Immunoreactivity to antinucleoside antibodies in camptothecin treated HeLa cells

D. Liebeskind, Susan Band Horwitz, M. S. Horwitz, K. C. Hsu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

HeLa cells, incubated with camptothecin during the G 1 phase of the cell cycle, show nuclear fluorescence with fluorescein-labeled antinucleoside antibodies. If the G 1 cells are washed free of the drug, the cells no longer demonstrate nuclear fluorescence. Since these antibodies react only with single-stranded DNA, the positive staining in camptothecin-treated G 1 cells suggests that the drug induces denatured regions in DNA. Fluorescent antinucleoside antibodies may be a useful technique for the observation of drug-induced changes in DNA during the G1 phase of the cell cycle.

Original languageEnglish (US)
Pages (from-to)174-178
Number of pages5
JournalExperimental Cell Research
Volume86
Issue number1
DOIs
StatePublished - 1974

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Camptothecin
HeLa Cells
Gastrin-Secreting Cells
Antibodies
Cell Cycle
Fluorescence
Pharmaceutical Preparations
Single-Stranded DNA
DNA
G1 Phase
Fluorescein
Observation
Staining and Labeling

ASJC Scopus subject areas

  • Cell Biology

Cite this

Immunoreactivity to antinucleoside antibodies in camptothecin treated HeLa cells. / Liebeskind, D.; Band Horwitz, Susan; Horwitz, M. S.; Hsu, K. C.

In: Experimental Cell Research, Vol. 86, No. 1, 1974, p. 174-178.

Research output: Contribution to journalArticle

Liebeskind, D. ; Band Horwitz, Susan ; Horwitz, M. S. ; Hsu, K. C. / Immunoreactivity to antinucleoside antibodies in camptothecin treated HeLa cells. In: Experimental Cell Research. 1974 ; Vol. 86, No. 1. pp. 174-178.
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