Identification of Ski as a target for Aurora A kinase

Jocelyn Mosquera; Ricardo Armisen; Hongling Zhao; Diego A. Rojas; Edio Maldonado; Julio C. Tapia; Alicia Colombo; Michael J. Hayman; Katherine Marcelain

doi:10.1016/j.bbrc.2011.05.040

Identification of Ski as a target for Aurora A kinase

Jocelyn Mosquera, Ricardo Armisen, Hongling Zhao, Diego A. Rojas, Edio Maldonado, Julio C. Tapia, Alicia Colombo, Michael J. Hayman, Katherine Marcelain

Research output: Contribution to journal › Article

9 Scopus citations

Abstract

Ski is a negative regulator of the transforming growth factor-β and other signalling pathways. The absence of SKI in mouse fibroblasts leads to chromosome segregation defects and genomic instability, suggesting a role for Ski during mitosis. At this stage, Ski is phosphorylated but to date little is known about the kinases involved in this process. Here, we show that Aurora A kinase is able to phosphorylate Ski in vitro. In vivo, Aurora A and Ski co-localized at the centrosomes and co-immunoprecipitated. Conversely, a C-terminal truncation mutant of Ski (SkiΔ491-728) lacking a coiled-coil domain, displayed decreased centrosomal localization. This mutant no longer co-immunoprecipitated with Aurora-A in vivo, but was still phosphorylated in vitro, indicating that the Ski-Aurora A interaction takes place at the centrosomes. These data identify Ski as a novel target of Aurora A and contribute to an understanding of the role of these proteins in the mitotic process.

Original language	English (US)
Pages (from-to)	539-543
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	409
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2011.05.040
State	Published - Jun 10 2011
Externally published	Yes

Keywords

Aurora A
Ski
Ski phosphorylation

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Mosquera, J., Armisen, R., Zhao, H., Rojas, D. A., Maldonado, E., Tapia, J. C., Colombo, A., Hayman, M. J., & Marcelain, K. (2011). Identification of Ski as a target for Aurora A kinase. Biochemical and Biophysical Research Communications, 409(3), 539-543. https://doi.org/10.1016/j.bbrc.2011.05.040

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title = "Identification of Ski as a target for Aurora A kinase",

abstract = "Ski is a negative regulator of the transforming growth factor-β and other signalling pathways. The absence of SKI in mouse fibroblasts leads to chromosome segregation defects and genomic instability, suggesting a role for Ski during mitosis. At this stage, Ski is phosphorylated but to date little is known about the kinases involved in this process. Here, we show that Aurora A kinase is able to phosphorylate Ski in vitro. In vivo, Aurora A and Ski co-localized at the centrosomes and co-immunoprecipitated. Conversely, a C-terminal truncation mutant of Ski (SkiΔ491-728) lacking a coiled-coil domain, displayed decreased centrosomal localization. This mutant no longer co-immunoprecipitated with Aurora-A in vivo, but was still phosphorylated in vitro, indicating that the Ski-Aurora A interaction takes place at the centrosomes. These data identify Ski as a novel target of Aurora A and contribute to an understanding of the role of these proteins in the mitotic process.",

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author = "Jocelyn Mosquera and Ricardo Armisen and Hongling Zhao and Rojas, {Diego A.} and Edio Maldonado and Tapia, {Julio C.} and Alicia Colombo and Hayman, {Michael J.} and Katherine Marcelain",

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AU - Mosquera, Jocelyn

AU - Armisen, Ricardo

AU - Zhao, Hongling

AU - Rojas, Diego A.

AU - Maldonado, Edio

AU - Tapia, Julio C.

AU - Colombo, Alicia

AU - Hayman, Michael J.

AU - Marcelain, Katherine

PY - 2011/6/10

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N2 - Ski is a negative regulator of the transforming growth factor-β and other signalling pathways. The absence of SKI in mouse fibroblasts leads to chromosome segregation defects and genomic instability, suggesting a role for Ski during mitosis. At this stage, Ski is phosphorylated but to date little is known about the kinases involved in this process. Here, we show that Aurora A kinase is able to phosphorylate Ski in vitro. In vivo, Aurora A and Ski co-localized at the centrosomes and co-immunoprecipitated. Conversely, a C-terminal truncation mutant of Ski (SkiΔ491-728) lacking a coiled-coil domain, displayed decreased centrosomal localization. This mutant no longer co-immunoprecipitated with Aurora-A in vivo, but was still phosphorylated in vitro, indicating that the Ski-Aurora A interaction takes place at the centrosomes. These data identify Ski as a novel target of Aurora A and contribute to an understanding of the role of these proteins in the mitotic process.

AB - Ski is a negative regulator of the transforming growth factor-β and other signalling pathways. The absence of SKI in mouse fibroblasts leads to chromosome segregation defects and genomic instability, suggesting a role for Ski during mitosis. At this stage, Ski is phosphorylated but to date little is known about the kinases involved in this process. Here, we show that Aurora A kinase is able to phosphorylate Ski in vitro. In vivo, Aurora A and Ski co-localized at the centrosomes and co-immunoprecipitated. Conversely, a C-terminal truncation mutant of Ski (SkiΔ491-728) lacking a coiled-coil domain, displayed decreased centrosomal localization. This mutant no longer co-immunoprecipitated with Aurora-A in vivo, but was still phosphorylated in vitro, indicating that the Ski-Aurora A interaction takes place at the centrosomes. These data identify Ski as a novel target of Aurora A and contribute to an understanding of the role of these proteins in the mitotic process.

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KW - Ski

KW - Ski phosphorylation

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