In vitro measurement of rabbit corneal epithelial thickness using ultrahigh resolution optical coherence tomography

Bibiana J. Reiser, Teresa S. Ignacio, Yimin Wang, Mehran Taban, Jordan M. Graff, Paula Sweet, Zhongping Chen, Roy S. Chuck

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

The objective of this study was to reproducibly measure corneal epithelial thickness centrally and at the limbus in the rabbit cornea using ultrahigh resolution optical coherence tomography (OCT). Twelve freshly enucleated New Zealand white rabbit eyes were kept in a moist chamber at 4°C. An ultrahigh resolution OCT system with a spatial resolution of 1.3 μm was used to image the cornea and its component layers. The central and peripheral (limbal) regions of all the samples were scanned within 6 h of harvest in order to minimize the post-mortem degradation of the corneal epithelium. The thickness of the corneal epithelium was determined by measuring the pixel equivalents of the obtained image. Unpaired Student's t-test was used to evaluate differences. The epithelial thickness centrally was found to be 45.8 ± 2.2 μm, and 37.6 ± 1.4 μm at the limbus (P < 0.001). Rabbit corneal epithelium is thicker centrally than at the limbus when measured by ultrahigh resolution OCT. This technique will aid in delineating the pathophysiology of diseases of the anterior cornea.

Original languageEnglish (US)
Pages (from-to)85-88
Number of pages4
JournalVeterinary Ophthalmology
Volume8
Issue number2
DOIs
Publication statusPublished - Apr 14 2005
Externally publishedYes

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Keywords

  • Cornea
  • Epithelial thickness
  • Optical coherence tomography
  • Rabbit

ASJC Scopus subject areas

  • veterinary(all)

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