Immunization-Elicited Broadly Protective Antibody Reveals Ebolavirus Fusion Loop as a Site of Vulnerability

Xuelian Zhao, Katie A. Howell, Shihua He, Jennifer M. Brannan, Anna Z. Wec, Edgar Davidson, Hannah L. Turner, Chi I. Chiang, Lin Lei, J. Maximilian Fels, Hong Vu, Sergey Shulenin, Ashley N. Turonis, Ana I. Kuehne, Guodong Liu, Mi Ta, Yimeng Wang, Christopher Sundling, Yongli Xiao, Jennifer S. Spence & 8 others Benjamin J. Doranz, Frederick W. Holtsberg, Andrew B. Ward, Kartik Chandran, John M. Dye, Xiangguo Qiu, Yuxing Li, M. Javad Aman

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

While neutralizing antibodies are highly effective against ebolavirus infections, current experimental ebolavirus vaccines primarily elicit species-specific antibody responses. Here, we describe an immunization-elicited macaque antibody (CA45) that clamps the internal fusion loop with the N terminus of the ebolavirus glycoproteins (GPs) and potently neutralizes Ebola, Sudan, Bundibugyo, and Reston viruses. CA45, alone or in combination with an antibody that blocks receptor binding, provided full protection against all pathogenic ebolaviruses in mice, guinea pigs, and ferrets. Analysis of memory B cells from the immunized macaque suggests that elicitation of broadly neutralizing antibodies (bNAbs) for ebolaviruses is possible but difficult, potentially due to the rarity of bNAb clones and their precursors. Unexpectedly, germline-reverted CA45, while exhibiting negligible binding to full-length GP, bound a proteolytically remodeled GP with picomolar affinity, suggesting that engineered ebolavirus vaccines could trigger rare bNAb precursors more robustly. These findings have important implications for developing pan-ebolavirus vaccine and immunotherapeutic cocktails.

Original languageEnglish (US)
Pages (from-to)891-904.e15
JournalCell
Volume169
Issue number5
DOIs
StatePublished - May 18 2017

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Ebolavirus
Immunization
Glycoproteins
Fusion reactions
Vaccines
Neutralizing Antibodies
Antibodies
Clamping devices
Viruses
Cells
Macaca
Data storage equipment
Ferrets
Antibody Formation
Guinea Pigs
B-Lymphocytes
Clone Cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Zhao, X., Howell, K. A., He, S., Brannan, J. M., Wec, A. Z., Davidson, E., ... Aman, M. J. (2017). Immunization-Elicited Broadly Protective Antibody Reveals Ebolavirus Fusion Loop as a Site of Vulnerability. Cell, 169(5), 891-904.e15. https://doi.org/10.1016/j.cell.2017.04.038

Immunization-Elicited Broadly Protective Antibody Reveals Ebolavirus Fusion Loop as a Site of Vulnerability. / Zhao, Xuelian; Howell, Katie A.; He, Shihua; Brannan, Jennifer M.; Wec, Anna Z.; Davidson, Edgar; Turner, Hannah L.; Chiang, Chi I.; Lei, Lin; Fels, J. Maximilian; Vu, Hong; Shulenin, Sergey; Turonis, Ashley N.; Kuehne, Ana I.; Liu, Guodong; Ta, Mi; Wang, Yimeng; Sundling, Christopher; Xiao, Yongli; Spence, Jennifer S.; Doranz, Benjamin J.; Holtsberg, Frederick W.; Ward, Andrew B.; Chandran, Kartik; Dye, John M.; Qiu, Xiangguo; Li, Yuxing; Aman, M. Javad.

In: Cell, Vol. 169, No. 5, 18.05.2017, p. 891-904.e15.

Research output: Contribution to journalArticle

Zhao, X, Howell, KA, He, S, Brannan, JM, Wec, AZ, Davidson, E, Turner, HL, Chiang, CI, Lei, L, Fels, JM, Vu, H, Shulenin, S, Turonis, AN, Kuehne, AI, Liu, G, Ta, M, Wang, Y, Sundling, C, Xiao, Y, Spence, JS, Doranz, BJ, Holtsberg, FW, Ward, AB, Chandran, K, Dye, JM, Qiu, X, Li, Y & Aman, MJ 2017, 'Immunization-Elicited Broadly Protective Antibody Reveals Ebolavirus Fusion Loop as a Site of Vulnerability', Cell, vol. 169, no. 5, pp. 891-904.e15. https://doi.org/10.1016/j.cell.2017.04.038
Zhao, Xuelian ; Howell, Katie A. ; He, Shihua ; Brannan, Jennifer M. ; Wec, Anna Z. ; Davidson, Edgar ; Turner, Hannah L. ; Chiang, Chi I. ; Lei, Lin ; Fels, J. Maximilian ; Vu, Hong ; Shulenin, Sergey ; Turonis, Ashley N. ; Kuehne, Ana I. ; Liu, Guodong ; Ta, Mi ; Wang, Yimeng ; Sundling, Christopher ; Xiao, Yongli ; Spence, Jennifer S. ; Doranz, Benjamin J. ; Holtsberg, Frederick W. ; Ward, Andrew B. ; Chandran, Kartik ; Dye, John M. ; Qiu, Xiangguo ; Li, Yuxing ; Aman, M. Javad. / Immunization-Elicited Broadly Protective Antibody Reveals Ebolavirus Fusion Loop as a Site of Vulnerability. In: Cell. 2017 ; Vol. 169, No. 5. pp. 891-904.e15.
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