Immobilization of horseradish peroxidase on O-carboxymethylated chitosan/sol-gel matrix

Qin Xu, Chun Mao, Ni Na Liu, Jun Jie Zhu, Jian Shen

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

A water-soluble chitosan derivation O-carboxymethyl chitosan (CMCS) was developed for the efficient immobilization of horseradish peroxidase (HRP) to construct a H2O2 biosensor. Fourier Transform Infrared (FT-IR) spectrophotometer, Atomic Force Microscope (AFM) and electrochemical method were used to characterize the biosensor. The biosensor was used to determine hydrogen peroxide (H2O2) quantitatively in the presence of methylene blue which was used as a mediator to transfer electrons between the electrode and HRP. It offered a linear range of 2.0 × 10-6-3.7 × 10-5 and 4.2 × 10-5-4.57 × 10-4 M, with a detection limit of 5.32 × 10-7 M estimated at a signal-to-noise ration of 3. The apparent Michaelis-Menten constant (KMapp) of the biosensor was 3.544 × 10-5 M.

Original languageEnglish (US)
Pages (from-to)863-870
Number of pages8
JournalReactive and Functional Polymers
Volume66
Issue number8
DOIs
StatePublished - Aug 2006
Externally publishedYes

Fingerprint

electrochemical method
Chitosan
Biosensing Techniques
Polymethyl Methacrylate
Horseradish Peroxidase
Biosensors
Immobilization
immobilization
hydrogen peroxide
Fourier transform
Sol-gels
electrode
gel
Gels
electron
matrix
Infrared spectrophotometers
water
Methylene Blue
Fourier Analysis

Keywords

  • Biosensor
  • Carboxymethylate chitosan
  • HRP
  • Immobilization
  • Water-soluble

ASJC Scopus subject areas

  • Organic Chemistry
  • Polymers and Plastics

Cite this

Immobilization of horseradish peroxidase on O-carboxymethylated chitosan/sol-gel matrix. / Xu, Qin; Mao, Chun; Liu, Ni Na; Zhu, Jun Jie; Shen, Jian.

In: Reactive and Functional Polymers, Vol. 66, No. 8, 08.2006, p. 863-870.

Research output: Contribution to journalArticle

Xu, Qin ; Mao, Chun ; Liu, Ni Na ; Zhu, Jun Jie ; Shen, Jian. / Immobilization of horseradish peroxidase on O-carboxymethylated chitosan/sol-gel matrix. In: Reactive and Functional Polymers. 2006 ; Vol. 66, No. 8. pp. 863-870.
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