In vitro selection of a deoxyribozyme that can utilize multiple substrates

Matthew Levy, Andrew D. Ellington

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Deoxyribozymes that could catalyze the formation of an internucleotide phosphorothioester linkage were selected from a random sequence pool. During the course of the selection, the pool was successively challenged with five oligonucleotide substrates, each of which terminated in the same hexanucleotide sequence. Selected deoxyribozyme ligases could use all five substrates, albeit to different degrees, and appeared to form, secondary structures that allow differential pairing between the deoxyribozyme and each substrate. These results suggest that early replicases may have been able to bind a variety of oligonucleotide substrates while catalyzing ligation via a common junction.

Original languageEnglish (US)
Pages (from-to)180-190
Number of pages11
JournalJournal of Molecular Evolution
Volume54
Issue number2
DOIs
Publication statusPublished - Jan 29 2002

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Keywords

  • Deoxyribozyme
  • In vitro selection
  • Ligase
  • Nucleic acid replicator
  • Origins of life

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

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