TY - JOUR
T1 - Immunoprophylaxis of multi-antigen peptide (MAP) vaccine for human lymphatic filariasis
AU - Immanuel, Christiana
AU - Ramanathan, Aparnaa
AU - Balasubramaniyan, Malathi
AU - Khatri, Vishal Kishor
AU - Amdare, Nitin Purushottam
AU - Rao, Donthamsetty Nageswara
AU - Reddy, Maryada Venkata Rami
AU - Perumal, Kaliraj
N1 - Publisher Copyright:
© 2017, Springer Science+Business Media New York.
PY - 2017/6/1
Y1 - 2017/6/1
N2 - Human lymphatic filariasis, the parasitic disease caused by the filarial nematodes Wuchereria bancrofti, Brugia malayi, and Brugia timori, is ranked as the second most complex clinical condition leading to permanent and long-term disability. The multiple antigen peptide (MAP) approach is an effective method to chemically synthesize and deliver multiple T and B cell epitopes as the constituents of a single immunogen. Here, we report on the design, chemical synthesis, and immunoprophylaxis of three epitopes that have been identified from promising vaccine candidates reported in our previous studies, constructed as MAP on an inert lysine core for human lymphatic filariasis in Jird model. Two epitopes from Thioredoxin and one epitope from Transglutaminase were constructed as MAP in an inert lysine core. The immunoprophylaxis of the synthetic vaccine construct studied in Jird models showed protective antibody (1 in 64,000 titer) and cellular immune response. Thioredoxin-Transglutaminase MAP (TT MAP) conferred a significantly high protection of 63.04% compared to control (8.5%). Multi-antigen peptide vaccine is one best approach to provide immunity against multiple antigens delivered by the complex filarial parasite.
AB - Human lymphatic filariasis, the parasitic disease caused by the filarial nematodes Wuchereria bancrofti, Brugia malayi, and Brugia timori, is ranked as the second most complex clinical condition leading to permanent and long-term disability. The multiple antigen peptide (MAP) approach is an effective method to chemically synthesize and deliver multiple T and B cell epitopes as the constituents of a single immunogen. Here, we report on the design, chemical synthesis, and immunoprophylaxis of three epitopes that have been identified from promising vaccine candidates reported in our previous studies, constructed as MAP on an inert lysine core for human lymphatic filariasis in Jird model. Two epitopes from Thioredoxin and one epitope from Transglutaminase were constructed as MAP in an inert lysine core. The immunoprophylaxis of the synthetic vaccine construct studied in Jird models showed protective antibody (1 in 64,000 titer) and cellular immune response. Thioredoxin-Transglutaminase MAP (TT MAP) conferred a significantly high protection of 63.04% compared to control (8.5%). Multi-antigen peptide vaccine is one best approach to provide immunity against multiple antigens delivered by the complex filarial parasite.
KW - Filariasis
KW - Multi-antigen peptide (MAP)
KW - Synthetic vaccine
KW - Thioredoxin
KW - Transglutaminase
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UR - http://www.scopus.com/inward/citedby.url?scp=85018806127&partnerID=8YFLogxK
U2 - 10.1007/s12026-017-8911-5
DO - 10.1007/s12026-017-8911-5
M3 - Article
C2 - 28432603
AN - SCOPUS:85018806127
SN - 0257-277X
VL - 65
SP - 729
EP - 738
JO - Immunologic Research
JF - Immunologic Research
IS - 3
ER -