Immobilization of horseradish peroxidase on O-carboxymethylated chitosan/sol-gel matrix

Qin Xu; Chun Mao; Ni Na Liu; Jun Jie Zhu; Jian Shen

doi:10.1016/j.reactfunctpolym.2005.11.015

Immobilization of horseradish peroxidase on O-carboxymethylated chitosan/sol-gel matrix

Qin Xu, Chun Mao, Ni Na Liu, Jun Jie Zhu, Jian Shen

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

A water-soluble chitosan derivation O-carboxymethyl chitosan (CMCS) was developed for the efficient immobilization of horseradish peroxidase (HRP) to construct a H₂O₂ biosensor. Fourier Transform Infrared (FT-IR) spectrophotometer, Atomic Force Microscope (AFM) and electrochemical method were used to characterize the biosensor. The biosensor was used to determine hydrogen peroxide (H₂O₂) quantitatively in the presence of methylene blue which was used as a mediator to transfer electrons between the electrode and HRP. It offered a linear range of 2.0 × 10^-6-3.7 × 10^-5 and 4.2 × 10^-5-4.57 × 10^-4 M, with a detection limit of 5.32 × 10^-7 M estimated at a signal-to-noise ration of 3. The apparent Michaelis-Menten constant (K_M^app) of the biosensor was 3.544 × 10^-5 M.

Original language	English (US)
Pages (from-to)	863-870
Number of pages	8
Journal	Reactive and Functional Polymers
Volume	66
Issue number	8
DOIs	https://doi.org/10.1016/j.reactfunctpolym.2005.11.015
State	Published - Aug 2006
Externally published	Yes

Keywords

Biosensor
Carboxymethylate chitosan
HRP
Immobilization
Water-soluble

ASJC Scopus subject areas

General Chemistry
Environmental Chemistry
Biochemistry
General Chemical Engineering
Polymers and Plastics
Materials Chemistry

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10.1016/j.reactfunctpolym.2005.11.015

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title = "Immobilization of horseradish peroxidase on O-carboxymethylated chitosan/sol-gel matrix",

abstract = "A water-soluble chitosan derivation O-carboxymethyl chitosan (CMCS) was developed for the efficient immobilization of horseradish peroxidase (HRP) to construct a H2O2 biosensor. Fourier Transform Infrared (FT-IR) spectrophotometer, Atomic Force Microscope (AFM) and electrochemical method were used to characterize the biosensor. The biosensor was used to determine hydrogen peroxide (H2O2) quantitatively in the presence of methylene blue which was used as a mediator to transfer electrons between the electrode and HRP. It offered a linear range of 2.0 × 10-6-3.7 × 10-5 and 4.2 × 10-5-4.57 × 10-4 M, with a detection limit of 5.32 × 10-7 M estimated at a signal-to-noise ration of 3. The apparent Michaelis-Menten constant (KMapp) of the biosensor was 3.544 × 10-5 M.",

keywords = "Biosensor, Carboxymethylate chitosan, HRP, Immobilization, Water-soluble",

author = "Qin Xu and Chun Mao and Liu, {Ni Na} and Zhu, {Jun Jie} and Jian Shen",

note = "Funding Information: This work is supported by the National Natural Science Foundation of China (Grant Nos. 20325516, 20575026), the Foundation of State Key state Laboratory of Electroanalytical Chemistry from Changchun Institute of Applied Chemistry and 973 Project (2004CB520804).",

year = "2006",

month = aug,

doi = "10.1016/j.reactfunctpolym.2005.11.015",

language = "English (US)",

volume = "66",

pages = "863--870",

journal = "Reactive and Functional Polymers",

issn = "1381-5148",

publisher = "Elsevier",

number = "8",

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TY - JOUR

T1 - Immobilization of horseradish peroxidase on O-carboxymethylated chitosan/sol-gel matrix

AU - Xu, Qin

AU - Mao, Chun

AU - Liu, Ni Na

AU - Zhu, Jun Jie

AU - Shen, Jian

N1 - Funding Information: This work is supported by the National Natural Science Foundation of China (Grant Nos. 20325516, 20575026), the Foundation of State Key state Laboratory of Electroanalytical Chemistry from Changchun Institute of Applied Chemistry and 973 Project (2004CB520804).

PY - 2006/8

Y1 - 2006/8

N2 - A water-soluble chitosan derivation O-carboxymethyl chitosan (CMCS) was developed for the efficient immobilization of horseradish peroxidase (HRP) to construct a H2O2 biosensor. Fourier Transform Infrared (FT-IR) spectrophotometer, Atomic Force Microscope (AFM) and electrochemical method were used to characterize the biosensor. The biosensor was used to determine hydrogen peroxide (H2O2) quantitatively in the presence of methylene blue which was used as a mediator to transfer electrons between the electrode and HRP. It offered a linear range of 2.0 × 10-6-3.7 × 10-5 and 4.2 × 10-5-4.57 × 10-4 M, with a detection limit of 5.32 × 10-7 M estimated at a signal-to-noise ration of 3. The apparent Michaelis-Menten constant (KMapp) of the biosensor was 3.544 × 10-5 M.

AB - A water-soluble chitosan derivation O-carboxymethyl chitosan (CMCS) was developed for the efficient immobilization of horseradish peroxidase (HRP) to construct a H2O2 biosensor. Fourier Transform Infrared (FT-IR) spectrophotometer, Atomic Force Microscope (AFM) and electrochemical method were used to characterize the biosensor. The biosensor was used to determine hydrogen peroxide (H2O2) quantitatively in the presence of methylene blue which was used as a mediator to transfer electrons between the electrode and HRP. It offered a linear range of 2.0 × 10-6-3.7 × 10-5 and 4.2 × 10-5-4.57 × 10-4 M, with a detection limit of 5.32 × 10-7 M estimated at a signal-to-noise ration of 3. The apparent Michaelis-Menten constant (KMapp) of the biosensor was 3.544 × 10-5 M.

KW - Biosensor

KW - Carboxymethylate chitosan

KW - HRP

KW - Immobilization

KW - Water-soluble

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JO - Reactive and Functional Polymers

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ER -

Immobilization of horseradish peroxidase on O-carboxymethylated chitosan/sol-gel matrix

Abstract

Keywords

ASJC Scopus subject areas

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