Immunosuppressive property of dried human amniotic membrane

Choul Yong Park, Sahar Kohanim, Lei Zhu, Peter L. Gehlbach, Roy S. Chuck

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Purpose: To report the immunosuppressive property of dry human amniotic membrane (dHAM). Methods: Mouse splenocytes harvested from Balb/c mice were stimulated using functional-grade anti-CD3e antibodies for 4 days either with or without either of 2 commercial types of dHAM (Ambiodry 1 & 2, IOP Inc., Costa Mesa, Calif., USA) added to the culture media. The cell proliferation assay was performed to analyze the extent of splenocyte proliferation. Results: dHAMs significantly suppressed mouse splenocyte proliferation compared to control. The suppression by dHAM with intact amniotic epithelium (Ambiodry 2) was significantly stronger than dHAM without epithelium (Ambiodry 1). Conclusion: The immunosuppressive property of dHAM was demonstrated using an alloreactive splenocyte proliferation assay.

Original languageEnglish (US)
Pages (from-to)112-113
Number of pages2
JournalOphthalmic Research
Volume41
Issue number2
DOIs
StatePublished - Jan 2009
Externally publishedYes

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Amnion
Immunosuppressive Agents
Epithelium
Culture Media
Anti-Idiotypic Antibodies
Cell Proliferation

Keywords

  • Amniotic membrane
  • Cryopreservation
  • Immune suppression
  • Ocular surface
  • T cell proliferation

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Immunosuppressive property of dried human amniotic membrane. / Park, Choul Yong; Kohanim, Sahar; Zhu, Lei; Gehlbach, Peter L.; Chuck, Roy S.

In: Ophthalmic Research, Vol. 41, No. 2, 01.2009, p. 112-113.

Research output: Contribution to journalArticle

Park, CY, Kohanim, S, Zhu, L, Gehlbach, PL & Chuck, RS 2009, 'Immunosuppressive property of dried human amniotic membrane', Ophthalmic Research, vol. 41, no. 2, pp. 112-113. https://doi.org/10.1159/000187629
Park, Choul Yong ; Kohanim, Sahar ; Zhu, Lei ; Gehlbach, Peter L. ; Chuck, Roy S. / Immunosuppressive property of dried human amniotic membrane. In: Ophthalmic Research. 2009 ; Vol. 41, No. 2. pp. 112-113.
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