Identification of Ski as a target for Aurora A kinase

Jocelyn Mosquera, Ricardo Armisen, Hongling Zhao, Diego A. Rojas, Edio Maldonado, Julio C. Tapia, Alicia Colombo, Michael J. Hayman, Katherine Marcelain

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Ski is a negative regulator of the transforming growth factor-β and other signalling pathways. The absence of SKI in mouse fibroblasts leads to chromosome segregation defects and genomic instability, suggesting a role for Ski during mitosis. At this stage, Ski is phosphorylated but to date little is known about the kinases involved in this process. Here, we show that Aurora A kinase is able to phosphorylate Ski in vitro. In vivo, Aurora A and Ski co-localized at the centrosomes and co-immunoprecipitated. Conversely, a C-terminal truncation mutant of Ski (SkiΔ491-728) lacking a coiled-coil domain, displayed decreased centrosomal localization. This mutant no longer co-immunoprecipitated with Aurora-A in vivo, but was still phosphorylated in vitro, indicating that the Ski-Aurora A interaction takes place at the centrosomes. These data identify Ski as a novel target of Aurora A and contribute to an understanding of the role of these proteins in the mitotic process.

Original languageEnglish (US)
Pages (from-to)539-543
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume409
Issue number3
DOIs
StatePublished - Jun 10 2011
Externally publishedYes

Fingerprint

Aurora Kinase A
Centrosome
Transforming Growth Factors
Fibroblasts
Chromosomes
Phosphotransferases
Chromosome Segregation
Defects
Genomic Instability
Mitosis
Proteins
In Vitro Techniques

Keywords

  • Aurora A
  • Ski
  • Ski phosphorylation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Mosquera, J., Armisen, R., Zhao, H., Rojas, D. A., Maldonado, E., Tapia, J. C., ... Marcelain, K. (2011). Identification of Ski as a target for Aurora A kinase. Biochemical and Biophysical Research Communications, 409(3), 539-543. https://doi.org/10.1016/j.bbrc.2011.05.040

Identification of Ski as a target for Aurora A kinase. / Mosquera, Jocelyn; Armisen, Ricardo; Zhao, Hongling; Rojas, Diego A.; Maldonado, Edio; Tapia, Julio C.; Colombo, Alicia; Hayman, Michael J.; Marcelain, Katherine.

In: Biochemical and Biophysical Research Communications, Vol. 409, No. 3, 10.06.2011, p. 539-543.

Research output: Contribution to journalArticle

Mosquera, J, Armisen, R, Zhao, H, Rojas, DA, Maldonado, E, Tapia, JC, Colombo, A, Hayman, MJ & Marcelain, K 2011, 'Identification of Ski as a target for Aurora A kinase', Biochemical and Biophysical Research Communications, vol. 409, no. 3, pp. 539-543. https://doi.org/10.1016/j.bbrc.2011.05.040
Mosquera, Jocelyn ; Armisen, Ricardo ; Zhao, Hongling ; Rojas, Diego A. ; Maldonado, Edio ; Tapia, Julio C. ; Colombo, Alicia ; Hayman, Michael J. ; Marcelain, Katherine. / Identification of Ski as a target for Aurora A kinase. In: Biochemical and Biophysical Research Communications. 2011 ; Vol. 409, No. 3. pp. 539-543.
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