In vitro and in vivo association of transforming growth factor-β1 with hepatic fibrosis

M. J. Czaja, F. R. Weiner, K. C. Flanders, M. A. Giambrone, R. Wind, L. Biempica, M. A. Zern

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363 Scopus citations

Abstract

Despite extensive efforts, little progress has been made in identifying the factors that induce hepatic fibrosis. Transforming growth factor-β (TGF-β) has been shown to enhance collagen production, therefore its role in hepatic fibrosis was investigated. Treatment of cultured hepatic cells with TGF-β1 increased type I procollagen mRNA levels 13-fold due to post-transcriptional gene regulation. When two animal models of hepatic fibrosis, murine schistosomiasis and CCl4-treated rats, were examined, they both exhibited increased levels of TGF-β1 gene expression at times that somewhat preceded the increase in collagen synthesis. In contrast, in murine schistosomiasis, mRNA levels of tumor necrosis factor and interleukin-1 peaked early in the fibrogenic process. Immunohistochemical analysis showed TGF-β1 to be present in normal mouse liver and to be markedly increased in mice infected with schistosomiasis. TGF-β1 appeared in the hepatic parenchyma, primarily in hepatocytes. These findings strongly suggest a role for TGF-β1 in a pathophysiological state.

Original languageEnglish (US)
Pages (from-to)2477-2482
Number of pages6
JournalJournal of Cell Biology
Volume108
Issue number6
DOIs
StatePublished - Jan 1 1989

ASJC Scopus subject areas

  • Cell Biology

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    Czaja, M. J., Weiner, F. R., Flanders, K. C., Giambrone, M. A., Wind, R., Biempica, L., & Zern, M. A. (1989). In vitro and in vivo association of transforming growth factor-β1 with hepatic fibrosis. Journal of Cell Biology, 108(6), 2477-2482. https://doi.org/10.1083/jcb.108.6.2477