Improved transfection and new selectable markers for the rodent malaria parasite Plasmodium yoelii

Artemio M. Jongco, Li Min Ting, Vandana Thathy, Maria M. Mota, Kami Kim

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The rodent malaria Plasmodium yoelii is a useful model to study protective immunity to pre-erythrocytic stages of infection, pathogenesis of erythrocytic stages, and vaccine development. However, the utility of the P. yoelii model system has not been fully realized because transfection and genetic manipulation methodologies for this rodent species are less developed than that of another rodent species Plasmodium berghei. Here we report improved transfection efficiency using the AMAXA nucleofector® system compared to conventional transfection methodologies. We also show that heterologous promoters from P. berghei can be used to drive expression of a green fluorescent protein (GFP) reporter protein in P. yoelii. In an effort to develop additional selectable markers for this parasite, we also tested positive selectable markers that have been used successfully in P. falciparum and P. berghei. Human dihydrofolate reductase (hdhfr) and Toxoplasma gondii dihydrofolate reductase-thymidylate synthase (Tgdhfr-ts) conferred drug resistance to WR99210 and pyrimethamine, respectively, when introduced as episomes. These improvements should make genetic manipulation of P. yoelii more amenable and facilitate further studies of host-parasite interactions using this attractive rodent model.

Original languageEnglish (US)
Pages (from-to)242-250
Number of pages9
JournalMolecular and Biochemical Parasitology
Volume146
Issue number2
DOIs
StatePublished - Apr 2006

Fingerprint

Plasmodium yoelii
Plasmodium berghei
Malaria
Transfection
Rodentia
Parasites
Host-Parasite Interactions
Pyrimethamine
Tetrahydrofolate Dehydrogenase
Toxoplasma
Green Fluorescent Proteins
Drug Resistance
Immunity
Plasmids
Vaccines
Infection
Proteins

Keywords

  • FACS
  • GFP
  • Nucleofector®
  • Plasmodium yoelii
  • Transfection

ASJC Scopus subject areas

  • Molecular Biology
  • Parasitology

Cite this

Improved transfection and new selectable markers for the rodent malaria parasite Plasmodium yoelii. / Jongco, Artemio M.; Ting, Li Min; Thathy, Vandana; Mota, Maria M.; Kim, Kami.

In: Molecular and Biochemical Parasitology, Vol. 146, No. 2, 04.2006, p. 242-250.

Research output: Contribution to journalArticle

Jongco, Artemio M. ; Ting, Li Min ; Thathy, Vandana ; Mota, Maria M. ; Kim, Kami. / Improved transfection and new selectable markers for the rodent malaria parasite Plasmodium yoelii. In: Molecular and Biochemical Parasitology. 2006 ; Vol. 146, No. 2. pp. 242-250.
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