In vivo and in vitro studies of the role of lyophilised blond Lager beer and some bioactive components in the modulation of degenerative processes

Tania Merinas-Amo, Inmaculada Tasset, Antonio M. Díaz-Carretero, Ángeles Alonso-Moraga, Fernando Calahorro

Research output: Contribution to journalArticle

5 Scopus citations


The aim of the present study was to examine the nutraceutic potential of lyophilised blond Lager beer, as well as two of its bioactive components: xanthohumol and folic acid. Several toxicity, antitoxicity, genotoxicity, antigenotoxicity and longevity endpoints were checked in the SMART in vivo Drosophila system. Cytotoxicity in HL-60 promyelocytic and NIH3T3 mouse fibroblasts cells, proapototic DNA fragmentation, comet assay, macroautophagy activity and methylation status were tested in in vitro assays. Lyophilised blond Lager beer could be proposed as a substance with an important nutraceutical value, because of its hopeful results as a lifespan promoter, DNA protection against free radicals in the animal model and its chemopreventive activity in HL-60 cells and enhacer of macroautophagy at moderate doses in NIH3T3 inmortal cells. Only xanthohumol mimics the biological activities found in lyophilised beer.

Original languageEnglish (US)
Pages (from-to)274-294
Number of pages21
JournalJournal of Functional Foods
StatePublished - Dec 1 2016
Externally publishedYes



  • Antigenotoxicity
  • Beer
  • Cytotoxicity
  • Drosophila melanogaster
  • Macroautophagy
  • Tumour cells

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science
  • Nutrition and Dietetics

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