Immunoprophylaxis of multi-antigen peptide (MAP) vaccine for human lymphatic filariasis

Christiana Immanuel, Aparnaa Ramanathan, Malathi Balasubramaniyan, Vishal Kishor Khatri, Nitin Purushottam Amdare, Donthamsetty Nageswara Rao, Maryada Venkata Rami Reddy, Kaliraj Perumal

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Human lymphatic filariasis, the parasitic disease caused by the filarial nematodes Wuchereria bancrofti, Brugia malayi, and Brugia timori, is ranked as the second most complex clinical condition leading to permanent and long-term disability. The multiple antigen peptide (MAP) approach is an effective method to chemically synthesize and deliver multiple T and B cell epitopes as the constituents of a single immunogen. Here, we report on the design, chemical synthesis, and immunoprophylaxis of three epitopes that have been identified from promising vaccine candidates reported in our previous studies, constructed as MAP on an inert lysine core for human lymphatic filariasis in Jird model. Two epitopes from Thioredoxin and one epitope from Transglutaminase were constructed as MAP in an inert lysine core. The immunoprophylaxis of the synthetic vaccine construct studied in Jird models showed protective antibody (1 in 64,000 titer) and cellular immune response. Thioredoxin-Transglutaminase MAP (TT MAP) conferred a significantly high protection of 63.04% compared to control (8.5%). Multi-antigen peptide vaccine is one best approach to provide immunity against multiple antigens delivered by the complex filarial parasite.

Original languageEnglish (US)
Pages (from-to)729-738
Number of pages10
JournalImmunologic Research
Volume65
Issue number3
DOIs
StatePublished - Jun 1 2017
Externally publishedYes

Keywords

  • Filariasis
  • Multi-antigen peptide (MAP)
  • Synthetic vaccine
  • Thioredoxin
  • Transglutaminase

ASJC Scopus subject areas

  • Immunology

Fingerprint

Dive into the research topics of 'Immunoprophylaxis of multi-antigen peptide (MAP) vaccine for human lymphatic filariasis'. Together they form a unique fingerprint.

Cite this