In vitro recoating of reovirus cores with baculovirus-expressed outer- capsid proteins μ1 and σ3

Kartik Chandran, Stephen B. Walker, Ya Chen, Carlo M. Contreras, Leslie A. Schiff, Timothy S. Baker, Max L. Nibert

Research output: Contribution to journalArticle

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Abstract

Reovirus outer-capsid proteins μ1, σ3, and σ1 are thought to be assembled onto nascent core-like particles within infected cells, leading to the production of progeny virions. Consistent with this model, we report the in vitro assembly of baculovirus-expressed μ1 and σ3 onto purified cores that lack μ1, σ3, and σ1. The resulting particles (recoated cores, or r- cores) closely resembled native virions in protein composition (except for lacking cell attachment protein σ1), buoyant density, and particle morphology by scanning cryoelectron microscopy. Transmission cryoelectron microscopy and image reconstruction of r-cores confirmed that they closely resembled virions in the structure of the outer capsid and revealed that assembly of μ1 and σ3 onto cores had induced rearrangement of the pentameric λ2 turrets into a conformation approximating that in virions. r- cores, like virions, underwent proteolytic conversion to particles resembling native ISVPs (infectious subvirion particles) in protein composition, particle morphology, and capacity to permeabilize membranes in vitro. r- cores were 250- to 500-fold more infectious than cores in murine L cells and, like virions but not ISVPs or cores, were inhibited from productively infecting these cells by the presence of either NH4Cl or E-64. The latter results suggest that r-cores and virions used similar routes of entry into L cells, including processing by lysosomal cysteine proteinases, even though the former particles lacked the σ1 protein. To examine the utility of r- cores for genetic dissections of μ1 functions in reovirus entry, we generated r-cores containing a mutant form of μ1 that had been engineered to resist cleavage at the δ:φ junction during conversion to ISVP-like particles by chymotrypsin in vitro. Despite their deficit in δ:φ cleavage, these ISVP-like particles were fully competent to permeabilize membranes in vitro and to infect L cells in the presence of NH4Cl, providing new evidence that this cleavage is dispensable for productive infection.

Original languageEnglish (US)
Pages (from-to)3941-3950
Number of pages10
JournalJournal of Virology
Volume73
Issue number5
StatePublished - 1999
Externally publishedYes

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Reoviridae
Baculoviridae
Capsid Proteins
coat proteins
Virion
virion
Cryoelectron Microscopy
Proteins
protein composition
cells
Computer-Assisted Image Processing
Membranes
Cysteine Proteases
Capsid
Chymotrypsin
In Vitro Techniques
Dissection
capsid
cysteine proteinases
chymotrypsin

ASJC Scopus subject areas

  • Immunology

Cite this

Chandran, K., Walker, S. B., Chen, Y., Contreras, C. M., Schiff, L. A., Baker, T. S., & Nibert, M. L. (1999). In vitro recoating of reovirus cores with baculovirus-expressed outer- capsid proteins μ1 and σ3. Journal of Virology, 73(5), 3941-3950.

In vitro recoating of reovirus cores with baculovirus-expressed outer- capsid proteins μ1 and σ3. / Chandran, Kartik; Walker, Stephen B.; Chen, Ya; Contreras, Carlo M.; Schiff, Leslie A.; Baker, Timothy S.; Nibert, Max L.

In: Journal of Virology, Vol. 73, No. 5, 1999, p. 3941-3950.

Research output: Contribution to journalArticle

Chandran, K, Walker, SB, Chen, Y, Contreras, CM, Schiff, LA, Baker, TS & Nibert, ML 1999, 'In vitro recoating of reovirus cores with baculovirus-expressed outer- capsid proteins μ1 and σ3', Journal of Virology, vol. 73, no. 5, pp. 3941-3950.
Chandran K, Walker SB, Chen Y, Contreras CM, Schiff LA, Baker TS et al. In vitro recoating of reovirus cores with baculovirus-expressed outer- capsid proteins μ1 and σ3. Journal of Virology. 1999;73(5):3941-3950.
Chandran, Kartik ; Walker, Stephen B. ; Chen, Ya ; Contreras, Carlo M. ; Schiff, Leslie A. ; Baker, Timothy S. ; Nibert, Max L. / In vitro recoating of reovirus cores with baculovirus-expressed outer- capsid proteins μ1 and σ3. In: Journal of Virology. 1999 ; Vol. 73, No. 5. pp. 3941-3950.
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