In vitro and in vivo association of transforming growth factor-β1 with hepatic fibrosis

M. J. Czaja, F. R. Weiner, K. C. Flanders, M. A. Giambrone, R. Wind, L. Biempica, M. A. Zern

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Abstract

Despite extensive efforts, little progress has been made in identifying the factors that induce hepatic fibrosis. Transforming growth factor-β (TGF-β) has been shown to enhance collagen production, therefore its role in hepatic fibrosis was investigated. Treatment of cultured hepatic cells with TGF-β1 increased type I procollagen mRNA levels 13-fold due to post-transcriptional gene regulation. When two animal models of hepatic fibrosis, murine schistosomiasis and CCl4-treated rats, were examined, they both exhibited increased levels of TGF-β1 gene expression at times that somewhat preceded the increase in collagen synthesis. In contrast, in murine schistosomiasis, mRNA levels of tumor necrosis factor and interleukin-1 peaked early in the fibrogenic process. Immunohistochemical analysis showed TGF-β1 to be present in normal mouse liver and to be markedly increased in mice infected with schistosomiasis. TGF-β1 appeared in the hepatic parenchyma, primarily in hepatocytes. These findings strongly suggest a role for TGF-β1 in a pathophysiological state.

Original languageEnglish (US)
Pages (from-to)2477-2482
Number of pages6
JournalJournal of Cell Biology
Volume108
Issue number6
StatePublished - 1989

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Transforming Growth Factors
Fibrosis
Liver
Schistosomiasis
Hepatocytes
Collagen
Messenger RNA
Collagen Type I
Interleukin-1
In Vitro Techniques
Cultured Cells
Animal Models
Tumor Necrosis Factor-alpha
Gene Expression
Genes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Czaja, M. J., Weiner, F. R., Flanders, K. C., Giambrone, M. A., Wind, R., Biempica, L., & Zern, M. A. (1989). In vitro and in vivo association of transforming growth factor-β1 with hepatic fibrosis. Journal of Cell Biology, 108(6), 2477-2482.

In vitro and in vivo association of transforming growth factor-β1 with hepatic fibrosis. / Czaja, M. J.; Weiner, F. R.; Flanders, K. C.; Giambrone, M. A.; Wind, R.; Biempica, L.; Zern, M. A.

In: Journal of Cell Biology, Vol. 108, No. 6, 1989, p. 2477-2482.

Research output: Contribution to journalArticle

Czaja, MJ, Weiner, FR, Flanders, KC, Giambrone, MA, Wind, R, Biempica, L & Zern, MA 1989, 'In vitro and in vivo association of transforming growth factor-β1 with hepatic fibrosis', Journal of Cell Biology, vol. 108, no. 6, pp. 2477-2482.
Czaja MJ, Weiner FR, Flanders KC, Giambrone MA, Wind R, Biempica L et al. In vitro and in vivo association of transforming growth factor-β1 with hepatic fibrosis. Journal of Cell Biology. 1989;108(6):2477-2482.
Czaja, M. J. ; Weiner, F. R. ; Flanders, K. C. ; Giambrone, M. A. ; Wind, R. ; Biempica, L. ; Zern, M. A. / In vitro and in vivo association of transforming growth factor-β1 with hepatic fibrosis. In: Journal of Cell Biology. 1989 ; Vol. 108, No. 6. pp. 2477-2482.
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