Immunosuppressive property of dried human amniotic membrane

Choul Yong Park, Sahar Kohanim, Lei Zhu, Peter L. Gehlbach, Roy S. Chuck

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Purpose: To report the immunosuppressive property of dry human amniotic membrane (dHAM). Methods: Mouse splenocytes harvested from Balb/c mice were stimulated using functional-grade anti-CD3e antibodies for 4 days either with or without either of 2 commercial types of dHAM (Ambiodry 1 & 2, IOP Inc., Costa Mesa, Calif., USA) added to the culture media. The cell proliferation assay was performed to analyze the extent of splenocyte proliferation. Results: dHAMs significantly suppressed mouse splenocyte proliferation compared to control. The suppression by dHAM with intact amniotic epithelium (Ambiodry 2) was significantly stronger than dHAM without epithelium (Ambiodry 1). Conclusion: The immunosuppressive property of dHAM was demonstrated using an alloreactive splenocyte proliferation assay.

Original languageEnglish (US)
Pages (from-to)112-113
Number of pages2
JournalOphthalmic Research
Volume41
Issue number2
DOIs
StatePublished - Jan 1 2009
Externally publishedYes

Keywords

  • Amniotic membrane
  • Cryopreservation
  • Immune suppression
  • Ocular surface
  • T cell proliferation

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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