TY - JOUR
T1 - Improvement of xylose utilization in Clostridium acetobutylicum via expression of the talA gene encoding transaldolase from Escherichia coli
AU - Gu, Yang
AU - Li, Jian
AU - Zhang, Lei
AU - Chen, Jun
AU - Niu, Lixia
AU - Yang, Yunliu
AU - Yang, Sheng
AU - Jiang, Weihong
N1 - Funding Information:
We are grateful to Prof. P. Dürre (University Ulm, Germany) for the generous gift of the plasmid pIMP1 and the E. coli ER2275 (pAN1) strain. This work was supported by the National Basic Research Program of China (973: 2007CB707803), the National High-tech Research and Development Program of China (863: 2006AA02Z237; 863: 2007AA05Z407), the Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-YW-G-007) and the Planned Scientific Program of Science and Technology Commission of Shanghai Municipality (08dz1207100).
PY - 2009/9/25
Y1 - 2009/9/25
N2 - Clostridium acetobutylicum ATCC 824 was metabolically engineered for improved xylose utilization. The gene talA, which encodes transaldolase from Escherichia coli K-12, was cloned and overexpressed in C. acetobutylicum ATCC 824. Compared with C. acetobutylicum ATCC 824 (824-WT), the transformant bearing the E. coli talA gene (824-TAL) showed improved ability on xylose utilization and solvents production using xylose as the sole carbon source. During the fermentation of xylose and glucose mixtures with three xylose/glucose ratios (approximately 1:2, 1:1 and 2:1), the rate of xylose consumption and final solvents titers of 824-TAL were all higher than those of 824-WT, despite glucose repression on xylose uptake still existing. These results suggest that the insufficiency of transaldolase in the pentose phosphate pathway (PPP) of C. acetobutylicum is one of the bottlenecks for xylose metabolism and therefore, overexpressing the gene encoding transaldolase is able to improve xylose utilization and solvent production.
AB - Clostridium acetobutylicum ATCC 824 was metabolically engineered for improved xylose utilization. The gene talA, which encodes transaldolase from Escherichia coli K-12, was cloned and overexpressed in C. acetobutylicum ATCC 824. Compared with C. acetobutylicum ATCC 824 (824-WT), the transformant bearing the E. coli talA gene (824-TAL) showed improved ability on xylose utilization and solvents production using xylose as the sole carbon source. During the fermentation of xylose and glucose mixtures with three xylose/glucose ratios (approximately 1:2, 1:1 and 2:1), the rate of xylose consumption and final solvents titers of 824-TAL were all higher than those of 824-WT, despite glucose repression on xylose uptake still existing. These results suggest that the insufficiency of transaldolase in the pentose phosphate pathway (PPP) of C. acetobutylicum is one of the bottlenecks for xylose metabolism and therefore, overexpressing the gene encoding transaldolase is able to improve xylose utilization and solvent production.
KW - Clostridium acetobutylicum
KW - Overexpressing transaldolase
KW - Solvent production
KW - Xylose utilization
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U2 - 10.1016/j.jbiotec.2009.08.009
DO - 10.1016/j.jbiotec.2009.08.009
M3 - Article
C2 - 19695296
AN - SCOPUS:70249106057
SN - 0168-1656
VL - 143
SP - 284
EP - 287
JO - Journal of Biotechnology
JF - Journal of Biotechnology
IS - 4
ER -