Identification of Ski as a target for Aurora A kinase

Jocelyn Mosquera; Ricardo Armisen; Hongling Zhao; Diego A. Rojas; Edio Maldonado; Julio C. Tapia; Alicia Colombo; Michael J. Hayman; Katherine Marcelain

doi:10.1016/j.bbrc.2011.05.040

Identification of Ski as a target for Aurora A kinase

Jocelyn Mosquera, Ricardo Armisen, Hongling Zhao, Diego A. Rojas, Edio Maldonado, Julio C. Tapia, Alicia Colombo, Michael J. Hayman, Katherine Marcelain

Developmental & Molecular Biology

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Ski is a negative regulator of the transforming growth factor-β and other signalling pathways. The absence of SKI in mouse fibroblasts leads to chromosome segregation defects and genomic instability, suggesting a role for Ski during mitosis. At this stage, Ski is phosphorylated but to date little is known about the kinases involved in this process. Here, we show that Aurora A kinase is able to phosphorylate Ski in vitro. In vivo, Aurora A and Ski co-localized at the centrosomes and co-immunoprecipitated. Conversely, a C-terminal truncation mutant of Ski (SkiΔ491-728) lacking a coiled-coil domain, displayed decreased centrosomal localization. This mutant no longer co-immunoprecipitated with Aurora-A in vivo, but was still phosphorylated in vitro, indicating that the Ski-Aurora A interaction takes place at the centrosomes. These data identify Ski as a novel target of Aurora A and contribute to an understanding of the role of these proteins in the mitotic process.

Original language	English (US)
Pages (from-to)	539-543
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	409
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2011.05.040
State	Published - Jun 10 2011

Keywords

Aurora A
Ski
Ski phosphorylation

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Identification of Ski as a target for Aurora A kinase. / Mosquera, Jocelyn; Armisen, Ricardo; Zhao, Hongling; Rojas, Diego A.; Maldonado, Edio; Tapia, Julio C.; Colombo, Alicia; Hayman, Michael J.; Marcelain, Katherine.

In: Biochemical and Biophysical Research Communications, Vol. 409, No. 3, 10.06.2011, p. 539-543.

Research output: Contribution to journal › Article › peer-review

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title = "Identification of Ski as a target for Aurora A kinase",

abstract = "Ski is a negative regulator of the transforming growth factor-β and other signalling pathways. The absence of SKI in mouse fibroblasts leads to chromosome segregation defects and genomic instability, suggesting a role for Ski during mitosis. At this stage, Ski is phosphorylated but to date little is known about the kinases involved in this process. Here, we show that Aurora A kinase is able to phosphorylate Ski in vitro. In vivo, Aurora A and Ski co-localized at the centrosomes and co-immunoprecipitated. Conversely, a C-terminal truncation mutant of Ski (SkiΔ491-728) lacking a coiled-coil domain, displayed decreased centrosomal localization. This mutant no longer co-immunoprecipitated with Aurora-A in vivo, but was still phosphorylated in vitro, indicating that the Ski-Aurora A interaction takes place at the centrosomes. These data identify Ski as a novel target of Aurora A and contribute to an understanding of the role of these proteins in the mitotic process.",

keywords = "Aurora A, Ski, Ski phosphorylation",

author = "Jocelyn Mosquera and Ricardo Armisen and Hongling Zhao and Rojas, {Diego A.} and Edio Maldonado and Tapia, {Julio C.} and Alicia Colombo and Hayman, {Michael J.} and Katherine Marcelain",

note = "Funding Information: We thank Dr. Jin Q. Cheng from H. Lee Moffitt Cancer Center for kindly providing the HA-Aurora A construct and Dr. Mar{\'i}a Julieta Gonz{\'a}lez, from Instituto de Ciencias Biom{\'e}dicas, Universidad de Chile, for generously providing key reagents for this paper. This work was supported by US Public Health Service Grant CA42573 from the National Cancer Institute (M.J.H.), Vicerrector{\'i}a de Investigaci{\'o}n y Desarrollo (VID) Grant I07/11-2 from Universidad de Chile (K.M.) and Grants 15010006 from FONDAP (Fondo de Financiamiento de Centros de Excelencia en Investigaci{\'o}n) and 11070116 from FONDECYT (J.C.T.), Chile. ",

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AU - Mosquera, Jocelyn

AU - Armisen, Ricardo

AU - Zhao, Hongling

AU - Rojas, Diego A.

AU - Maldonado, Edio

AU - Tapia, Julio C.

AU - Colombo, Alicia

AU - Hayman, Michael J.

AU - Marcelain, Katherine

N1 - Funding Information: We thank Dr. Jin Q. Cheng from H. Lee Moffitt Cancer Center for kindly providing the HA-Aurora A construct and Dr. María Julieta González, from Instituto de Ciencias Biomédicas, Universidad de Chile, for generously providing key reagents for this paper. This work was supported by US Public Health Service Grant CA42573 from the National Cancer Institute (M.J.H.), Vicerrectoría de Investigación y Desarrollo (VID) Grant I07/11-2 from Universidad de Chile (K.M.) and Grants 15010006 from FONDAP (Fondo de Financiamiento de Centros de Excelencia en Investigación) and 11070116 from FONDECYT (J.C.T.), Chile.

PY - 2011/6/10

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N2 - Ski is a negative regulator of the transforming growth factor-β and other signalling pathways. The absence of SKI in mouse fibroblasts leads to chromosome segregation defects and genomic instability, suggesting a role for Ski during mitosis. At this stage, Ski is phosphorylated but to date little is known about the kinases involved in this process. Here, we show that Aurora A kinase is able to phosphorylate Ski in vitro. In vivo, Aurora A and Ski co-localized at the centrosomes and co-immunoprecipitated. Conversely, a C-terminal truncation mutant of Ski (SkiΔ491-728) lacking a coiled-coil domain, displayed decreased centrosomal localization. This mutant no longer co-immunoprecipitated with Aurora-A in vivo, but was still phosphorylated in vitro, indicating that the Ski-Aurora A interaction takes place at the centrosomes. These data identify Ski as a novel target of Aurora A and contribute to an understanding of the role of these proteins in the mitotic process.

AB - Ski is a negative regulator of the transforming growth factor-β and other signalling pathways. The absence of SKI in mouse fibroblasts leads to chromosome segregation defects and genomic instability, suggesting a role for Ski during mitosis. At this stage, Ski is phosphorylated but to date little is known about the kinases involved in this process. Here, we show that Aurora A kinase is able to phosphorylate Ski in vitro. In vivo, Aurora A and Ski co-localized at the centrosomes and co-immunoprecipitated. Conversely, a C-terminal truncation mutant of Ski (SkiΔ491-728) lacking a coiled-coil domain, displayed decreased centrosomal localization. This mutant no longer co-immunoprecipitated with Aurora-A in vivo, but was still phosphorylated in vitro, indicating that the Ski-Aurora A interaction takes place at the centrosomes. These data identify Ski as a novel target of Aurora A and contribute to an understanding of the role of these proteins in the mitotic process.

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