Identification of the bromopyruvate-sensitive glutamate within the active site of 2-keto-3-deoxygluconate-6-P aldolase

H. Paul Meloche, Claire T. Monti, Ruth A. Hogue-Angeletti

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Bromopyruvate inactivates 2-keto-3-deoxygluconate-6-P aldolase by a mechanism in which the reagent is incorporated by esterification. A tryptic peptide derived from inactivated enzyme has the sequence Thr-Leu-Glu*-Val-Thr-Leu-Arg. Derivatization of the γ-carboxyl of the single glutamate by bromopyruvate was confirmed by Lossen rearrangement in which the glutamate γ-ester was converted to 2,4-diamino butyrate.

Original languageEnglish (US)
Pages (from-to)589-594
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume84
Issue number3
DOIs
StatePublished - Oct 16 1978

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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