TY - JOUR
T1 - In vivo and in vitro studies of the role of lyophilised blond Lager beer and some bioactive components in the modulation of degenerative processes
AU - Merinas-Amo, Tania
AU - Tasset-Cuevas, Inmaculada
AU - Díaz-Carretero, Antonio M.
AU - Alonso-Moraga, Ángeles
AU - Calahorro, Fernando
N1 - Publisher Copyright:
© 2016 Elsevier Ltd
PY - 2016/12/1
Y1 - 2016/12/1
N2 - The aim of the present study was to examine the nutraceutic potential of lyophilised blond Lager beer, as well as two of its bioactive components: xanthohumol and folic acid. Several toxicity, antitoxicity, genotoxicity, antigenotoxicity and longevity endpoints were checked in the SMART in vivo Drosophila system. Cytotoxicity in HL-60 promyelocytic and NIH3T3 mouse fibroblasts cells, proapototic DNA fragmentation, comet assay, macroautophagy activity and methylation status were tested in in vitro assays. Lyophilised blond Lager beer could be proposed as a substance with an important nutraceutical value, because of its hopeful results as a lifespan promoter, DNA protection against free radicals in the animal model and its chemopreventive activity in HL-60 cells and enhacer of macroautophagy at moderate doses in NIH3T3 inmortal cells. Only xanthohumol mimics the biological activities found in lyophilised beer.
AB - The aim of the present study was to examine the nutraceutic potential of lyophilised blond Lager beer, as well as two of its bioactive components: xanthohumol and folic acid. Several toxicity, antitoxicity, genotoxicity, antigenotoxicity and longevity endpoints were checked in the SMART in vivo Drosophila system. Cytotoxicity in HL-60 promyelocytic and NIH3T3 mouse fibroblasts cells, proapototic DNA fragmentation, comet assay, macroautophagy activity and methylation status were tested in in vitro assays. Lyophilised blond Lager beer could be proposed as a substance with an important nutraceutical value, because of its hopeful results as a lifespan promoter, DNA protection against free radicals in the animal model and its chemopreventive activity in HL-60 cells and enhacer of macroautophagy at moderate doses in NIH3T3 inmortal cells. Only xanthohumol mimics the biological activities found in lyophilised beer.
KW - Antigenotoxicity
KW - Beer
KW - Cytotoxicity
KW - Drosophila melanogaster
KW - Macroautophagy
KW - Tumour cells
UR - http://www.scopus.com/inward/record.url?scp=84988977112&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84988977112&partnerID=8YFLogxK
U2 - 10.1016/j.jff.2016.09.014
DO - 10.1016/j.jff.2016.09.014
M3 - Article
AN - SCOPUS:84988977112
SN - 1756-4646
VL - 27
SP - 274
EP - 294
JO - Journal of Functional Foods
JF - Journal of Functional Foods
ER -