Bromopyruvate inactivates 2-keto-3-deoxygluconate-6-P aldolase by a mechanism in which the reagent is incorporated by esterification. A tryptic peptide derived from inactivated enzyme has the sequence Thr-Leu-Glu*-Val-Thr-Leu-Arg. Derivatization of the γ-carboxyl of the single glutamate by bromopyruvate was confirmed by Lossen rearrangement in which the glutamate γ-ester was converted to 2,4-diamino butyrate.
|Original language||English (US)|
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Oct 16 1978|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology