Impact of reproductive aging on the vaginal microbiome and soluble immune mediators in women living with and at-risk for HIV infection

Kerry J. Murphy, Marla J. Keller, Kathryn Anastos, Shada Sinclair, J. Cooper Devlin, Qiuhu Shi, Donald R. Hoover, Brian Starkman, Jamie McGillick, Caroline Mullis, Howard Minkoff, Maria Gloria Dominguez-Bello, Betsy Herold

Research output: Contribution to journalArticle

Abstract

Background Reproductive aging may impact the vaginal microbiome and genital tract mucosal immune environment and contribute to genital tract health in women living with and at-risk for HIV infection. Methods A cross-sectional study of 102 HIV+ (51 premenopausal, 51 postmenopausal) and 39 HIV-uninfected (HIV-) (20 premenopausal, 19 postmenopausal) women was performed in Bronx and Brooklyn, NY. Cervicovaginal lavage (CVL) was collected for quantification of innate antimicrobial activity against E. coli, HSV-2 and HIV and immune mediators by Luminex and ELISA. Microbiome studies by qPCR and 16S rRNA sequencing were performed on vaginal swabs. Results HIV+ postmenopausal compared to premenopausal participants had lower median E. coli bactericidal activity (41% vs. 62%, p = 0.001), lower median gene copies of Lactobacillus crispatus (p = 0.005) and Lactobacillus iners (p = 0.019), lower proportions of Lactobacillus iners, higher proportions of Gardnerella and Atopobium vaginae and lower levels of human beta defensins (HBD-2, HBD-3) and secretory leukocyte protease inhibitor (SLPI), p<0.001. HSV-2 inhibitory activity was higher in HIV+ postmenopausal compared to premenopausal participants (37% vs. 17%, p = 0.001) and correlated with the proinflammatory molecules interleukin (IL) 6, IL-8, human neutrophil peptide (HNP) 1–3, lactoferrin and fibronectin. Similar trends were observed in HIV- postmenopausal compared to premenopausal participants. HIV inhibitory activity did not differ by reproductive status in the HIV+ participants but was significantly higher in HIV- postmenopausal compared to premenopausal participants and in participants with suppressed plasma viral load, and inversely correlated with gene copies of G. vaginalis and BVAB2. A significant proportion of HIV+ participants on ART exhibited HIV enhancing activity.

Original languageEnglish (US)
Article numbere0216049
JournalPLoS ONE
Volume14
Issue number4
DOIs
StatePublished - Apr 1 2019

Fingerprint

Microbiota
HIV infections
Atopobium vaginae
Lactobacillus
Escherichia coli
genitalia
HIV Infections
Gardnerella
Lactobacillus crispatus
Genes
Secretory Leukocyte Peptidase Inhibitor
Aging of materials
HIV
beta-Defensins
women's health
Lactoferrin
lactoferrin
fibronectins
interleukin-8
viral load

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Impact of reproductive aging on the vaginal microbiome and soluble immune mediators in women living with and at-risk for HIV infection. / Murphy, Kerry J.; Keller, Marla J.; Anastos, Kathryn; Sinclair, Shada; Cooper Devlin, J.; Shi, Qiuhu; Hoover, Donald R.; Starkman, Brian; McGillick, Jamie; Mullis, Caroline; Minkoff, Howard; Dominguez-Bello, Maria Gloria; Herold, Betsy.

In: PLoS ONE, Vol. 14, No. 4, e0216049, 01.04.2019.

Research output: Contribution to journalArticle

Murphy, KJ, Keller, MJ, Anastos, K, Sinclair, S, Cooper Devlin, J, Shi, Q, Hoover, DR, Starkman, B, McGillick, J, Mullis, C, Minkoff, H, Dominguez-Bello, MG & Herold, B 2019, 'Impact of reproductive aging on the vaginal microbiome and soluble immune mediators in women living with and at-risk for HIV infection', PLoS ONE, vol. 14, no. 4, e0216049. https://doi.org/10.1371/journal.pone.0216049
Murphy, Kerry J. ; Keller, Marla J. ; Anastos, Kathryn ; Sinclair, Shada ; Cooper Devlin, J. ; Shi, Qiuhu ; Hoover, Donald R. ; Starkman, Brian ; McGillick, Jamie ; Mullis, Caroline ; Minkoff, Howard ; Dominguez-Bello, Maria Gloria ; Herold, Betsy. / Impact of reproductive aging on the vaginal microbiome and soluble immune mediators in women living with and at-risk for HIV infection. In: PLoS ONE. 2019 ; Vol. 14, No. 4.
@article{46fbccf6755b4dc1859c2b31d273a900,
title = "Impact of reproductive aging on the vaginal microbiome and soluble immune mediators in women living with and at-risk for HIV infection",
abstract = "Background Reproductive aging may impact the vaginal microbiome and genital tract mucosal immune environment and contribute to genital tract health in women living with and at-risk for HIV infection. Methods A cross-sectional study of 102 HIV+ (51 premenopausal, 51 postmenopausal) and 39 HIV-uninfected (HIV-) (20 premenopausal, 19 postmenopausal) women was performed in Bronx and Brooklyn, NY. Cervicovaginal lavage (CVL) was collected for quantification of innate antimicrobial activity against E. coli, HSV-2 and HIV and immune mediators by Luminex and ELISA. Microbiome studies by qPCR and 16S rRNA sequencing were performed on vaginal swabs. Results HIV+ postmenopausal compared to premenopausal participants had lower median E. coli bactericidal activity (41{\%} vs. 62{\%}, p = 0.001), lower median gene copies of Lactobacillus crispatus (p = 0.005) and Lactobacillus iners (p = 0.019), lower proportions of Lactobacillus iners, higher proportions of Gardnerella and Atopobium vaginae and lower levels of human beta defensins (HBD-2, HBD-3) and secretory leukocyte protease inhibitor (SLPI), p<0.001. HSV-2 inhibitory activity was higher in HIV+ postmenopausal compared to premenopausal participants (37{\%} vs. 17{\%}, p = 0.001) and correlated with the proinflammatory molecules interleukin (IL) 6, IL-8, human neutrophil peptide (HNP) 1–3, lactoferrin and fibronectin. Similar trends were observed in HIV- postmenopausal compared to premenopausal participants. HIV inhibitory activity did not differ by reproductive status in the HIV+ participants but was significantly higher in HIV- postmenopausal compared to premenopausal participants and in participants with suppressed plasma viral load, and inversely correlated with gene copies of G. vaginalis and BVAB2. A significant proportion of HIV+ participants on ART exhibited HIV enhancing activity.",
author = "Murphy, {Kerry J.} and Keller, {Marla J.} and Kathryn Anastos and Shada Sinclair and {Cooper Devlin}, J. and Qiuhu Shi and Hoover, {Donald R.} and Brian Starkman and Jamie McGillick and Caroline Mullis and Howard Minkoff and Dominguez-Bello, {Maria Gloria} and Betsy Herold",
year = "2019",
month = "4",
day = "1",
doi = "10.1371/journal.pone.0216049",
language = "English (US)",
volume = "14",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "4",

}

TY - JOUR

T1 - Impact of reproductive aging on the vaginal microbiome and soluble immune mediators in women living with and at-risk for HIV infection

AU - Murphy, Kerry J.

AU - Keller, Marla J.

AU - Anastos, Kathryn

AU - Sinclair, Shada

AU - Cooper Devlin, J.

AU - Shi, Qiuhu

AU - Hoover, Donald R.

AU - Starkman, Brian

AU - McGillick, Jamie

AU - Mullis, Caroline

AU - Minkoff, Howard

AU - Dominguez-Bello, Maria Gloria

AU - Herold, Betsy

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Background Reproductive aging may impact the vaginal microbiome and genital tract mucosal immune environment and contribute to genital tract health in women living with and at-risk for HIV infection. Methods A cross-sectional study of 102 HIV+ (51 premenopausal, 51 postmenopausal) and 39 HIV-uninfected (HIV-) (20 premenopausal, 19 postmenopausal) women was performed in Bronx and Brooklyn, NY. Cervicovaginal lavage (CVL) was collected for quantification of innate antimicrobial activity against E. coli, HSV-2 and HIV and immune mediators by Luminex and ELISA. Microbiome studies by qPCR and 16S rRNA sequencing were performed on vaginal swabs. Results HIV+ postmenopausal compared to premenopausal participants had lower median E. coli bactericidal activity (41% vs. 62%, p = 0.001), lower median gene copies of Lactobacillus crispatus (p = 0.005) and Lactobacillus iners (p = 0.019), lower proportions of Lactobacillus iners, higher proportions of Gardnerella and Atopobium vaginae and lower levels of human beta defensins (HBD-2, HBD-3) and secretory leukocyte protease inhibitor (SLPI), p<0.001. HSV-2 inhibitory activity was higher in HIV+ postmenopausal compared to premenopausal participants (37% vs. 17%, p = 0.001) and correlated with the proinflammatory molecules interleukin (IL) 6, IL-8, human neutrophil peptide (HNP) 1–3, lactoferrin and fibronectin. Similar trends were observed in HIV- postmenopausal compared to premenopausal participants. HIV inhibitory activity did not differ by reproductive status in the HIV+ participants but was significantly higher in HIV- postmenopausal compared to premenopausal participants and in participants with suppressed plasma viral load, and inversely correlated with gene copies of G. vaginalis and BVAB2. A significant proportion of HIV+ participants on ART exhibited HIV enhancing activity.

AB - Background Reproductive aging may impact the vaginal microbiome and genital tract mucosal immune environment and contribute to genital tract health in women living with and at-risk for HIV infection. Methods A cross-sectional study of 102 HIV+ (51 premenopausal, 51 postmenopausal) and 39 HIV-uninfected (HIV-) (20 premenopausal, 19 postmenopausal) women was performed in Bronx and Brooklyn, NY. Cervicovaginal lavage (CVL) was collected for quantification of innate antimicrobial activity against E. coli, HSV-2 and HIV and immune mediators by Luminex and ELISA. Microbiome studies by qPCR and 16S rRNA sequencing were performed on vaginal swabs. Results HIV+ postmenopausal compared to premenopausal participants had lower median E. coli bactericidal activity (41% vs. 62%, p = 0.001), lower median gene copies of Lactobacillus crispatus (p = 0.005) and Lactobacillus iners (p = 0.019), lower proportions of Lactobacillus iners, higher proportions of Gardnerella and Atopobium vaginae and lower levels of human beta defensins (HBD-2, HBD-3) and secretory leukocyte protease inhibitor (SLPI), p<0.001. HSV-2 inhibitory activity was higher in HIV+ postmenopausal compared to premenopausal participants (37% vs. 17%, p = 0.001) and correlated with the proinflammatory molecules interleukin (IL) 6, IL-8, human neutrophil peptide (HNP) 1–3, lactoferrin and fibronectin. Similar trends were observed in HIV- postmenopausal compared to premenopausal participants. HIV inhibitory activity did not differ by reproductive status in the HIV+ participants but was significantly higher in HIV- postmenopausal compared to premenopausal participants and in participants with suppressed plasma viral load, and inversely correlated with gene copies of G. vaginalis and BVAB2. A significant proportion of HIV+ participants on ART exhibited HIV enhancing activity.

UR - http://www.scopus.com/inward/record.url?scp=85064747384&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85064747384&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0216049

DO - 10.1371/journal.pone.0216049

M3 - Article

C2 - 31026271

AN - SCOPUS:85064747384

VL - 14

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 4

M1 - e0216049

ER -