Improvement of xylose utilization in Clostridium acetobutylicum via expression of the talA gene encoding transaldolase from Escherichia coli

Yang Gu, Jian Li, Lei Zhang, Jun Chen, Lixia Niu, Yunliu Yang, Sheng Yang, Weihong Jiang

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

Clostridium acetobutylicum ATCC 824 was metabolically engineered for improved xylose utilization. The gene talA, which encodes transaldolase from Escherichia coli K-12, was cloned and overexpressed in C. acetobutylicum ATCC 824. Compared with C. acetobutylicum ATCC 824 (824-WT), the transformant bearing the E. coli talA gene (824-TAL) showed improved ability on xylose utilization and solvents production using xylose as the sole carbon source. During the fermentation of xylose and glucose mixtures with three xylose/glucose ratios (approximately 1:2, 1:1 and 2:1), the rate of xylose consumption and final solvents titers of 824-TAL were all higher than those of 824-WT, despite glucose repression on xylose uptake still existing. These results suggest that the insufficiency of transaldolase in the pentose phosphate pathway (PPP) of C. acetobutylicum is one of the bottlenecks for xylose metabolism and therefore, overexpressing the gene encoding transaldolase is able to improve xylose utilization and solvent production.

Original languageEnglish (US)
Pages (from-to)284-287
Number of pages4
JournalJournal of Biotechnology
Volume143
Issue number4
DOIs
StatePublished - Sep 25 2009
Externally publishedYes

Keywords

  • Clostridium acetobutylicum
  • Overexpressing transaldolase
  • Solvent production
  • Xylose utilization

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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