Identification of Ski as a target for Aurora A kinase

Jocelyn Mosquera, Ricardo Armisen, Hongling Zhao, Diego A. Rojas, Edio Maldonado, Julio C. Tapia, Alicia Colombo, Michael J. Hayman, Katherine Marcelain

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Ski is a negative regulator of the transforming growth factor-β and other signalling pathways. The absence of SKI in mouse fibroblasts leads to chromosome segregation defects and genomic instability, suggesting a role for Ski during mitosis. At this stage, Ski is phosphorylated but to date little is known about the kinases involved in this process. Here, we show that Aurora A kinase is able to phosphorylate Ski in vitro. In vivo, Aurora A and Ski co-localized at the centrosomes and co-immunoprecipitated. Conversely, a C-terminal truncation mutant of Ski (SkiΔ491-728) lacking a coiled-coil domain, displayed decreased centrosomal localization. This mutant no longer co-immunoprecipitated with Aurora-A in vivo, but was still phosphorylated in vitro, indicating that the Ski-Aurora A interaction takes place at the centrosomes. These data identify Ski as a novel target of Aurora A and contribute to an understanding of the role of these proteins in the mitotic process.

Original languageEnglish (US)
Pages (from-to)539-543
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume409
Issue number3
DOIs
StatePublished - Jun 10 2011
Externally publishedYes

Keywords

  • Aurora A
  • Ski
  • Ski phosphorylation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Mosquera, J., Armisen, R., Zhao, H., Rojas, D. A., Maldonado, E., Tapia, J. C., Colombo, A., Hayman, M. J., & Marcelain, K. (2011). Identification of Ski as a target for Aurora A kinase. Biochemical and Biophysical Research Communications, 409(3), 539-543. https://doi.org/10.1016/j.bbrc.2011.05.040