Impaired phagocytosis in caveolin-1 deficient macrophages

Jiangwei Li, Alexis Scherl, Freddy Medina, Philippe G. Frank, Richard N. Kitsis, Herbert B. Tanowitz, Federico Sotgia, Michael P. Lisanti

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Caveolae are plasma membrane invaginations that function as important regulators of numerous cellular processes, including signal transduction, cholesterol trafficking, and endocytosis. Caveolin-1 (Cav-1) constitutes the main structural protein of caveolae membranes. Here, we report an in vivo increase in the number of apoptotic cells in the thymus and spleen of Cav-1 deficient mice, following whole-body γ-irradiation. We demonstrate that this increase in apoptotic cells is not due to increased apoptosis in lymphocytes per se, which normally do not express Cav-1, but rather to the decreased phagocytic clearance of apoptotic cells by macrophages, which do express Cav-1. Utilizing in vitro phagocytosis assays of both apoptotic thymocytes and Escherichia coli K-12 BioParticles, we demonstrate that the loss of Cav-1 decreases the phagocytic ability of thioglycollate-elicited peritoneal macrophages. We suggest that impaired macrophage phagocytosis in Cav-1 knockout mice could have implications for altered innate immunity against pathogens, the regulation of inflammatory responses, and the development of autoimmune disease.

Original languageEnglish (US)
Pages (from-to)1597-1605
Number of pages9
JournalCell Cycle
Volume4
Issue number11
StatePublished - Nov 2005

Fingerprint

Caveolin 1
Macrophages
Phagocytosis
Caveolae
Cells
Thioglycolates
Thymus
Signal transduction
Lymphocytes
Whole-Body Irradiation
Peritoneal Macrophages
Pathogens
Thymocytes
Cell membranes
Endocytosis
Innate Immunity
Knockout Mice
Thymus Gland
Escherichia coli
Autoimmune Diseases

Keywords

  • Apoptosis
  • Autoimmune disease
  • Caveolae
  • Caveolin-1
  • Innate immunity
  • Macrophage
  • Phagocytosis

ASJC Scopus subject areas

  • Cell Biology
  • Biochemistry
  • Molecular Biology

Cite this

Li, J., Scherl, A., Medina, F., Frank, P. G., Kitsis, R. N., Tanowitz, H. B., ... Lisanti, M. P. (2005). Impaired phagocytosis in caveolin-1 deficient macrophages. Cell Cycle, 4(11), 1597-1605.

Impaired phagocytosis in caveolin-1 deficient macrophages. / Li, Jiangwei; Scherl, Alexis; Medina, Freddy; Frank, Philippe G.; Kitsis, Richard N.; Tanowitz, Herbert B.; Sotgia, Federico; Lisanti, Michael P.

In: Cell Cycle, Vol. 4, No. 11, 11.2005, p. 1597-1605.

Research output: Contribution to journalArticle

Li, J, Scherl, A, Medina, F, Frank, PG, Kitsis, RN, Tanowitz, HB, Sotgia, F & Lisanti, MP 2005, 'Impaired phagocytosis in caveolin-1 deficient macrophages', Cell Cycle, vol. 4, no. 11, pp. 1597-1605.
Li J, Scherl A, Medina F, Frank PG, Kitsis RN, Tanowitz HB et al. Impaired phagocytosis in caveolin-1 deficient macrophages. Cell Cycle. 2005 Nov;4(11):1597-1605.
Li, Jiangwei ; Scherl, Alexis ; Medina, Freddy ; Frank, Philippe G. ; Kitsis, Richard N. ; Tanowitz, Herbert B. ; Sotgia, Federico ; Lisanti, Michael P. / Impaired phagocytosis in caveolin-1 deficient macrophages. In: Cell Cycle. 2005 ; Vol. 4, No. 11. pp. 1597-1605.
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