Immunoglobulin heavy chain class switch from IgM to IgG in a hybridoma

P. Thammana, Matthew D. Scharff

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A subclone of an IgM-producing hybridoma has been identified which has switched to producing an IgG1 antibody. The parent hybridoma, PC-140, produces an antibody which binds phosphorylcholine and reacts with monoclonal antibodies that recognize myelomas of the T-15 idiotype. The IgG1 antibody binds phosphorylcholine with the same affinity as the parental IgM and also reacts with the anti-T-15 monoclonal antibodies. While the IgM-producing parent hybridoma does not express detectable surface IgM, the IgG1-producing subclone produces both membrane and secreted IgG1.

Original languageEnglish (US)
Pages (from-to)614-619
Number of pages6
JournalEuropean Journal of Immunology
Volume13
Issue number8
StatePublished - 1983

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Immunoglobulin Heavy Chains
Hybridomas
Immunoglobulin M
Immunoglobulin G
Phosphorylcholine
Antibodies
Monoclonal Antibodies
Membranes

ASJC Scopus subject areas

  • Immunology

Cite this

Immunoglobulin heavy chain class switch from IgM to IgG in a hybridoma. / Thammana, P.; Scharff, Matthew D.

In: European Journal of Immunology, Vol. 13, No. 8, 1983, p. 614-619.

Research output: Contribution to journalArticle

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