Immunobiological analysis of TCR single-chain transgenic mice reveals new possibilities for interaction between CDR3α and an antigenic peptide bound to MHC class I

W. Zhang, S. Honda, F. Wang, Teresa P. DiLorenzo, A. M. Kalergis, D. A. Ostrov, S. G. Nathenson

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Abstract

The interaction between TCRs and peptides presented by MHC molecules determines the specificity of the T cell-mediated immune response. To elucidate the biologically important structural features of this interaction, we generated TCR β-chain transgenic mice using a TCR derived from a T cell clone specific for the immunodominant peptide of vesicular stomatitis virus (RGYVYQGL, VSV8) presented by H-2Kb. We immunized these mice with VSV8 or analogs substituted at TCR contact residues (positions 1, 4, and 6) and analyzed the CDR3α sequences of the elicited T cells. In VSV8-specific CTLs, we observed a highly conserved residue at position 93 of CDR3α and preferred Jα usage, indicating that multiple residues of CDR3α are critical for recognition of the peptide. Certain substitutions at peptide position 4 induced changes at position 93 and in Jα usage, suggesting a potential interaction between CDR3α and position 4. Cross-reactivity data revealed the foremost importance of the Jα region in determining Ag specificity. Surprisingly, substitution at position 6 of VSV8 to a negatively charged residue induced a change at position 93 of CDR3α to a positively charged residue, suggesting that CDR3α may interact with position 6 in certain circumstances. Analogous interactions between the TCR α-chain and residues in the C-terminal half of the peptide have not yet been revealed by the limited number of TCR/peptide-MHC crystal structures reported to date. The transgenic mouse approach allows hundreds of TCR/peptide-MHC interactions to be examined comparatively easily, thus permitting a wide-ranging analysis of the possibilities for Ag recognition in vivo.

Original languageEnglish (US)
Pages (from-to)4396-4404
Number of pages9
JournalJournal of Immunology
Volume167
Issue number8
StatePublished - Oct 15 2001

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Transgenic Mice
Peptides
vesicular stomatitis virus nucleoprotein (52-59)
T-Cell Antigen Receptor Specificity
T-Lymphocytes
Vesicular Stomatitis
Clone Cells
Viruses

ASJC Scopus subject areas

  • Immunology

Cite this

Immunobiological analysis of TCR single-chain transgenic mice reveals new possibilities for interaction between CDR3α and an antigenic peptide bound to MHC class I. / Zhang, W.; Honda, S.; Wang, F.; DiLorenzo, Teresa P.; Kalergis, A. M.; Ostrov, D. A.; Nathenson, S. G.

In: Journal of Immunology, Vol. 167, No. 8, 15.10.2001, p. 4396-4404.

Research output: Contribution to journalArticle

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abstract = "The interaction between TCRs and peptides presented by MHC molecules determines the specificity of the T cell-mediated immune response. To elucidate the biologically important structural features of this interaction, we generated TCR β-chain transgenic mice using a TCR derived from a T cell clone specific for the immunodominant peptide of vesicular stomatitis virus (RGYVYQGL, VSV8) presented by H-2Kb. We immunized these mice with VSV8 or analogs substituted at TCR contact residues (positions 1, 4, and 6) and analyzed the CDR3α sequences of the elicited T cells. In VSV8-specific CTLs, we observed a highly conserved residue at position 93 of CDR3α and preferred Jα usage, indicating that multiple residues of CDR3α are critical for recognition of the peptide. Certain substitutions at peptide position 4 induced changes at position 93 and in Jα usage, suggesting a potential interaction between CDR3α and position 4. Cross-reactivity data revealed the foremost importance of the Jα region in determining Ag specificity. Surprisingly, substitution at position 6 of VSV8 to a negatively charged residue induced a change at position 93 of CDR3α to a positively charged residue, suggesting that CDR3α may interact with position 6 in certain circumstances. Analogous interactions between the TCR α-chain and residues in the C-terminal half of the peptide have not yet been revealed by the limited number of TCR/peptide-MHC crystal structures reported to date. The transgenic mouse approach allows hundreds of TCR/peptide-MHC interactions to be examined comparatively easily, thus permitting a wide-ranging analysis of the possibilities for Ag recognition in vivo.",
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