Immunoreactivity to antinucleoside antibodies in camptothecin treated HeLa cells

D. Liebeskind, S. B. Horwitz, M. S. Horwitz, K. C. Hsu

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

HeLa cells, incubated with camptothecin during the G 1 phase of the cell cycle, show nuclear fluorescence with fluorescein-labeled antinucleoside antibodies. If the G 1 cells are washed free of the drug, the cells no longer demonstrate nuclear fluorescence. Since these antibodies react only with single-stranded DNA, the positive staining in camptothecin-treated G 1 cells suggests that the drug induces denatured regions in DNA. Fluorescent antinucleoside antibodies may be a useful technique for the observation of drug-induced changes in DNA during the G1 phase of the cell cycle.

Original languageEnglish (US)
Pages (from-to)174-178
Number of pages5
JournalExperimental Cell Research
Volume86
Issue number1
DOIs
StatePublished - May 1974

ASJC Scopus subject areas

  • Cell Biology

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