cat neural tissue transplants in the adult cat spinal cord. Journal of Neurosurgery, 76(2), 261-274.

In vivo magnetic resonance imaging of fetal cat neural tissue transplants in the adult cat spinal cord. / Wirth, E. D.; Theele, D. P.; Mareci, T. H.; Anderson, D. K.; Brown, S. A.; Reier, P. J.

In: Journal of Neurosurgery, Vol. 76, No. 2, 1992, p. 261-274.

Research output: Contribution to journalArticle

Wirth, ED, Theele, DP, Mareci, TH, Anderson, DK, Brown, SA & Reier, PJ 1992, 'In vivo magnetic resonance imaging of fetal cat neural tissue transplants in the adult cat spinal cord', Journal of Neurosurgery, vol. 76, no. 2, pp. 261-274.
Wirth ED, Theele DP, Mareci TH, Anderson DK, Brown SA, Reier PJ. In vivo magnetic resonance imaging of fetal cat neural tissue transplants in the adult cat spinal cord. Journal of Neurosurgery. 1992;76(2):261-274.
Wirth, E. D. ; Theele, D. P. ; Mareci, T. H. ; Anderson, D. K. ; Brown, S. A. ; Reier, P. J. / In vivo magnetic resonance imaging of fetal cat neural tissue transplants in the adult cat spinal cord. In: Journal of Neurosurgery. 1992 ; Vol. 76, No. 2. pp. 261-274.
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abstract = "Magnetic resonance (MR) imaging was evaluated for its possible diagnostic application in determining the survival of fetal central nervous system tissue grafts in the injured spinal cord. Hemisection cavities were made at the T11-L1 level of eight adult female cats. Immediately thereafter, several pieces of tissue, either obtained from the fetal cat brain stem on embryonic Day 37 (E-37), from the fetal neocortex on E-37, or from the fetal spinal cord on E-23, were implanted into the cavities made in seven cats. The eight cat served as a control for the effect of the lesion only. In another group of four animals, a static-load compression injury was made at the L-2 level. Seven weeks later, the lesion was resected in three cases and fragments of either fetal brainstem or spinal cord tissue were introduced. A small cyst was observed in a fourth cat in the compression injury group and a suspension of dissociated E-23 brain-stem cells was injected into this region of cavitation without disturbing the surrounding leptomeninges. Five months to 2 years posttransplantation, MR imaging was performed with a 2.0-tesla VIS imaging spectrometer by acquiring multislice spin-echo images (TR 1000 msec, TE 30 msec) in both the transverse and sagittal planes. Collectively, these intermediate-weighted images revealed homogeneous, slightly hyperintense signals at the graft site relative to the neighboring host tissue in seven of the 11 graft recipients. Two of the remaining four cats exhibited signals from the graft site that were approximately isointense with the adjacent host spinal cord, and the final two cats and the lesion-only control presented with very hypointense transplant/resection regions. The hyperintense and isointense images were tentatively interpreted as representing viable graft tissue, whereas the hypointense transplant/resection sites were considered to be indicative of a lack of transplant survival or the absence of tissue in the lesion-only control animal. Postmortem gross inspection of fixed specimens and light microscopy verified the MR findings in the control animal in 10 of the 11 graft recipients by showing either transplants and/or cysts corresponding to the MR images obtained. In one cat in the hemisection group, histological analysis revealed a very small piece of graft tissue that was not detected on the MR images. Therefore, it is suggested that within certain spatial- and contrast-resolution limits, MR imaging can reliably detect the presence of transplanted neural tissue in both the hemisected and compression-injured spinal cord of living animals. Thus, MR imaging can serve as an important adjunct to histological, electrophysiological, and long-term behavioral analyses of graft-mediated anatomical and functional repair of the injured spinal cord. It is further suggested that this noninvasive diagnostic approach offers many advantages in terms of the judicious and optimum use of valuable animal models, and that these findings address an important prerequisite (in situ verification of transplant survival) for any future clinical trials involving these or equivalent neural tissue grafting approaches, when such are warranted.",
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