Imaging the glycome in living systems

Boyangzi Li, Feiyan Mock, Peng Wu

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Scopus citations

Abstract

The glycome, the full complement of glycans that cells produce, is an attractive target for molecular imaging. Imaging of the glycome in living systems has recently been enabled via bioorthogonal chemical reporter-based approaches. In this chapter, we describe two approaches to introduce bioorthogonal chemical reporters (tags) onto cell surface fucosylated glycans and glycans bearing LacNAc disaccharides, respectively. The tagged glycans can then be conjugated to imaging probes via bioorthogonal click chemistry. Similar approaches can be extended to image other sectors of the glycome in living systems.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages401-419
Number of pages19
DOIs
StatePublished - 2012

Publication series

NameMethods in Enzymology
Volume505
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • bioorthogonal
  • chemoenzymatic labeling
  • glycans
  • metabolic labeling

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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